Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,664,804	A→G	intergenic (‑186/‑60)	gadW ← / → gadY	DNA‑binding transcriptional dual regulator GadW/small regulatory RNA GadY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,664,804	0	A	G	100.0%	226.4 / NA	65	intergenic (‑186/‑60)	gadW/gadY	DNA‑binding transcriptional dual regulator GadW/small regulatory RNA GadY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/27);  total (38/27)

ATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTATAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTA  >  NC_000913/3664668‑3664935                                                                                                                                         |                                                                                                                                    atgaaatgaTGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAATTAAATGCAGCAGGAGTATATTGTCGCACAGCGTGTAg                                                                                                                                  >  1:205979/1‑140 (MQ=255) atgaaatgaTGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAg                                                                                                                                  >  2:432981/1‑140 (MQ=255) atgaaatgaTGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAg                                                                                                                                  >  1:89887/1‑140 (MQ=255)  tgaaatgaTGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGc                                                                                                                                 >  1:477485/1‑140 (MQ=255)  tgaaatgaTGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATATTCGGAGAGCGTGTAGc                                                                                                                                 >  1:418009/1‑140 (MQ=255)  tgaaatgaTGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACCAAAATATTTTCGCACAGCGTGTAGc                                                                                                                                 >  1:302230/1‑140 (MQ=255)    aaatgaTGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAGATGCAGCACGAATATATTTTCGCACAGCGTGTAGCtt                                                                                                                               >  1:236403/1‑140 (MQ=255)    aaatgaTGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCtt                                                                                                                               >  2:388305/1‑140 (MQ=255)      atgatgCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAAGGCAGCACCAATATATTTTCGCCCAGCGTGTAGCTTAt                                                                                                                             >  1:199930/1‑140 (MQ=255)       tgatgCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATg                                                                                                                            <  2:599661/140‑1 (MQ=255)         atgCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGtt                                                                                                                          >  1:381759/1‑140 (MQ=255)         atgCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGtt                                                                                                                          >  2:567063/1‑140 (MQ=255)           gCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTta                                                                                                                        >  1:357664/1‑140 (MQ=255)           gCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTta                                                                                                                        >  2:90931/1‑140 (MQ=255)                aCTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATaaaa                                                                                                                   >  1:81519/1‑140 (MQ=255)                        ttAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCt                                                                                                           <  1:89788/140‑1 (MQ=255)                         tAGCGCAAAAACTGCCAAATTTTGTAGGACCCAAATTTATCCACAAAGCAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTg                                                                                                          <  2:239833/140‑1 (MQ=255)                            cgcAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATc                                                                                                       >  1:649249/1‑140 (MQ=255)                              cAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCtt                                                                                                     >  1:388589/1‑140 (MQ=255)                                         aGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTaa                                                                                          <  2:546887/140‑1 (MQ=255)                                            tttGGTAGGACCAAAGTTTATCCACTAAGTAGTTTTGGATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAg                                                                                       <  1:242163/140‑1 (MQ=255)                                              tGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGtt                                                                                     <  2:357664/140‑1 (MQ=255)                                                     aCCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATTGCTGATCTTATTTCCAGTAAAAGTTATATTTa                                                                              >  2:87036/1‑140 (MQ=255)                                                      ccAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTaa                                                                             >  2:388592/1‑140 (MQ=255)                                                                    caAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGcac                                                               >  2:652464/1‑140 (MQ=255)                                                                     aaaGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGcaca                                                              >  1:410901/1‑140 (MQ=255)                                                                      aaGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGc                                                                 <  2:551459/136‑1 (MQ=255)                                                                      aaGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGc                                                                 >  1:551459/1‑136 (MQ=255)                                                                             tttGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTcc                                                      <  2:388589/140‑1 (MQ=255)                                                                             tttGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGACAGCACAAAGTTTcc                                                      <  2:410901/140‑1 (MQ=255)                                                                                 cATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTg                                                  >  1:543361/1‑140 (MQ=255)                                                                                     aCAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCaa                                              <  1:567063/140‑1 (MQ=255)                                                                                       aGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACa                                            >  2:66067/1‑140 (MQ=255)                                                                                       aGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACa                                            <  2:81519/140‑1 (MQ=255)                                                                                       aGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACa                                            <  2:51106/140‑1 (MQ=255)                                                                                       aGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACa                                            <  2:89887/140‑1 (MQ=255)                                                                                         ttAATTGATAATTTGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGTTGATCTTATTTCCAGTAAATGTTATATATAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAgg                                          <  2:418009/140‑1 (MQ=255)                                                                                         ttAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATTGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAgg                                          <  1:87036/140‑1 (MQ=255)                                                                                         ttAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAgg                                          <  2:477485/140‑1 (MQ=255)                                                                                           aaTTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAAGTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGa                                        <  2:612849/140‑1 (MQ=255)                                                                                            aTTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAg                                       <  2:155830/140‑1 (MQ=255)                                                                                            aTTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAg                                       <  1:432981/140‑1 (MQ=255)                                                                                                ataataAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGtt                                   >  2:141941/1‑140 (MQ=255)                                                                                                ataataAGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTGACTGAGAACACAAAGTTTCCCGTGCCAACAGGGAGTGtt                                   >  2:238045/1‑140 (MQ=255)                                                                                                 taataaGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTTCTGAGAGGACAAAGTTCCCCGTGCCAACAGGGAGTGTta                                  >  2:317991/1‑140 (MQ=255)                                                                                                 taataaGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTta                                  >  2:520861/1‑140 (MQ=255)                                                                                                      aGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACg                             <  2:649249/140‑1 (MQ=255)                                                                                                      aGTAAATGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACg                             >  1:623490/1‑140 (MQ=255)                                                                                                           aTGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTATTGAGAGCAAAAACTTTCCCGTGCCACCAGGAAGTGTTATAACGGTtta                        >  2:280564/1‑140 (MQ=255)                                                                                                           aTGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTtta                        >  2:381585/1‑140 (MQ=255)                                                                                                           aTGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTtta                        >  2:426330/1‑140 (MQ=255)                                                                                                           aTGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTtta                        >  2:442898/1‑140 (MQ=255)                                                                                                           aTGCAGCACGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTtta                        <  2:479385/140‑1 (MQ=255)                                                                                                           aTGCAGCACGAATATATATTCGAACAGCGTGAAGCTTATATTTATAAAGAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTtta                        <  2:205979/140‑1 (MQ=255)                                                                                                                 caCGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTc                  >  2:99072/1‑140 (MQ=255)                                                                                                                 caCGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTc                  >  2:357714/1‑140 (MQ=255)                                                                                                                  aCGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCt                 >  2:159447/1‑140 (MQ=255)                                                                                                                  aCGAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTCCCCTTGCTAGCAGGGACTGTTATAACGGTTTATTAGTCt                 >  2:246253/1‑140 (MQ=255)                                                                                                                    gAATATATTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTgg               <  2:623490/140‑1 (MQ=255)                                                                                                                    gAATATATTTTCGCACAGCGTGTAGCTTAGGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAGCAGGGAGTGTTATAACGGTTTATTAGTCTgg               <  1:246253/140‑1 (MQ=255)                                                                                                                      atatatTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGgag             >  2:623887/1‑140 (MQ=255)                                                                                                                         tatTTTCGGTCAGCGTTTAGCTTATGTTTATAAAACAATTGCTGATCTTATTTCCAGTAAAAGTTATATTGAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACg          <  2:302230/140‑1 (MQ=255)                                                                                                                         tatTTTCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCTCAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACg          <  1:141941/140‑1 (MQ=255)                                                                                                                           ttttCGCACAGCGTGTATCTTATGTTTATAATAAATTGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGc        <  2:236403/140‑1 (MQ=255)                                                                                                                           ttttCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGc        >  2:352497/1‑140 (MQ=255)                                                                                                                           ttttCGCACAGCGTGCAGCTTACGTATATAAAAAAATAGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGc        <  1:317991/140‑1 (MQ=255)                                                                                                                              tCGCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGa     <  1:66067/140‑1 (MQ=255)                                                                                                                                gCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCGCAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACt   <  1:90931/140‑1 (MQ=255)                                                                                                                                gCACAGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGAGTTATAACGGTTAATTAGTCTGGAGACGGCAGACt   >  2:226436/1‑140 (MQ=255)                                                                                                                                 cacaGCGTGTAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAATGTTATAGCGGATTATTAGTCAGGAGACGGCAGACTa  >  1:233130/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    ATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTATAGCTTATGTTTATAAAAAAATGGCTGATCTTATTTCCAGTAAAAGTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTA  >  NC_000913/3664668‑3664935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑