Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,670,288	T→C	L233L (TTG→CTG)	treF →	cytoplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,670,288	0	T	C	100.0%	82.6 / NA	26	L233L (TTG→CTG)	treF	cytoplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/18);  total (8/18)

CGGGAAGATTTGCTGAAATGCATGGCCGATAACTTCGCCTGGATGATCGAAAACTACGGTCACATCCCCAACGGCAACCGCACCTATTATTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGTTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTCTGGATGGACGGTGCAGAATCGTTAATCCCTAATCAGGCCTATCGCCATGTTGTGCGGATGCCGGACG  >  NC_000913/3670156‑3670423                                                                                                                                     |                                                                                                                                        cGGGAAGATTTGCTACAATGCATGGCCGACATCTTCGCCTGGATGATCGAAAACTACGGTCACATCCCCAACGGCAACCGCACCTATTATTTGAGCCGCTCGCAACCCCCGGTTTTTGCGCTGATGGTGGAGCTGTTTga                                                                                                                                  <  1:176051/140‑1 (MQ=255)  gggAAGATTTGCTGAAATGCATTGCCGATTACTTCGCTTGGATGATCGAAAACTACGGTCACATCCCCAACGGCAACCGCACCTATTATTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTgaa                                                                                                                                 <  1:214606/140‑1 (MQ=255)      aGATTTGCTGAAATGCATGGCCGATAACTTCGCCTGGATGATCGAAAACTACGGTCACATCCCCAACGGCAACCGCACCTATTATTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGaagaag                                                                                                                             <  1:632352/140‑1 (MQ=255)            gCTGAAATGCATGGCCGATAACTTCGCCTGGATGATCGAAAACTACGGTCACATCCCCAACGGCAACCGCACCTATTATTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGgtg                                                                                                                       >  1:149306/1‑140 (MQ=255)                  aTGCATGGCCGATAACTTCGCCTGGATGATCGAAAACTACGGTCACATCCCCAACGGCAACCGCACCTATTATTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTAcgcg                                                                                                                 >  2:573059/1‑140 (MQ=255)                       tGGCCGATAACTTCGTCTGGATCATTGAAAACTACGGTCACATCCCCAACGGCAACCGCACCTATTATTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTgcg                                                                                                            <  1:248456/140‑1 (MQ=255)                            gATAACTTCGCCTGGATGATCGAAAACTACGGTCACATCCCCAACGGCAACCGCACCTATTATTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGcgccg                                                                                                       >  2:244780/1‑140 (MQ=255)                                aCTTCGCCTGGATGATCGAAAACTACGGTCACATCCCCAACGGCAACCGCACCTATTATTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTAt                                                                                                   <  2:554702/140‑1 (MQ=255)                                       cTGGATGATCGAAAACTACGGTCACATCCCCAACGGCAACCGCACCTATTATTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGacc                                                                                            <  1:443797/140‑1 (MQ=255)                                                         cGGTCACATCCCCAACGGCAACCGCACCTATTATTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATg                                                                          >  1:117500/1‑140 (MQ=255)                                                           gTCACATCCCCAACGGCAACCGCACCTATTATTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGcc                                                                        <  1:136608/140‑1 (MQ=255)                                                                      aaCGGCAACCGCACCTATTATTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTCtggatgga                                                             <  2:149306/140‑1 (MQ=255)                                                                      aaCGGCAACCGCACCTATTATTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTCtggatgga                                                             <  1:157503/140‑1 (MQ=255)                                                                                     tattatTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTCTGGATGGACGGTGCAGAATCGtt                                              <  1:637550/140‑1 (MQ=255)                                                                                     tattatTTGAGCCGCGCGCAACCACCGGTCTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTCTGGATGGACGGTGCAGAATCGtt                                              <  1:178991/140‑1 (MQ=255)                                                                                          tttGAGCCGCTCGCAGCCACCGTTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTCTGGATGGACGGTGCAGAATCGTTAATcc                                         <  1:300126/140‑1 (MQ=255)                                                                                          tttGAGCCGCTCGCAACCACCGGTTTTTGGGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTCTGGATGGACGGTGCAGAATCGTTAATcc                                         <  1:244780/140‑1 (MQ=255)                                                                                          tttGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTCTGGATGGACGGTGCAGAATCGTTAATcc                                         <  2:97653/140‑1 (MQ=255)                                                                                              aGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCGTTCTGGATGGACGGTGCCGAATCGTTAATCCCTaa                                     >  2:292804/1‑140 (MQ=255)                                                                                                   cTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTGTGGATGGACGGAGCAGAATCGTTAATCCCTAATCAgg                                >  1:211579/1‑140 (MQ=255)                                                                                                   cTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTCTGGATGGACGGTGCAGAATCGtt                                              >  1:394275/1‑126 (MQ=255)                                                                                                   cTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTCTGGATGGACGGTGCAGAATCGtt                                              <  2:394275/126‑1 (MQ=255)                                                                                                     cGCAACCACCGGTTTTTGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTCTGGATGGACGGTGCAGAATCGTTAATCCCTAATCAGGcc                              <  1:573059/140‑1 (MQ=255)                                                                                                                gattttGCGCTGATGGTGGAGCTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTCTAGATGGACGTTGCAGAATCGTTAATCGCTAAGCAGGCCTATCGCCAtgt                   >  2:253604/3‑140 (MQ=255)                                                                                                                     tGCGCTGATGGTGGAGCAGTTTAAAGAAGATCGTGGACGAGGTGCGCGACGCTATCTCGACCACCTTAAAATGGAATATGCCTTCTGGATGGACGGTGCAGAATCGTTAATCCCTAATCAGGCCTATCGCCATGTTGTGc              <  2:211579/140‑1 (MQ=255)                                                                                                                                 ggAGCTGTTTGAAGATGATGGTCTACGCGCTGCGCGCTGCTATCTCGACCACCTTAAAATGGAATATGCCTTCTGGATGGACGGTGCAGAATCGTTAATCCCTAATCAGGCCTATCGCCATGTTGTGCGGATGCcggacg  <  1:253604/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        CGGGAAGATTTGCTGAAATGCATGGCCGATAACTTCGCCTGGATGATCGAAAACTACGGTCACATCCCCAACGGCAACCGCACCTATTATTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGTTGTTTGAAGAAGATGGTGTACGCGGTGCGCGCCGCTATCTCGACCACCTTAAAATGGAATATGCCTTCTGGATGGACGGTGCAGAATCGTTAATCCCTAATCAGGCCTATCGCCATGTTGTGCGGATGCCGGACG  >  NC_000913/3670156‑3670423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑