Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,757,729	A→G	R33R (CGT→CGC)	yiaY ←	L‑threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,757,729	0	A	G	100.0%	140.4 / NA	42	R33R (CGT→CGC)	yiaY	L‑threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/19);  total (23/19)

CGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCA  >  NC_000913/3757593‑3757861                                                                                                                                         |                                                                                                                                     cggcgACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCgg                                                                                                                                   >  2:104931/1‑140 (MQ=255)                tggtggGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCt                                                                                                                    >  2:620562/1‑140 (MQ=255)                 ggtggGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTg                                                                                                                   <  1:86169/140‑1 (MQ=255)                 ggtggGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTg                                                                                                                   >  2:86169/1‑140 (MQ=255)                 gggggggTTAGGTTGGGTGCCATCATAAATAACGATAAAAATTTTGCTTTCTTCCAGTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTg                                                                                                                   <  2:133707/137‑1 (MQ=255)                   gggggTTGGGTTGGGTGCCATCATAAAAAACGCTAAAAATTTTGCTTTCTTCCATTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGcc                                                                                                                 <  2:464987/139‑1 (MQ=255)                         tAGGTTGGGTGCCATAATAAATAACGCAATAAATATTCCGTTCTTCCAGTGTTTTTTACACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCata                                                                                                           <  1:211679/140‑1 (MQ=255)                            gTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTc                                                                                                        >  1:377684/1‑140 (MQ=255)                                ggTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTg                                                                                                    >  1:412106/1‑140 (MQ=255)                                ggTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTg                                                                                                    >  1:56107/1‑140 (MQ=255)                                ggTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTg                                                                                                    >  1:337668/1‑140 (MQ=255)                                   gCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCAt                                                                                                 <  1:587642/140‑1 (MQ=255)                                                 cGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCa                                                                                   >  1:651406/1‑140 (MQ=255)                                                  gCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAg                                                                                  >  2:415561/1‑140 (MQ=255)                                                    aaaaaaTATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAgcg                                                                                <  1:415561/139‑1 (MQ=255)                                                             tGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATGATAGTCAATGCATCAGGCAATGAATCAGCGCCGATGACa                                                                       >  2:169990/1‑140 (MQ=255)                                                               cGTTCTTCCAGTGTTATTTCCCTGTCGCCCGCCATACATAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACAtt                                                                     <  1:178009/140‑1 (MQ=255)                                                                  tcttcCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGGCATCATATACATGGCAGCAGTCAATAAATCGGCGCCGATGACATTcac                                                                  >  2:178009/1‑140 (MQ=255)                                                                       cgttgttttttGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAg                                                             <  2:412106/134‑1 (MQ=255)                                                                       cAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAg                                                             <  2:56107/140‑1 (MQ=255)                                                                               tttGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGAGGACATTCACAGAAGGAATAAAg                                                     >  1:299871/1‑140 (MQ=255)                                                                                       tCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGa                                             >  1:164395/1‑140 (MQ=255)                                                                                        cgcccgccATACCTACTTTCGTTAACATAATGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGTATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGaa                                            <  1:278164/140‑1 (MQ=255)                                                                                          cccgccATACCTAATTTTGTTATCATATTGTTAGTTACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGc                                          <  2:258269/140‑1 (MQ=255)                                                                                          cccgccATACCTAATTCCGTTACCATATTGTAAGTGACAATTAAGGTGTGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGc                                          <  2:299871/140‑1 (MQ=255)                                                                                           ccgccATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCAGATGACACTCATAGCAGGAATCAAGAACGTTGAAGCt                                         >  1:312914/1‑140 (MQ=255)                                                                                            cgccATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTg                                        <  2:651406/140‑1 (MQ=255)                                                                                               cATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCa                                     <  2:160530/140‑1 (MQ=255)                                                                                                 tACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATa                                   <  1:104931/140‑1 (MQ=255)                                                                                                  aCCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATaa                                  >  1:531745/1‑140 (MQ=255)                                                                                                     tAATTTCGTTAACATCTTGTCAGTGACAAATAAGGTGGGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAacac                               <  1:319605/140‑1 (MQ=255)                                                                                                     tAATTTCGTTAACATATTGTCAGTGGCAATTAAGGTGCGGGTAAATCCTTAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCGGCCATAacac                               <  2:585086/140‑1 (MQ=255)                                                                                                           cGTTAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCt                         >  1:37523/1‑140 (MQ=255)                                                                                                             ttAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAacac                               <  1:649014/132‑1 (MQ=255)                                                                                                             ttAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAacac                               >  2:649014/1‑132 (MQ=255)                                                                                                             ttAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGa                       >  1:580968/1‑140 (MQ=255)                                                                                                             ttAACATATTGTCAGTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGa                       <  2:83711/140‑1 (MQ=255)                                                                                                                           gTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAgg         >  1:109965/1‑140 (MQ=255)                                                                                                                           gTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAgg         >  2:28719/1‑140 (MQ=255)                                                                                                                           gTGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAAGAAAGAACGGTGAAGCTGCCATAACAAACTCTTGAAAGTGTAAAAAAgg         >  2:258457/1‑140 (MQ=255)                                                                                                                            tGACAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAggc        >  1:347333/1‑139 (MQ=255)                                                                                                                               cAATTAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAgggcgg     <  2:531745/140‑1 (MQ=255)                                                                                                                                  ttAAGGTGCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCa  >  2:152689/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     CGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCA  >  NC_000913/3757593‑3757861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑