Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	375,420	C→T	intergenic (+539/‑39)	mhpE → / → mhpT	4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	375,420	0	C	T	100.0%	135.9 / NA	40	intergenic (+539/‑39)	mhpE/mhpT	4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/18);  total (22/18)

CCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGG  >  NC_000913/375289‑375555                                                                                                                                      |                                                                                                                                        ccTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGa                                                                                                                                 >  1:114630/1‑140 (MQ=255)   tGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGAtt                                                                                                                               >  2:563978/1‑140 (MQ=255)     aTAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCa                                                                                                                             >  1:359080/1‑140 (MQ=255)         gACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTg                                                                                                                         >  1:565602/1‑140 (MQ=255)                  gCGTCGCATCAGGCATCTGCACCCGTCTGCCGGATGCT‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCaa                                                                                                                <  1:221023/140‑1 (MQ=255)                  gCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCaa                                                                                                                <  1:563978/140‑1 (MQ=255)                   cGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAAc                                                                                                               <  2:624785/140‑1 (MQ=255)                     tCGCATCAGGCATCTTCACACGCCTCCCGAATGCG‑‑ATCAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGa                                                                                                             <  1:187262/140‑1 (MQ=255)                       gCATCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATGACGCGGCTGGCAATGTGTTAGTCGCGATTCTCCTGCACTCGCAACGAg                                                                                                            >  2:186646/1‑140 (MQ=17)                        cATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGt                                                                                                          >  2:389514/1‑140 (MQ=255)                         aTCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGtt                                                                                                         <  1:49927/140‑1 (MQ=255)                         aTCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGtt                                                                                                         <  2:114630/140‑1 (MQ=255)                                   tGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCCCGATTCAGCTGCACACGCAACGAGGTTCATCTTATGt                                                                                               >  1:307366/1‑140 (MQ=255)                                    gCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTc                                                                                              <  2:565136/140‑1 (MQ=255)                                     cacaCGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCg                                                                                             >  1:540297/1‑140 (MQ=255)                                        acGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACt                                                                                          <  2:438346/140‑1 (MQ=255)                                               cgcgcgtcG‑‑ATAAGCGTCTTGTACGGCCTACGTTTCGCCCGCAGCCGAAAACTACATCGTTCTGATTTAGCCGCTAGCAATGTATTAGTCGCGATTCCGCTGAGCTCGCAACGGTGTTCATCTTATGTCGACTCGTAccc                                                                                   <  2:556331/133‑1 (MQ=255)                                                     gCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTtcat                                                                             <  2:106001/140‑1 (MQ=255)                                                     gCG‑‑ATAAACGTCTTGTCCGGCCGACATTTCGACCGTAGGCAGTCATTATATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTtcat                                                                             <  2:259276/140‑1 (MQ=255)                                                                   ttGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATccc                                                                 >  1:605074/1‑140 (MQ=255)                                                                                   tCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCAt                                                 <  2:540297/140‑1 (MQ=255)                                                                                    cGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATATCGACTCGTACCCCTTCTTCATCTTCATCCCGCCTGATGCTGACCATc                                                >  2:313131/1‑140 (MQ=255)                                                                                                gTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGtttt                                    >  2:98129/1‑140 (MQ=255)                                                                                                       aaTAGTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCg                             <  1:98129/140‑1 (MQ=255)                                                                                                           gTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTgg                                <  1:568041/133‑1 (MQ=255)                                                                                                           gTTCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTgg                                >  2:568041/1‑133 (MQ=255)                                                                                                            ttCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTg                        >  2:600917/1‑140 (MQ=255)                                                                                                            ttCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCGGATGCTGACCAGCGGGCTTTGTTTTTTGGTCGCTCTg                        >  1:233325/1‑140 (MQ=255)                                                                                                             tCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGa                       >  1:135741/1‑140 (MQ=255)                                                                                                             tCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGa                       >  1:156413/1‑140 (MQ=255)                                                                                                             tCTGATTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTGCATCCCGCCTGACGCTCACCATCGGCCTTTCTTTTTTGGTCGCTCTGa                       >  2:204514/1‑140 (MQ=255)                                                                                                              cTGATTACGCGGCTGCCAATGTCTTGGTCTCGATTCAACTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGAt                      <  1:186646/140‑1 (MQ=255)                                                                                                                 aTTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGTTGACCATCGGGATTGGTTTTTTGGTCGCTCTGATGGa                   >  1:275944/1‑140 (MQ=255)                                                                                                                 aTTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGa                   <  1:389514/140‑1 (MQ=255)                                                                                                                 aTTACGCGGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGa                   >  2:137792/1‑140 (MQ=255)                                                                                                                      gcgGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGc              >  1:504270/1‑140 (MQ=255)                                                                                                                      gcgGCTGGCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCAGCCGACTTTGTTTATTGGTTGCTCTGATGGAAGGGc              >  2:295591/1‑140 (MQ=255)                                                                                                                          cTGGCAATGTATTAGTCGCGATTCACCTGGACTCGCATCGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGa          <  1:249001/140‑1 (MQ=255)                                                                                                                             gCAATGTATTAGTCGCGATTCAGCTGCACTCGCAACGGGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCt       <  1:295591/140‑1 (MQ=255)                                                                                                                             gCAATGTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCACCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCt       >  2:46998/1‑140 (MQ=255)                                                                                                                                  gTATTAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAgg  <  2:135741/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        CCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCG‑‑ATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTCGCAACGAGGTTCATCTTATGTCGACTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGG  >  NC_000913/375289‑375555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑