Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,784,960	A→T	I19N (ATC→AAC)	yibN ←	putative sulfurtransferase YibN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,784,960	0	A	T	97.4%	128.3 / NA	39	I19N (ATC→AAC)	yibN	putative sulfurtransferase YibN
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (16/22);  total (17/22)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGG  >  NC_000913/3784823‑3785081                                                                                                                                          |                                                                                                                          aGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTc                                                                                                                         >  2:528290/1‑140 (MQ=255)     ttcaCGTTGACGTAAATCCACAACCCCAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATCACCTTCACTTACGAGGTAAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAgg                                                                                                                     <  2:630723/139‑1 (MQ=255)     atcaCGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAgg                                                                                                                     >  2:21521/1‑140 (MQ=255)       caCGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCg                                                                                                                   <  1:21521/140‑1 (MQ=255)          gCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATa                                                                                                                <  2:519888/140‑1 (MQ=255)          gCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATa                                                                                                                <  1:513173/140‑1 (MQ=255)           cTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATAc                                                                                                               <  1:642155/140‑1 (MQ=255)              aCGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACGAGAACCGGCACGAGGAACGCGATCCAGGCGATACTCa                                                                                                            >  1:175489/1‑140 (MQ=255)                        acaACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCAAAAGAACCGGCACGAGTAACGCGTTCCAGGCCATACTCAGTATGGGATg                                                                                                  >  2:240520/1‑140 (MQ=255)                          aaCCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGAc                                                                                                <  1:626386/140‑1 (MQ=255)                               caGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACGGCCACCAGTAACTCGTACCAGGCGAGACTCCGTATGGGATGACGGCca                                                                                           >  2:202610/1‑140 (MQ=255)                                              tgATCAGACGCGTAGCTTCACCGCGCGTATTCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAAtt                                                                            <  1:240520/140‑1 (MQ=255)                                                  cAGACGCGTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCtt                                                                        >  2:641514/1‑140 (MQ=255)                                                  cAGACGCGTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCtt                                                                        >  1:458175/1‑140 (MQ=255)                                                      cgcgTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCAt                                                                    <  2:443809/140‑1 (MQ=255)                                                       gcgTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATg                                                                   <  1:528290/140‑1 (MQ=255)                                                        cgTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATgg                                                                  <  1:357287/140‑1 (MQ=255)                                                                   cacGAGTAATCACCTACACTTTTGAGGTCGGGCTCTTGAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTaacaac                                                       <  1:292042/140‑1 (MQ=255)                                                                       aGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGAAGACGGCCAGCAAATGGGATAATTTCTTGCATAGGGGGTAACAACTccc                                                   >  2:306666/1‑140 (MQ=255)                                                                        gTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCg                                                  <  2:43269/140‑1 (MQ=255)                                                                             ccccTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTa                                             <  1:625741/138‑1 (MQ=255)                                                                                        tCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTaaaaa                                  >  2:458199/1‑140 (MQ=255)                                                                                                 gtctctAAAACGTATTCACAAAACCCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGaa                         <  2:458175/138‑1 (MQ=255)                                                                                                 ggCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGaa                         <  1:554898/140‑1 (MQ=255)                                                                                                 ggCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGaa                         <  1:1185/140‑1 (MQ=255)                                                                                                 ggCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGaa                         <  2:403173/140‑1 (MQ=255)                                                                                                 ggCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGaa                         <  1:641514/140‑1 (MQ=255)                                                                                                 ggCTCTTAAACGTAGCCACAAGTACTGCCACCAGTAATGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGaa                         <  1:202610/140‑1 (MQ=255)                                                                                                  gCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGaaa                        >  2:366258/1‑140 (MQ=255)                                                                                                  gCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGaaa                        >  1:465843/1‑140 (MQ=255)                                                                                                             tAGTCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCt             >  2:119388/1‑140 (MQ=255)                                                                                                                tCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTgcg          >  2:334307/1‑140 (MQ=255)                                                                                                                tCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTgcg          <  1:334307/140‑1 (MQ=255)                                                                                                                tCACAAGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCAT‑GGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTgcgc         >  1:40470/1‑140 (MQ=255)                                                                                                                    aaGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGt      >  2:380807/1‑140 (MQ=255)                                                                                                                    aaGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGt      >  2:485639/1‑140 (MQ=255)                                                                                                                     aGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGcgcg        <  2:467170/137‑1 (MQ=255)                                                                                                                     aGAACCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGcgcg        >  1:467170/1‑137 (MQ=255)                                                                                                                       aaCCGCCACCAGTAACGCGTTCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCAT‑GGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGTGTATACCTGCGCGGTGCgg  <  2:40470/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                          AGTCATCACGCTGACGTAAATCCACAACCACAGCGTCTTCTTTGTTGATCAGACGCGTAGCTTCACCACGAGTAATCACCTTCACTTTCGAGGTCAGGCTCTTAAACGTAGTCACAAGAACCGCCACCAGTAACGCGATCCAGGCGATACTCAGTATGGGATGACGGCCAACAAATTGCATAATTTCTTGCATGGGGGGTAACAACTCCCGACGTAGTGATTAAAAAAACCAGGAAAGGAGTATACCTGCGCGGTGCGG  >  NC_000913/3784823‑3785081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑