Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,489,320	A→G	G287G (GGT→GGC)	argD ←	N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,489,320	0	A	G	100.0%	99.1 / NA	29	G287G (GGT→GGC)	argD	N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/13);  total (16/13)

TAAATACATCGTACTGCTGATCGATCTTCTGCAGATGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCG  >  NC_000913/3489184‑3489449                                                                                                                                         |                                                                                                                                  tAAATACATCGTACTGCTGATCGATCTTCTGCAGATGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTGccg                                                                                                                                <  2:664574/140‑1 (MQ=255) tAAATACATCGTACTGCTGATCGATCTTCTGCAGATGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTGccg                                                                                                                                <  1:440356/140‑1 (MQ=255)    aTACATCGTACTGCTGATCGATCTTCTGCAGATGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTGccgccg                                                                                                                             >  2:597935/1‑140 (MQ=255)        aTCGTACTGCTGATCGATCTTCTGCAGATGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCACCCGCTACTGCGCAGGCCAGAGGATTGCCGCCGTAgg                                                                                                                         >  1:212092/1‑140 (MQ=255)                 ctgATCGATCTTCTGCAGATGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGt                                                                                                                >  1:148398/1‑140 (MQ=255)                           ttctGCAGATGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGACAGCGGATTGCCGCCGAAGGTGGAACCGTGAGAACCAgg                                                                                                      >  2:253014/1‑140 (MQ=255)                                   aTGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACg                                                                                              >  1:666515/1‑140 (MQ=255)                                   aTGGTCAACAAAACGCGGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAGCg                                                                                              <  2:16807/140‑1 (MQ=255)                                                    ggCATTTCGCCTGAATGACTTCAAGCACTTCCGGGGTATCGATGATATAAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTccgcc                                                                             <  2:262755/140‑1 (MQ=255)                                                        tttCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTgg                                                                         >  1:600514/1‑140 (MQ=255)                                                                   tGCCTTCCAGCACTTCAGGGGTTTTTATGTTATCAAAGGCCGCCCCCGCTACTGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGgcg                                                              <  1:253014/140‑1 (MQ=255)                                                                              aCTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGaa                                                   >  2:629508/1‑140 (MQ=255)                                                                                  cAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAgccg                                               <  2:600514/140‑1 (MQ=255)                                                                                          ttGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTaa                                       >  1:47837/1‑140 (MQ=255)                                                                                          ttGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTaa                                       >  2:575996/1‑140 (MQ=255)                                                                                          ttGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTaa                                       >  2:610424/1‑140 (MQ=255)                                                                                                atatCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCttt                                 >  1:149647/1‑140 (MQ=255)                                                                                                      aaCGCCGCCCCCGCTACTGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCaga                           <  2:4398/140‑1 (MQ=255)                                                                                                        cgccgcCCCCGCTACTGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAgg                         <  2:463656/140‑1 (MQ=255)                                                                                                           cgccCCCGCTACTGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGCAAGCCGCCTCCTACCGCTTTCGCAGAGGTCa                      >  2:267114/1‑140 (MQ=255)                                                                                                            gccCCCGCTACTGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAg                     >  1:388500/1‑140 (MQ=255)                                                                                                                       tGCACAGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAAACGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCg          <  2:148398/140‑1 (MQ=255)                                                                                                                          acaGGCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTaa       <  2:388500/140‑1 (MQ=255)                                                                                                                            aGGCCAGAGGATTGCCGCCGTAGGTGGAACCCTGATAACTAGGATGAACCGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCACAGAGGTCAGAATATCCGGCGTAACg     <  2:208364/140‑1 (MQ=255)                                                                                                                              gCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGcc   >  1:652969/1‑140 (MQ=255)                                                                                                                              gCCAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGcc   >  1:59639/1‑140 (MQ=255)                                                                                                                               ccAGAGGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCg  <  1:597935/140‑1 (MQ=255)                                                                                                                                   agGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACg     <  2:138884/133‑1 (MQ=255)                                                                                                                                   agGATTGCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACg     >  1:138884/1‑133 (MQ=255)                                                                                                                                         |                                                                                                                                  TAAATACATCGTACTGCTGATCGATCTTCTGCAGATGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCG  >  NC_000913/3489184‑3489449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑