Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,547,748	G→A	G397D (GGC→GAC)	gntT →	high‑affinity gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,547,748	0	G	A	100.0%	144.1 / NA	45	G397D (GGC→GAC)	gntT	high‑affinity gluconate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/28);  total (17/28)

CCGCAACCGTTGCGGCAATCACTGCGGGTGGTATCGCGGCACCGCTGATTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGA  >  NC_000913/3547616‑3547884                                                                                                                                     |                                                                                                                                         ccGCAACCGTTGCGGCAATCACTGCGGGTGGTATCGCGGCACCGCTGATTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTgg                                                                                                                                   <  1:153980/140‑1 (MQ=255) ccGCAACCGTTGCGGCAATCACTGCGGGTGGTATCGCGGCACCGCTGATTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTgg                                                                                                                                   <  1:560386/140‑1 (MQ=255)        cGTTGCGGCAATCACTGCGGGTGGTATCGCGGCACCGCTGATTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCa                                                                                                                            <  2:555308/140‑1 (MQ=255)         gTTGCGGCAATCACTGCGGGTGGTATCGCGGCACCGCTGATTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGCTAGTGTGATTTTTTCTCATGTGAACTATCCAGACTTCTGGCTGTTCaa                                                                                                                           >  2:226130/1‑140 (MQ=255)            gacagaaTCACTGCGAGTGGTAGCGAGGCACCGCTGAGTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAaga                                                                                                                        <  1:235674/135‑1 (MQ=255)            gCGGCAATCCCTGCGGGTGGTATCGCGGCTCCGCTGATTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAaga                                                                                                                        <  2:119405/140‑1 (MQ=255)            gCGGCAATCACTGCGTGTGGTAACGAGGCACCGCTGAATGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAaga                                                                                                                        <  1:262937/140‑1 (MQ=255)            gCGGCAATCACTGCGGGGGGTATCGCGGCACGGCTCATTCCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAaga                                                                                                                        <  2:196245/140‑1 (MQ=255)            gCGGCAATCACTGCGCGTGGTACCGCGGCGCCGCTGATTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAaga                                                                                                                        <  1:263960/140‑1 (MQ=255)            gCGGCAATCACTGCGAGTGGTAGCGAGGCGCCGCTGATTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAaga                                                                                                                        <  1:317123/140‑1 (MQ=255)                 aaTCACTGCGGGTGGTATCGCGGCACCGCTGATTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACt                                                                                                                   >  1:395347/1‑140 (MQ=255)                            gtggtATCGCGGCACCGCTGATTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCAAGTGAACGAGCCGCACTGCTGGCTGTACAAACAGTACTTTAAccctgtc                                                                                                        >  2:235674/1‑135 (MQ=255)                              ggtATCGCGGCACCGCTGATTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTAt                                                                                                      >  1:354466/1‑140 (MQ=255)                                      ggCACCGCTGATTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCgaga                                                                                              <  2:335835/140‑1 (MQ=255)                                          ccGCTGATTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGGGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGGTCAAAGAGTACATTCACCTGATTATCGGCGAGACCAt                                                                                          >  1:206004/1‑140 (MQ=255)                                           cGCTGATTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATc                                                                                         <  2:395347/140‑1 (MQ=255)                                            gCTGATTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCa                                                                                        >  2:399661/1‑140 (MQ=255)                                                        gacgGGTGTTAGCCCTGAGCTGATGCTTACTGCGGTTTGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGa                                                                            <  1:226130/140‑1 (MQ=255)                                                            ggTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCt                                                                        <  1:464509/140‑1 (MQ=255)                                                               gtTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGa                                                                     <  1:381259/140‑1 (MQ=255)                                                               gtTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGa                                                                     <  2:359930/140‑1 (MQ=255)                                                                 tAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGaaa                                                                   >  2:96751/1‑140 (MQ=255)                                                                          gCTGATGGTTATTGCAGTTGGTACCAGTAATGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTAtct                                                          <  1:255262/140‑1 (MQ=255)                                                                              aTGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGgt                                                      >  2:62795/1‑140 (MQ=255)                                                                              aTGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCCCGgt                                                      >  1:59531/1‑140 (MQ=255)                                                                                  ttATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGc                                                  <  1:399661/140‑1 (MQ=255)                                                                                   tATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCg                                                 <  1:91650/140‑1 (MQ=255)                                                                                   tATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCg                                                 <  2:591065/140‑1 (MQ=255)                                                                                   tATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCg                                                 >  1:471228/1‑140 (MQ=255)                                                                                   tATTGCGGTTGGTTCAGGTAGTGTGATTTTCTCTCATCTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCg                                                 <  1:241189/140‑1 (MQ=255)                                                                                       gCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCt                                             <  2:54053/140‑1 (MQ=255)                                                                                          gttggttCCGGTAGTGTCATTTTCTCTCATGCGAAAGATGCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGt                                          <  2:206004/140‑1 (MQ=255)                                                                                           ttggttCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTa                                         >  2:479377/1‑140 (MQ=255)                                                                                             ggttCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAgg                                       >  1:330182/1‑140 (MQ=255)                                                                                                       gtgtGATTTTCTCTCTTGTGAAGGATCCGGACTTCTGCCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTctgctg                             <  1:243895/140‑1 (MQ=255)                                                                                                       gtgtGATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTctgctg                             <  1:476037/140‑1 (MQ=255)                                                                                                         gtgATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAAGCATGATCACGGTGTGCGGTCTGGTAGGCTGTctgctgct                           >  2:181417/1‑140 (MQ=255)                                                                                                         gtgATTTTCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTctgctgct                           >  2:478631/1‑140 (MQ=255)                                                                                                               ttCTCTCATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAatat                     >  2:578832/1‑140 (MQ=255)                                                                                                                    tcATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGa                <  2:354466/140‑1 (MQ=255)                                                                                                                     cATGTGAACGATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGAt               <  2:69910/140‑1 (MQ=255)                                                                                                                              gATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAaca      <  1:478631/140‑1 (MQ=255)                                                                                                                              gATCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAaca      >  2:593610/1‑140 (MQ=255)                                                                                                                                tCCGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACact    >  1:641985/1‑140 (MQ=255)                                                                                                                                  cGGACTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACactga  <  1:630402/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         CCGCAACCGTTGCGGCAATCACTGCGGGTGGTATCGCGGCACCGCTGATTGCAACGACGGGTGTTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTGTTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGGTCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGATAACACTGA  >  NC_000913/3547616‑3547884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑