Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,629,661	A→C	R314R (CGT→CGG)	yhiI ←	putative membrane fusion protein YhiI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,629,661	0	A	C	100.0%	82.8 / NA	25	R314R (CGT→CGG)	yhiI	putative membrane fusion protein YhiI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/13);  total (12/13)

ATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATACGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGC  >  NC_000913/3629530‑3629796                                                                                                                                    |                                                                                                                                        aTGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATCCGCGCttt                                                                                                                                 <  1:491668/140‑1 (MQ=255)   gCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATCCGCGCTTTGa                                                                                                                               <  2:42774/140‑1 (MQ=255)       cATTGCGGCAACCTCCCCACGATGTCGTCCGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATCCGCGCTTTGAcgcg                                                                                                                           <  1:204131/140‑1 (MQ=255)           gCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATCCGCGCTTTGACGCGGAAc                                                                                                                       >  2:346041/1‑140 (MQ=255)               cAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATCCGCGCTTTGACGCGGAACATCa                                                                                                                   >  1:15437/1‑140 (MQ=255)                                       ccACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATCCGCGCTTTAACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGttt                                                                                           >  1:295537/1‑140 (MQ=255)                                                ttcttcATTCACCCGCAACCACTCTACGCCCTGCACACCAGTTTTGACCTATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGttt                                                                                  <  2:187660/140‑1 (MQ=255)                                                          aCCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGaa                                                                        >  2:61918/1‑140 (MQ=255)                                                          aCCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGaa                                                                        >  2:355218/1‑140 (MQ=255)                                                          aCCCGCACCCACGCTACGCCCGACAAACCGGCTTTGACATATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGaa                                                                        <  2:284235/140‑1 (MQ=255)                                                          aCCCGCACCCACGCTACCCCCGGCAAACCGGTTTTTACAGATTCCAGACGCTGCTGGAGTAATTCCGGTGGGATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTAGACGGTTTTTGGCGTGaa                                                                        <  2:171860/140‑1 (MQ=255)                                                               cACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTggg                                                                   >  1:103371/1‑140 (MQ=255)                                                                          cGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCg                                                        <  2:329203/140‑1 (MQ=255)                                                                                   aaCCGGTTTTGACATATCCCAGATGCTGCTGTAGTCATTACGGTGGGACCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTg                                               <  2:172813/140‑1 (MQ=255)                                                                                    aCCGGTTTTGACATATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTATTTGGCGGGAATTGTGCGACATTGGCGACAAAACTGa                                              >  1:172813/1‑140 (MQ=255)                                                                                     ccGGTTTTGACATATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGAt                                             <  1:638939/140‑1 (MQ=255)                                                                                     ccGGTTTTGACATATTCCAGATGCTGCTGGAATCATTCCTGTGGGATCAGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGAt                                             <  2:295537/140‑1 (MQ=255)                                                                                                            ctgctgGAGTAATTCCGGTGGGATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTg                      >  1:629480/1‑140 (MQ=255)                                                                                                             tgctgGAGTAATTCCGGTGGGATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTgg                     >  1:399878/1‑140 (MQ=255)                                                                                                              gctgGAGTAATTCCGGTGGGATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAAATGAGGGGTGCAGGCATACGCAGATCTGgc                    >  1:170318/1‑140 (MQ=255)                                                                                                                 ggAGTAATTCCGGTGGGATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGcgcg                 <  1:61918/140‑1 (MQ=255)                                                                                                                     tAATTCCGGTGGGATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCAt             <  1:355218/140‑1 (MQ=255)                                                                                                                             gTGGGATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATc     >  1:543433/1‑140 (MQ=255)                                                                                                                             gTGGCATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGTCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATc     >  2:466623/1‑140 (MQ=255)                                                                                                                                ggATCCGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGc  <  2:15437/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        ATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTTGACATATTCCAGATGCTGCTGGAGTAATTCCGGTGGGATACGCGCTTTGACGCGGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATCAGC  >  NC_000913/3629530‑3629796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑