| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,544,403 | T→C | T148A (ACC→GCC) | bioH ← | pimeloyl‑acyl carrier protein methyl ester esterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,544,403 | 0 | T | C | 95.5% | 64.4 / ‑4.4 | 22 | T148A (ACC→GCC) | bioH | pimeloyl‑acyl carrier protein methyl ester esterase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (11/10); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAG > NC_000913/3544277‑3544541 | gACGGAGATGGGCCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAgg < 1:44782/140‑1 (MQ=255) agaTCGCCCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCg < 1:642325/140‑1 (MQ=255) agaCCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCg < 2:655196/140‑1 (MQ=255) gACCTTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCGCTCCa < 1:25841/140‑1 (MQ=255) ttttCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGACCCCATGGCTTGTAACGCCAGTAACCGCTCCACTGtc > 1:186064/1‑139 (MQ=255) tCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATTCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAatc > 2:471871/1‑140 (MQ=255) ccGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCGCGCCGCTGTACGCTGAAAATCATCACt > 1:234879/1‑140 (MQ=255) gCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGa > 1:488453/1‑140 (MQ=255) cACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTgctgct > 1:17849/1‑140 (MQ=255) gTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGaa > 1:540545/1‑140 (MQ=255) tCAACCTCCGGCATCTGTAACGCCACAACTGTTTTCTCCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGaaa < 1:188104/140‑1 (MQ=255) ccTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATTATTACTGAGTTGCTGCTGAAATccc > 1:211518/1‑140 (MQ=255) ggCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAg > 1:18560/1‑140 (MQ=255) ggCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAg > 1:399455/1‑140 (MQ=255) gCATCGGTAACGCCAGAGCGGTCTTCTTCACTGCCCGCGTGTCCTGGCACGCCGTTTCAGCCCCCATGGCTTGAAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTCCTGAAATCCCGCCAGc < 2:186064/140‑1 (MQ=255) cGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGt > 1:429569/1‑140 (MQ=255) cGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCGGCCAGCAAGTCCGGTTTTATCCCCGGCCACTCGTCAc > 1:288193/1‑140 (MQ=255) gCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACg < 2:18560/140‑1 (MQ=255) gCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACg < 2:36871/140‑1 (MQ=255) gcgcATCCTGGCGCGCCGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCa < 2:488453/140‑1 (MQ=255) tCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCGCCGGCCACTCGTCACGAGCACTaaa > 1:415723/1‑140 (MQ=255) gcCGTTTCAGTCCCCATGGCTTTTAACGCCAGGAATCGCTCCACTGTATGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTga < 2:234879/140‑1 (MQ=255) cGTTTCAGTCCCCATGGCTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTgacg > 2:57050/1‑140 (MQ=255) ttCAGTCCCCATGGCTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGacgacg < 1:471871/140‑1 (MQ=255) gTTTGTAACGCCAGCAACCGCCCCACTGTACGCTGAAAACCACCACGGAGTGGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAg < 2:415723/140‑1 (MQ=255) | GACGGAGATCGACCGTTTTCAGGATTTCCAGCCCGCCATTAAGCACGTCAACCTCCGGCATCGGTAACGCCAGAACGGTTTTCTTCAACGCCCGCGCATCCTGGCGCGCCGTTTCAGTCCCCATGGTTTGTAACGCCAGGAACCGCTCCACTGTACGCTGAAAATCATCACTGAGTTGCTGCTGAAATCCCGCCAGCACGTCCGGTTTTATCCCCGGCCACTCGTCACGAGCACTAAAACAAGGTGACGACGCCACGGTGACCAG > NC_000913/3544277‑3544541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |