Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,565,249	T→C	M445V (ATG→GTG)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,565,249	0	T	C	100.0%	102.2 / NA	30	M445V (ATG→GTG)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/21);  total (9/21)

AGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATA  >  NC_000913/3565116‑3565384                                                                                                                                      |                                                                                                                                        aGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCATCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGAtt                                                                                                                                   >  2:550465/1‑140 (MQ=255)       tCGTCCTGCTCCTCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCTGCGTCACACCGCTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTg                                                                                                                             <  2:411382/140‑1 (MQ=255)       tCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTg                                                                                                                             <  1:487624/140‑1 (MQ=255)        cGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGc                                                                                                                            <  2:644673/140‑1 (MQ=255)        cGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGc                                                                                                                            <  1:354343/140‑1 (MQ=255)        cGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGc                                                                                                                            >  1:5900/1‑140 (MQ=255)               cACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCg                                                                                                                     >  2:99995/1‑140 (MQ=255)                 cGGCTGAAAGCGATGCGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGAt                                                                                                                   <  1:302982/140‑1 (MQ=255)                    cTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATaca                                                                                                                <  2:544505/140‑1 (MQ=255)                         aGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATACACCGtt                                                                                                           <  2:634435/140‑1 (MQ=255)                                ggTTCGCTACCGCCAACCAGCGTCGCGGCTTCACACCTTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTtg                                                                                                    <  1:319529/140‑1 (MQ=255)                                     gCTACCGCCAGCCAGCGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCt                                                                                               <  1:641743/140‑1 (MQ=255)                                     gCTACCGCCAGCCAGCGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCt                                                                                               <  1:637745/140‑1 (MQ=255)                                          cGCCAGCCAGCGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTACGAAACGCCGTTAGCTTTGTGGCTCacaa                                                                                          >  1:196131/1‑140 (MQ=255)                                           gccagccaGCGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCacaac                                                                                         <  1:119244/140‑1 (MQ=255)                                                ccagcGACGCGGCGTGACACCGGTGATGAGGTTGGTGGAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCACACAACAACCg                                                                                    <  1:281908/140‑1 (MQ=255)                                                ccagcGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCg                                                                                    <  2:526065/140‑1 (MQ=255)                                                    cGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCgccag                                                                                <  1:458435/140‑1 (MQ=255)                                                      aCGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCgccagcc                                                                              <  1:495957/140‑1 (MQ=255)                                                                      ttggtgacgttggtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACacg                                                              >  1:419344/1‑140 (MQ=255)                                                                        ggtgacgttggtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCCCCAGATTAGAGTGCAGTTCCGATACGCCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACacgac                                                            <  2:636371/140‑1 (MQ=255)                                                                        ggtgacgttggtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGACTCACAAGAACTGCGAGCCAGGCCATACGCACacgac                                                            >  1:215759/1‑140 (MQ=255)                                                                            acgttggtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCCCACAACACCCGCCAGCCAGGCCATACGCACACGACGAcc                                                        >  2:241674/1‑140 (MQ=255)                                                                                tggtgaAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTg                                                    >  1:585196/1‑140 (MQ=255)                                                                                                   gATTTTCGCAAAGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACg                                 <  1:63319/140‑1 (MQ=255)                                                                                                             aaGTCGGCAAACAACGATTGCACCACCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCcag                       <  1:550465/140‑1 (MQ=255)                                                                                                                            gATTGCACCACCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGtt        <  1:405573/140‑1 (MQ=255)                                                                                                                              ttGCACCACCAGATTAGCGTGCAGTTCCGATTCACGGTTCACTTTGTGCCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCg      <  2:215759/140‑1 (MQ=255)                                                                                                                              ttGCACCACCAGATTAGAGTGCCGTTCCGATTCACCGTTCACTTTGTGACTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCg      <  2:196131/140‑1 (MQ=255)                                                                                                                              ttGCACCACCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCg      >  2:349569/1‑140 (MQ=255)                                                                                                                                  accaccAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATa  <  2:585196/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        AGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCGTTGGTGACGTTGGTGAAACGACCCGGGAAGATTTTCGCAAAGTCGGCAAACAACGATTGCACCATCAGATTAGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATA  >  NC_000913/3565116‑3565384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑