Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,563,647	T→C	intergenic (+129/+77)	glpD → / ← yzgL	aerobic glycerol 3‑phosphate dehydrogenase/putative uncharacterized protein YzgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,563,647	0	T	C	100.0%	123.0 / NA	39	intergenic (+129/+77)	glpD/yzgL	aerobic glycerol 3‑phosphate dehydrogenase/putative uncharacterized protein YzgL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/19);  total (20/19)

GCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCA  >  NC_000913/3563510‑3563779                                                                                                                                          |                                                                                                                                     gCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAg                                                                                                                                    >  2:556411/1‑140 (MQ=255)        aaaTTAACGTAAGGTGACCAGGCCAGATTTCTATCTGGCCTGAGACTGGTGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGAt                                                                                                                             <  2:180850/140‑1 (MQ=25)                gTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTCGCACGGGCTTGCACGCCAGATAAGACGtt                                                                                                                     >  1:222083/1‑140 (MQ=25)                    ggTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTATTGCAGGCCTGATAAGACGTTCACg                                                                                                                 >  1:317234/1‑140 (MQ=255)                        aTCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGc                                                                                                             >  2:371354/1‑140 (MQ=255)                          cAGGTCAGATTTCAATGGGGCCTGAGACTGACGACCAACTCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCAt                                                                                                           <  2:300948/140‑1 (MQ=21)                             gTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCg                                                                                                        >  1:373367/1‑140 (MQ=255)                             gTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCg                                                                                                        >  1:649965/1‑140 (MQ=255)                             gTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCg                                                                                                        >  1:413200/1‑140 (MQ=255)                                            ggCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACacacccc                                                                                         >  2:651390/1‑134 (MQ=255)                                            ggCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAgcgc                                                                                         >  2:463323/1‑140 (MQ=255)                                                   gaCTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGt                                                                                  >  1:97116/1‑140 (MQ=255)                                                     cTGATGACAAACACATAACTGCATGCTGCTCTACGCTGATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCa                                                                                <  1:295814/140‑1 (MQ=18)                                                        atgaCAAACACAAAAGTGCCTGGTCCGCTACGCTTATTAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCaaaa                                                                             <  1:291005/140‑1 (MQ=18)                                                                  caAAACTGCCTGATGCGCTACGGTTATCAGGCCTATGTGATTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTa                                                                   <  2:213239/140‑1 (MQ=255)                                                                  caAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTa                                                                   <  1:102588/140‑1 (MQ=255)                                                                       cTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGAGCACTTTGTCAAATATCTAACCTACtttt                                                              >  1:193447/1‑140 (MQ=255)                                                                             gATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTc                                                        >  1:42841/1‑140 (MQ=255)                                                                              aTGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCa                                                       <  1:463323/140‑1 (MQ=255)                                                                                           aTCAGGCCTACGTGGTTCATGCTATATATTGGATTAGCACGGTCTTGCCGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCa                                          <  2:193447/140‑1 (MQ=37)                                                                                                gCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAACTCTAACCCACTTTTTACTTCAAGGAATTACCGCAAAGcc                                     >  2:253209/1‑140 (MQ=25)                                                                                                gCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGcc                                     <  1:371354/140‑1 (MQ=255)                                                                                                   tACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCAAACGTACATTTTAATTGAGGGAATTACCGCAAAGCCCAc                                  >  2:218338/1‑140 (MQ=25)                                                                                                    tcGTGGTTTATGCAATACATTGCATTTGCATTGTCATGCGGGCCAGATTAGACGTTCACGGCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCCAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACg                                 <  2:222083/139‑1 (MQ=37)                                                                                                    aCGTGGTTTATGCAATATATTGCATTTGCATGGTCTTGCCGGCCAGATTAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACg                                 <  2:268570/140‑1 (MQ=255)                                                                                                    aCGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTCTTAATTCAGGGAATTACCGCAAAGCCCACg                                 <  2:413200/140‑1 (MQ=255)                                                                                                    aCGTGGTTTATGCAACATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACg                                 >  1:3182/1‑140 (MQ=255)                                                                                                     cGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACTTACTTTTCAATTCATGGAATTCCCGCAAAGCCCACGc                                >  1:268570/1‑140 (MQ=37)                                                                                                       tGGTTTATGCAATATATTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCca                              <  1:387516/140‑1 (MQ=255)                                                                                                                 aaTATATTGAATTTGCATGGTCTTGCAGGCCTGATCAGATGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCaa                    <  2:209335/140‑1 (MQ=255)                                                                                                                   tatatTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACg                  >  2:361654/1‑140 (MQ=255)                                                                                                                   tatatTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACg                  >  2:569904/1‑140 (MQ=255)                                                                                                                   tatatTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACg                  <  2:42841/140‑1 (MQ=255)                                                                                                                   tatatTGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACg                  >  1:647212/1‑140 (MQ=255)                                                                                                                        tGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACa             <  2:603782/140‑1 (MQ=255)                                                                                                                        tGAATTTGCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACa             >  1:150324/1‑140 (MQ=255)                                                                                                                               gCATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCa      >  1:468446/1‑140 (MQ=255)                                                                                                                                cATGGTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAg     <  2:3182/140‑1 (MQ=255)                                                                                                                                   ggTCTTGCCGGCCAGAATAGACATTCACGTCGCGTTCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCa  <  2:317234/140‑1 (MQ=255)                                                                                                                                   ggTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCa  <  2:373367/140‑1 (MQ=255)                                                                                                                                   ggTCTTGCAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCa  <  2:649965/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     GCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCA  >  NC_000913/3563510‑3563779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑