Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,927,392	A→G	T80A (ACC→GCC)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,392	0	A	G	100.0%	99.1 / NA	29	T80A (ACC→GCC)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/16);  total (13/16)

TGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGG  >  NC_000913/3927271‑3927512                                                                                                                          |                                                                                                                         tgtgGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACt                                                                                                        >  1:26666/1‑140 (MQ=255)    gggggATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCa                                                                                                     >  1:399945/1‑140 (MQ=255)       ggATGGCACGCAGGACAACTTGTCGAGCTCTGAAAAAACGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAgcg                                                                                                  <  1:205038/140‑1 (MQ=255)       ggATGGCACGCAGGAAAACTTGACGTGCTTTGAAAAACCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAgcg                                                                                                  <  1:201010/140‑1 (MQ=255)        gATGGCACGCAGGATTACTTGTCGGCCTGAGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAgcgc                                                                                                 <  1:170450/140‑1 (MQ=255)        gATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAgcgc                                                                                                 <  1:148039/140‑1 (MQ=255)             cACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCg                                                                                            <  1:370628/140‑1 (MQ=255)               cGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGaa                                                                                          >  2:26819/1‑140 (MQ=255)               cGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCAAAGTGGAAACGTGAGGTCTTAGGGCGACACTACTTCAGCGCGGGCGaa                                                                                          >  2:201010/1‑140 (MQ=255)                  aGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAggg                                                                                       <  1:162153/140‑1 (MQ=255)                         cTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTaca                                                                                <  1:26819/140‑1 (MQ=255)                            gTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGc                                                                             >  2:45266/1‑140 (MQ=255)                              cGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGcac                                                                           >  1:336953/1‑140 (MQ=255)                                          aaGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCtt                                                               <  2:399945/140‑1 (MQ=255)                                               gTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGccc                                                          >  1:387452/1‑140 (MQ=255)                                                        aaaGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGa                                                 <  2:633311/140‑1 (MQ=255)                                                          aGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCCCATGAATGCCCTTAGCCCCGATGAAGAcc                                               >  1:311427/1‑140 (MQ=255)                                                               aaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCtt                                          >  1:595146/1‑140 (MQ=255)                                                               aaGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACAAGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCtt                                          >  1:325670/1‑140 (MQ=255)                                                                   tctGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTtctc                                      >  1:471752/1‑140 (MQ=255)                                                                    ctGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTcc                                     >  1:581875/1‑140 (MQ=255)                                                                      gCCTGATGCCCAGTTAGAAGTGCTTCATTTACTGGCGCAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGt                                   <  1:327960/140‑1 (MQ=255)                                                                           aTGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCAc                              <  1:587094/140‑1 (MQ=255)                                                                           aTGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCAc                              <  2:581875/140‑1 (MQ=255)                                                                                cAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGt                         <  1:45266/140‑1 (MQ=255)                                                                                  gTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCt                       <  2:336953/140‑1 (MQ=255)                                                                                                      tGGCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTgg   >  2:539406/1‑140 (MQ=255)                                                                                                       ggCGAAGTGGAAACGTCCGGCCTTAGGGCAAGACGACTTAAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTggg  <  2:256960/140‑1 (MQ=255)                                                                                                       ggCGAAGTGGAAACGTCAGGCCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTggg  <  2:26666/140‑1 (MQ=255)                                                                                                                          |                                                                                                                         TGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCACATGAAAGCCCTTCGCCCCGATGAAGACCGTCTTTCTCCGTTGCACTCGGTCTATGTTGACCAGTGGGACTGGG  >  NC_000913/3927271‑3927512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑