| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,940,176 | A→G | V146A (GTG→GCG) | hsrA ← | putative transporter HsrA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,940,176 | 0 | A | G | 97.3% | 115.9 / ‑4.4 | 37 | V146A (GTG→GCG) | hsrA | putative transporter HsrA |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (21/15); total (22/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
TGGTGAAATTGGGCATATGTTTGCGCGCGTAAAGAAGGCCCGCAATACCTATGGGGATATTGATTAAAAATATCCAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAA > NC_000913/3940042‑3940305 | tggtgAAATTGGGCATATGTTTGCGCGCGTAAAGAAGGCCCGCAATACCTATGGGGATATTGATTAAAAATATCCAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGa < 1:375613/140‑1 (MQ=255) gtgAAATTGGGCATATGTTTGCGCGCGTAAAGAAGGCCCGCAATACCTATGGGGATATTGATTAAAAATATCCAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGAcc > 1:138049/1‑140 (MQ=255) gggCATATGTTTGCGCGCGTAAAGAAGGCCCGCAATACCTATGGGGATATTGATTAAAAATATCCAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGCGGCCTAAAATTGGCCCCGCCAGACCCGGCATgg > 2:307130/1‑140 (MQ=255) tatGTTTGCGCGCGTAAAGAAGCCCCGCAATGCATATGGAGATATTGAATAAAAATATCCAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACa < 2:292395/140‑1 (MQ=255) tGCGCGCGTAAAGAAGGCCCGCAATACCTATGGGGATATTGATTAAAAATATCCAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGGCATGGCGACAAAATTc > 1:566412/1‑140 (MQ=255) tAAAGAAGGCCCGCAATACCTATGGGGATATTGATTAAAAATATCCAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTgg > 2:489633/1‑140 (MQ=255) tAAAGAAGGCCCGCAATACCTATGGGGATATTGATTAAAAATATCCAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTgg > 2:368046/1‑140 (MQ=255) agGCCCGCAATACCTATGGGGATATTGATTAAAAATATCCAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGaagaag < 2:457466/140‑1 (MQ=255) ccGCAATACCTATGGCGATATTCATCAAATATATCCATTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCa < 1:311467/140‑1 (MQ=255) ggATATTGATTAAAAATATCCAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAgcg < 2:138049/140‑1 (MQ=255) gATATTGATTAAAAATATCCAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAgcgc > 2:49847/1‑140 (MQ=255) tatTGATTAAAAATATCCAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAgcgcgc > 2:507966/1‑140 (MQ=255) tatTGATTAAAAATATCCAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAgcgcgc > 1:619318/1‑140 (MQ=255) atTGATTAAAAATATCCAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCa > 1:399796/1‑140 (MQ=255) tCCAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGa > 2:11240/1‑140 (MQ=255) tCCAGTGCCAGGTTCCCCAGGTGACCAGCACGCCGCAAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGa < 2:636963/140‑1 (MQ=255) ccAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCGGTAAGGCCAGCCGAg > 1:327744/1‑140 (MQ=255) tGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAAc < 1:562798/140‑1 (MQ=255) tGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGACATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAAc > 1:639042/1‑140 (MQ=255) cccAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGcatcatc > 1:384330/1‑140 (MQ=255) gACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGATGATAAGCGCGCAGTAATGCCAGCCGAGCAAAAGGCATCATCATTgcgc > 2:274729/1‑140 (MQ=255) gACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTgcgc > 1:13970/1‑140 (MQ=255) gACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTgcgc < 2:399796/140‑1 (MQ=255) gACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGAAGTAAGGCCAGACGCGCAACATGCATCATCATTgcgc > 1:316043/1‑140 (MQ=255) aCCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGcgcc > 1:497747/1‑140 (MQ=255) cAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGcgccgc > 2:396554/1‑140 (MQ=255) gCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCt < 1:368046/140‑1 (MQ=255) gCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCt < 2:619318/140‑1 (MQ=255) cACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTa > 2:415904/1‑140 (MQ=255) cACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTa > 1:424018/1‑140 (MQ=255) cgccgcCAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATc < 1:37442/140‑1 (MQ=255) ccgccAAGAACGGGGCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATccc < 1:49847/140‑1 (MQ=255) ggCCTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCTTTGAGCAGCCTATGCCCTGAATAACCCgg > 1:212608/1‑140 (MQ=255) ccTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGaa < 1:243935/140‑1 (MQ=255) cTAAAATTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAg < 1:489633/140‑1 (MQ=255) aaaaTTGGCCCCGCCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGTGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGAc > 1:539305/1‑140 (MQ=255) aaTTGGCCCCGCCAGTCCCGGCGTGACGAGAAAATTCCATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACaa < 1:189211/140‑1 (MQ=255) | TGGTGAAATTGGGCATATGTTTGCGCGCGTAAAGAAGGCCCGCAATACCTATGGGGATATTGATTAAAAATATCCAGTGCCAGGTTGCCCAGGTGACCAGCACGCCGCCAAGAACGGGGCCTAAAATTGGCCCCACCAGACCCGGCATGGCGACAAAATTCAATACTGGAAGAAGTTCATTACGAGGATAAGCGCGCAGTAAGGCCAGCCGAGCAACAGGCATCATCATTGCGCCGCCTATCCCCTGAATAACCCGGAAGACAA > NC_000913/3940042‑3940305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |