Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,673,175	G→A	R254R (CGG→CGA)	yhjC →	putative DNA‑binding transcriptional regulator YhjC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,673,175	0	G	A	100.0%	113.3 / NA	37	R254R (CGG→CGA)	yhjC	putative DNA‑binding transcriptional regulator YhjC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/18);  total (19/18)

CGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGGCTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAACCTGTTTATGCAGTGGCTGGCTGGCGTAATGAAAGAGTACCTGG  >  NC_000913/3673043‑3673308                                                                                                                                     |                                                                                                                                      cGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTg                                                                                                                                <  2:580056/140‑1 (MQ=255)  gTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGa                                                                                                                               >  1:408793/1‑140 (MQ=255)   tCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGaa                                                                                                                              >  2:439675/1‑140 (MQ=255)     ctgtgtTTTAAGTCTTGCGGCATGTAGACGGTAAACACTAGCAAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGt                                                                                                                            <  1:248363/138‑1 (MQ=255)         ggTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTa                                                                                                                        <  2:475541/140‑1 (MQ=255)          gTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTAc                                                                                                                       >  2:528254/1‑140 (MQ=255)              aaGTCTGGCGGCATGTTGACGGTAACCAGTAGCGAAATCTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTgg                                                                                                                   <  1:294209/140‑1 (MQ=255)                    ggcggcATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGCCGATTTATTGAAGGATTACCTGGCTACCg                                                                                                             >  1:328085/1‑140 (MQ=255)                           tGTTGAGGGTAAAGAGTAGCAAAAACTCTCTCCCCGCCGGTCTTGCCGGTTTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAg                                                                                                      <  2:634500/140‑1 (MQ=255)                             ttGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGcc                                                                                                    >  2:46936/1‑140 (MQ=255)                                            aGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCt                                                                                     >  1:148300/1‑140 (MQ=255)                                            aGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCt                                                                                     <  1:46936/140‑1 (MQ=255)                                             gCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTg                                                                                    >  1:398827/1‑140 (MQ=255)                                                aaaaCTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGtt                                                                                 >  1:24570/1‑140 (MQ=255)                                                       tctcACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTACTACCTGGCAACCGTGCAGAGCCGCTGTCCCGTTCGCTGGTTTATCCGc                                                                          >  2:188354/1‑140 (MQ=255)                                                       tctcACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTAATACCTGGCCACCGTGCGAAGCCGCTGTCCCTTTCGCTGGATTATCCGc                                                                          >  2:318838/1‑140 (MQ=255)                                                            ccgccgGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGt                                                                     >  2:638792/1‑140 (MQ=255)                                                              gccgGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCg                                                                   <  2:395123/140‑1 (MQ=255)                                                                          ggTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCg                                                       <  1:377540/140‑1 (MQ=255)                                                                          ggTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTCCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCg                                                       <  2:449423/140‑1 (MQ=255)                                                                                   attattCAGATCCCGTGCATTGACGAGCGTGAAGCCCGGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTaaa                                              <  1:318838/140‑1 (MQ=255)                                                                                    ttattCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAAc                                             <  1:638792/140‑1 (MQ=255)                                                                                     tattCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAAcc                                            >  1:86695/1‑140 (MQ=255)                                                                                             tCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAACCTGTTTATg                                    <  2:127903/140‑1 (MQ=255)                                                                                             tCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAACCTGTTTATg                                    <  1:127988/140‑1 (MQ=255)                                                                                                 gcgcATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAACCTGTTTATGCAGt                                >  2:620263/1‑140 (MQ=255)                                                                                                 gcgcATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGCAGCTTTCCCGCCGTGTAAACCTGTTTATGCAGt                                >  2:295713/1‑140 (MQ=255)                                                                                                  cgcATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAACCTGTTTATGCAGtg                               >  1:28889/1‑140 (MQ=255)                                                                                                  cgcATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAACCTGTTTATGCAGtg                               >  2:69737/1‑140 (MQ=255)                                                                                                       tGCCGTGCGCGAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAACCTGTTTATGCAGtggctgg                          <  1:570555/140‑1 (MQ=255)                                                                                                             gcgcGAAGCCCTGCGTGTCGGGCGACTTATTGAAGCATTCCCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAACCTGTTTATGCAGTGGCTGGCTGGCg                    <  2:237090/140‑1 (MQ=255)                                                                                                               gcgAAGCCCTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAGACCTGTTTATGCAGTGGCTGGCTGGCGTa                  >  1:294644/1‑140 (MQ=255)                                                                                                                      cttGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTTCCGGCGTGTAAACCTGTTTATGCAGTGGCTGGCTGGCGTAATGAAag           <  2:428462/138‑1 (MQ=255)                                                                                                                      ccTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAACCTGTTTATGCAGTGGCTGGCTGGCGTAATGAAag           <  1:69737/140‑1 (MQ=255)                                                                                                                      ccTGCGTGCCGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAACCTGTTTATGCAGTGGCTGGCTGGCGTAATGAAag           <  1:485397/140‑1 (MQ=255)                                                                                                                      ccTGCGTGCCGGGCGAAATATTGAAGTATTATCTGGCTAGCGTGCCGACCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAACCTGTTTATGCAGTGGCTGGCTGGCGTAATGAAag           <  2:296816/140‑1 (MQ=255)                                                                                                                           gTGCCGGGCGACTTATTCAAGTATTACCTGGATACGGTGCCGAGCCGCCCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAACCTGTTTATGCAGTGGCTGGCTGGCGTAATGAAAGAGTAc      <  2:224821/140‑1 (MQ=255)                                                                                                                               cGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAACCTGTTTATGCAGTGGCTTGCTGGCGTAGTGAAAGAGTACCTgg  >  1:272242/1‑140 (MQ=255)                                                                                                                               cGGGCGACTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAACCTGTTTATGCAGTGGCTGGCTGGCGTAATGAAAGAGTACCTgg  >  2:66973/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      CGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGATTATTCAGATCCCGCGCATTGCCGTGCGCGAAGCCCTGCGTGCCGGGCGGCTTATTGAAGTATTACCTGGCTACCGTGCCGAGCCGCTCTCCCTTTCGCTGGTTTATCCGCAGCGTCGGGAGCTTTCCCGGCGTGTAAACCTGTTTATGCAGTGGCTGGCTGGCGTAATGAAAGAGTACCTGG  >  NC_000913/3673043‑3673308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑