Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,917,862	G→A	A135V (GCC→GTC)	atpG ←	ATP synthase F1 complex subunit gamma

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,917,862	0	G	A	100.0%	111.7 / NA	36	A135V (GCC→GTC)	atpG	ATP synthase F1 complex subunit gamma
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/13);  total (23/13)

ATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGGCAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAGGTCTTCATTTCCGCCAGCAGTTTTTTGAACAGGTTAATGTTCA  >  NC_000913/3917730‑3917994                                                                                                                                     |                                                                                                                                     aTGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACaacaac                                                                                                                               >  2:4141/1‑140 (MQ=255)   ggTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACAt                                                                                                                             >  1:591322/1‑140 (MQ=255)     tgtTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCTTGCAACATCACTTTTACCGGACCGATCAGTTCAGACAGGGAAGGGGTCTCCCCCATGCAGGTGCCCTGGACATCAACATTg                                                                                                                           >  2:297348/1‑140 (MQ=255)              aTTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACg                                                                                                                  <  1:370362/140‑1 (MQ=255)              aTTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACg                                                                                                                  <  1:388312/140‑1 (MQ=255)               tttGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTAT‑CCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGa                                                                                                                >  1:108282/1‑140 (MQ=255)               tttGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGG‑CTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATACCGATGACCTGGACAACTACATTGCCGCCCACGGa                                                                                                                >  2:268860/1‑140 (MQ=255)                        gACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGGGACCTAGACAACAACATTGCCGCCCACGGAGTTgaaga                                                                                                        >  2:67853/1‑140 (MQ=255)                               tAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGacac                                                                                                 <  1:4141/140‑1 (MQ=255)                                     ttGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACACCATTGCCTCCCTCGTAGTTGACGAACGACACGCCttt                                                                                           >  1:308178/1‑140 (MQ=255)                                       gTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCTCACAGATTTGAAGCACGACACGCCTTTCg                                                                                         >  1:282673/1‑140 (MQ=255)                                       gTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCg                                                                                         >  2:90121/1‑140 (MQ=255)                                         ccAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAg                                                                                       <  1:579589/140‑1 (MQ=255)                                                   ttCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACACTGCCGCCCGCGGGGTTTAAGAACGAGACGCGTTTCGAGCCGATCATTg                                                                             >  2:192866/1‑140 (MQ=255)                                                    tcgtcgTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGc                                                                            >  2:523407/1‑140 (MQ=255)                                                                       tCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCt                                                         >  1:530616/1‑140 (MQ=255)                                                                           ttttACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGt                                                     <  1:523407/140‑1 (MQ=255)                                                                              tACCGGACCGATCAGTTTGGACAGGGAAGGGATATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCgg                                                  <  2:239350/140‑1 (MQ=255)                                                                               aCCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGt                                                 <  1:14788/140‑1 (MQ=255)                                                                                  ggACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCa                                              >  1:594533/1‑140 (MQ=255)                                                                                  ggACCGATCAGTTCGGACAGGGAAGGGTTAT‑CCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAg                                             <  2:108282/140‑1 (MQ=255)                                                                                    aCCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAgg                                            >  1:57638/1‑140 (MQ=255)                                                                                     ccGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCTTTGCGAGGTCGAATTGAACGCTTTTGTCGGTCCAGGt                                           >  1:224004/1‑140 (MQ=255)                                                                                        aTCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAGGTCtt                                        >  1:385377/1‑140 (MQ=255)                                                                                        aTCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAGGTCtt                                        >  1:85678/1‑140 (MQ=255)                                                                                             ttCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGAACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAGGTCTTCAttt                                   <  2:17715/140‑1 (MQ=255)                                                                                                       gAAGGGTTATCCCCCATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAGGTCTTCATTTCCGCcagcag                         >  1:439005/1‑140 (MQ=255)                                                                                                               aacccccATGCCGGTTTCCTGGACACCAACATTGCCGTCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAGGTCTTCATTTCCGCCAGCAGTTTTTTGa                 <  1:192866/138‑1 (MQ=255)                                                                                                                tCCCCCATGCCGGTGTCCTGGATAACAACTTTGCCGCGCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAGGTCTTCATTTCCGCCAGCAGTTTTTTGaa                <  1:268860/140‑1 (MQ=255)                                                                                                                     cATGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAGGTCTTCATTTCCGCCAGCAGTTTTTTGAACAGGt           >  1:145505/1‑140 (MQ=255)                                                                                                                       tGCCGGTGCCCTGGAGAACAACATTTCCGTCCACGGAATTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAGGTCTTCATTTCCGCCAGCAGTTTTTTGAACAGGTTa         <  1:297348/140‑1 (MQ=255)                                                                                                                       tGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAGGTCTTCATTTCCGCCAGCAGTTTTTTGAACAGGTTa         >  2:4788/1‑140 (MQ=255)                                                                                                                       tGCCGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAGGTCTTCATTTCCGCCAGCAGTTTTTTGAACAGGTTa         <  2:596378/140‑1 (MQ=255)                                                                                                                          cGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAGGTCTTCATTTCCGCCAGCAGTTTTTTGAACAGGTTAATg      >  2:567128/1‑140 (MQ=255)                                                                                                                          cGGTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAAGTCTTCAGTTCCGCCAGCAGTTTTTTGAACAGGTTAATg      >  2:221483/1‑140 (MQ=255)                                                                                                                            gTGACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAGGTCTTCATTTCCGCCAGCAGTTTTTTGAACAGGTTAATGtt    >  1:410405/1‑140 (MQ=255)                                                                                                                              gACCTGGACAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAGGTCTTCATTTCCGCCAGCAGTTTTTTGAACAGGTTAATGTTCa  <  2:591322/140‑1 (MQ=255)                                                                                                                              gACCTGGACAACAACAAGGCCGCCCACGGAGTTGATGAAGGACACGCCGTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAGGTCTTCATTTCCGCCAGCAGTTTTTTGAACAGGTTAATGTTCa  <  2:308178/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     ATGGTGTTAATAAATTTGTTGCTGACAATGTAAAGCTTGTCCAGACGGCCTTCGTCGTAGGCCTGCAACATCACTTTTACCGGACCGATCAGTTCGGACAGGGAAGGGTTATCCCCCATGCCGGTGACCTGGGCAACAACATTGCCGCCCACGGAGTTGAAGAACGACACGCCTTTCGAGCCGATCATTGCGAGGTCGCATTGAACGCCTTTGTCGGTCCAGGTCTTCATTTCCGCCAGCAGTTTTTTGAACAGGTTAATGTTCA  >  NC_000913/3917730‑3917994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑