Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	388,948	A→G	R260R (CGT→CGC)	hemB ←	porphobilinogen synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	388,948	0	A	G	100.0%	66.9 / NA	20	R260R (CGT→CGC)	hemB	porphobilinogen synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/12);  total (8/12)

CACCCGCACGCTTAATCGAACCTAAGCTTTCGAGCACGACTTTCTCTTCATCTATAGCACCCGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCACGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAATCGCCTCACGACGGTTCATTGGGTTCATCTGATAGCTT  >  NC_000913/388812‑389077                                                                                                                                         |                                                                                                                                  cccccgcacGCTTAATCCAACCCAAGCTTTCTACCCCGAATTTCTCTTTATCTATAGCACCCGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCgcgc                                                                                                                                <  2:288264/138‑1 (MQ=255)           cTTAATCGAACCTAAGCTTTCGAGCACGACTTTCTCTTCATCTATAGCACCCGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCGCGCAGCTcacgca                                                                                                                      <  2:456917/140‑1 (MQ=255)           cTTAATCGAACCTAACCTTTCGTGCGCGATTTTCTCTCCATCTATAGCCCCCGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCGCGCAGCTcacgca                                                                                                                      <  1:244408/140‑1 (MQ=255)                                         tttCTCTTCATCTATAGCACCCGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGTAGGTTTaa                                                                                        <  2:507879/140‑1 (MQ=255)                                                            cccGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGcc                                                                     >  1:387931/1‑140 (MQ=255)                                                             ccGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGccc                                                                    <  1:644044/140‑1 (MQ=255)                                                               gCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTg                                                                  >  2:395654/1‑140 (MQ=255)                                                                                      cGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCAc                                           >  1:376889/1‑140 (MQ=255)                                                                                       gCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACg                                          >  1:86929/1‑140 (MQ=255)                                                                                          tACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAAt                                       <  2:387931/140‑1 (MQ=255)                                                                                          tACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAAt                                       <  2:521826/140‑1 (MQ=255)                                                                                          tACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAAt                                       <  2:599400/140‑1 (MQ=255)                                                                                               aCCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAATCGCCt                                  >  2:486598/1‑140 (MQ=255)                                                                                               aCCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGATTCGTCTAGCAGTCATTCACGAATCGCCt                                  >  1:311394/1‑140 (MQ=255)                                                                                                   cTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAATCGCCTCacg                              <  1:376843/140‑1 (MQ=255)                                                                                                   cTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAATCGCCTCacg                              >  2:56920/1‑140 (MQ=255)                                                                                                   cTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGCTTAACCATCAGGCAGTCTGCGCCCGGGGCTTCCTCCGGCGGTGATTAACGAATCGCCTCacg                              >  1:175313/1‑140 (MQ=255)                                                                                                               gcgcCAATCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAATCGCCTCACGACGGTTCATTgg                  <  1:58157/140‑1 (MQ=255)                                                                                                                  ccAATCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAATCGCCTCACGACGGTTCATTGGGtt               <  1:486598/140‑1 (MQ=255)                                                                                                                     aTCGGCAATTCAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAATCGCCTCACGACGGTTCATTGGGTTCAt            <  2:84308/140‑1 (MQ=255)                                                                                                                               cAGTACGTTCGCGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAATCGCCTCACGACGGTTCATTGGGTTCATCTGATAGCtt  <  1:395654/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  CACCCGCACGCTTAATCGAACCTAAGCTTTCGAGCACGACTTTCTCTTCATCTATAGCACCCGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCACGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAATCGCCTCACGACGGTTCATTGGGTTCATCTGATAGCTT  >  NC_000913/388812‑389077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑