Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,511,233	G→A	P64S (CCG→TCG)	yhfY ←	conserved protein YhfY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,511,233	0	G	A	100.0%	125.5 / NA	39	P64S (CCG→TCG)	yhfY	conserved protein YhfY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/19);  total (20/19)

GGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTCCTTCAACAACACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGGCTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCA  >  NC_000913/3511102‑3511368                                                                                                                                    |                                                                                                                                        ggCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTCCTTCAACAACACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCTAGTTCTGCCAGTAAGTCGTTAGCCAGCGACTCTAttt                                                                                                                                 >  1:308399/1‑140 (MQ=255) ggCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTCCTTCAACAACACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTAttt                                                                                                                                 >  2:583088/1‑140 (MQ=255) ggCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTCCTTCAACAACACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTAttt                                                                                                                                 >  2:535251/1‑140 (MQ=255) ggCCAACAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTCCTTCAACAACACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCCCCAATGCGGCCAGCAATTCGTTATCCAGCGACTCTAttt                                                                                                                                 >  2:287513/1‑140 (MQ=255)     aGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTCCTTCAACAACACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATttcttc                                                                                                                             <  1:53827/140‑1 (MQ=255)                  cttcACACGGGTTAACTTCCAGTGCGAATTCCTTCAACAACACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTg                                                                                                                <  2:79723/140‑1 (MQ=255)                            gTTAACTTCCAGTGCGAATTCCTTCAACAACACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTg                                                                                                      <  2:451162/140‑1 (MQ=255)                              tAACTTCCAGTGCGACTTCCTTCAATAACGCCTGATGTAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAATTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCa                                                                                                    <  1:317315/140‑1 (MQ=255)                              tAACTTCCAGTGCGAATTCCTTCAACAACACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCa                                                                                                    <  2:161295/140‑1 (MQ=255)                                  ttCCAGTGCGAATTCCTTCAACAACACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCg                                                                                                >  2:583622/1‑140 (MQ=255)                                    ccAGTGCGAATTCCTTCAACAACACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGcc                                                                                              >  1:427610/1‑140 (MQ=255)                                      aGTGCGAATTCCTTCAACAACACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCa                                                                                                    <  1:444526/132‑1 (MQ=255)                                      aGTGCGAATTCCTTCAACAACACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCa                                                                                                    >  2:444526/1‑132 (MQ=255)                                       gTGCGAATTCCTTCAACAACACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCata                                                                                           >  1:566403/1‑140 (MQ=255)                                                 cTTCAACAACACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCg                                                                                 <  1:512205/140‑1 (MQ=255)                                                    caacaaCACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCa                                                                              >  2:472856/1‑140 (MQ=255)                                                      acatctccTGATGCAATGGCACAGCGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACGCGTTATCCAGCGACTCTATTTCTTCACCGCGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATc                                                                            <  2:609292/134‑1 (MQ=255)                                                        aaccCCTGATGCAATTGCACAACGGCTTGCCAGTGTCTGTATTGCGCCCGTTCGGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGt                                                                          <  2:176009/140‑1 (MQ=255)                                                        aacaCCTGATGCAATTGCACAACGGCTTGTCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGt                                                                          <  1:287513/140‑1 (MQ=255)                                                               gATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGGAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGc                                                                   >  2:397992/1‑140 (MQ=255)                                                               gATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGTGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGc                                                                   >  1:473970/1‑140 (MQ=255)                                                                aTGCAATTGCACAACCGCTTGCCAGCGGCTGGATTGCCCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCt                                                                  <  1:180815/140‑1 (MQ=255)                                                                     aTTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGGATCAGTGCGCTCACCAGATGTGTCAGCGCCATCGTTCCTTGCTCACTg                                                             >  2:197315/1‑140 (MQ=255)                                                                            aaCGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCg                                                      <  2:149920/140‑1 (MQ=255)                                                                              cGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGc                                                    >  1:438589/1‑140 (MQ=255)                                                                              cGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGc                                                    >  2:12701/1‑140 (MQ=255)                                                                                gCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAg                                                  >  2:502774/1‑140 (MQ=255)                                                                                 cTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGa                                                 >  2:441141/1‑140 (MQ=255)                                                                                   tGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCAATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATg                                               >  2:377155/1‑140 (MQ=255)                                                                                      cAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCTCCATCGTTCCTTGCGCACTGCGCACCGGCAGATGGCa                                            >  1:290710/1‑140 (MQ=255)                                                                                             tGGATTGCGCCAGTTCTGCCAGTAATTCGATATCCAGTGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGtt                                     <  1:187422/140‑1 (MQ=255)                                                                                                   gcgcCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGt                               <  1:502774/140‑1 (MQ=255)                                                                                                    cgcCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTa                              <  2:30855/140‑1 (MQ=255)                                                                                                    cgcCAGTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTCATCACTGCGCACAGGTAGATGGTACTCTGTTTCCAGTa                              >  1:308407/1‑140 (MQ=255)                                                                                                         gTTCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTg                         <  1:47142/140‑1 (MQ=255)                                                                                                          ttCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGTCAGGTGGCACTCTGTTTCCAGTACGTTga                        >  1:289830/1‑140 (MQ=255)                                                                                                           tCTGCCAGCAACTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTgac                       <  1:583088/140‑1 (MQ=255)                                                                                                                      cTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGcatca            <  2:438589/140‑1 (MQ=255)                                                                                                                      cTCGTTATCCAGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCAATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGcatca            <  1:377155/140‑1 (MQ=255)                                                                                                                         gTTATCCAGCGACTCTTTTTCTTCACCACGTGGTCTGCGGATCAGTGCACTCGCCTTATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGc         <  2:308407/140‑1 (MQ=255)                                                                                                                            aTCCAGCGACTCTATTTCTTCACCACGGTGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCtt      <  2:473970/140‑1 (MQ=255)                                                                                                                                aGCGACTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAAATGGCACTATGTATCCAGTACGTTGGCGACGTGCATCATGCCTTTGCa  >  2:168968/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        GGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTCCTTCAACAACACCTGATGCAATTGCACAACGGCTTGCCAGTGGCTGGATTGCGCCAGTTCTGCCAGCAACTCGTTATCCAGCGGCTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCA  >  NC_000913/3511102‑3511368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑