Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,045,212	T→C	Y338Y (TAT→TAC)	yihF →	uncharacterized protein YihF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,045,212	0	T	C	100.0%	213.2 / NA	60	Y338Y (TAT→TAC)	yihF	uncharacterized protein YihF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/34);  total (26/34)

CCTCAGCAGTGCGCCAATTTATCATCCAGTATAACATTGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTATTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAATCTTCATCATCCACAAACA  >  NC_000913/4045085‑4045342                                                                                                                                |                                                                                                                                   ccTCAGCAGTGCGCCAATTTATCATCCAGTATAACATTGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGttatta                                                                                                                        >  2:515391/1‑140 (MQ=255) ccTCAGCAGTGCGCCAATTTATCATCCAGTATAACATTGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCGCTGCAAGAAGTGAAGGTGGCATTGTTCAAAGCGTACTTACCGttattg                                                                                                                        >  2:319506/1‑139 (MQ=255)   tCAGCAGTGCGCCAATTTATCATCCAGTATAACATTGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACa                                                                                                                      >  2:35746/1‑140 (MQ=255)     agcagTGCGCCAATTTATCATCCAGTATAACATTGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACaaa                                                                                                                    >  2:466109/1‑140 (MQ=255)          tGCGCCAATTTATCATCCAGTATAACATTGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGt                                                                                                               >  2:152428/1‑140 (MQ=255)          tGCGCCAATTTATCATCCAGTATAACATTGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGt                                                                                                               >  1:605079/1‑140 (MQ=255)               cAATTTATCATCCAGTATAACATTGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGCTCAAAGAATACGTACCGTTATAACAAAAAAGTGAGcc                                                                                                          >  1:268621/1‑140 (MQ=255)                  tttATCATCCAGTATAACATTGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGAc                                                                                                       <  1:347318/140‑1 (MQ=255)                       catcCAGTATAACATTGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTa                                                                                                  <  1:97374/140‑1 (MQ=255)                           cAGTATAACATTGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAaaca                                                                                              <  2:473264/140‑1 (MQ=255)                           cAGTATAACATTGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAaaca                                                                                              <  1:644818/140‑1 (MQ=255)                              tataACATTGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTAGCGTTATTTCAAGAACGTGAGCAGACCATTAAACAAcc                                                                                           >  1:187160/1‑140 (MQ=255)                                taACATTGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCgg                                                                                         <  1:515391/140‑1 (MQ=255)                                  aCATTGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTa                                                                                       <  2:519213/140‑1 (MQ=255)                                   cATTGCGATGCAGAAGCAGCTTGCTTCACTCCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTGCAAAAAAGTGAGCCGACCATTAAACAACCGGTaa                                                                                      <  1:319506/140‑1 (MQ=255)                                      tGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGAt                                                                                   >  1:1964/1‑140 (MQ=255)                                       gCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATg                                                                                  >  1:642372/1‑140 (MQ=255)                                        cgctGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATgg                                                                                 >  2:391453/4‑140 (MQ=255)                                        cGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATgg                                                                                 >  1:374767/1‑140 (MQ=255)                                        cGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCAGATCATTAATCAACCGGTAAGATgg                                                                                 >  1:311051/1‑140 (MQ=255)                                        cGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTCAGCCGACCATTAAACAACCGGTAAGATgg                                                                                 >  1:290881/1‑140 (MQ=255)                                         gATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGga                                                                                <  2:518366/140‑1 (MQ=255)                                          aTGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACGGTTATTACTAAAAAGTAAGCCGACCATTAAGCAACCGGTAAGATGgaa                                                                               >  2:170414/1‑140 (MQ=255)                                             cAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTGATTACAAAGAAGGGAGTCGACCATTGAACATCCGGTAAGATGgaagaa                                                                            >  2:190584/1‑140 (MQ=255)                                                                acCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAAt                                                         >  1:149059/1‑140 (MQ=255)                                                                 cccTGAGTTAGCAAACGCTGAAATTGCTCTGCAAGCAGTCAATGCTGCTTTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATg                                                        <  2:188703/140‑1 (MQ=255)                                                                    tGAGTTAGCAAACGATGAAGGTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCa                                                     <  1:391453/140‑1 (MQ=255)                                                                      aGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAAt                                                   <  2:374767/140‑1 (MQ=255)                                                                      aGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAAt                                                   <  2:391727/140‑1 (MQ=255)                                                                      aGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAAt                                                   <  1:115138/140‑1 (MQ=255)                                                                         tAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTg                                                <  2:632031/140‑1 (MQ=255)                                                                         tAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTg                                                <  1:588886/140‑1 (MQ=255)                                                                         tAGCAAACGATGAAGTTGCTCTGCAAGAAGTCAATGCTGAATTGTTCAGAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTg                                                <  2:229443/140‑1 (MQ=255)                                                                         tAGCAAACGATGAAGTAGCTCTGCAAGAAGTGACTGCTGGATTGTTCACAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTg                                                <  2:290881/140‑1 (MQ=255)                                                                          aGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGGTGGAAGAGCGCACTAGGCGAAATAAATGCCAATCTgg                                               >  1:191212/1‑140 (MQ=255)                                                                          aGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTgg                                               >  2:144056/1‑140 (MQ=255)                                                                           gCAAACGATGAAGTTGCCCTGCCAGAAGTGAGTGCGGCATTGTTCAAACAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGa                                              <  2:185224/140‑1 (MQ=255)                                                                            cAAACGATGAAGTTGCTCTGCATGAAGTGAATGCTCCATCGTTCAAAGTGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGat                                             <  2:188845/140‑1 (MQ=255)                                                                            cAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAGCGCACTCGCCGAACTAAATGCCAATCTGGat                                             >  2:219353/1‑140 (MQ=255)                                                                            cAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGat                                             >  1:15468/1‑140 (MQ=255)                                                                             aaaCGATGAAGTTGCTCTGCAATAAATGAATGCTGCACTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGata                                            <  1:231995/140‑1 (MQ=255)                                                                             aaaCGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGata                                            <  2:149059/140‑1 (MQ=255)                                                                              aaCGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGatat                                           <  1:466109/140‑1 (MQ=255)                                                                                cGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGGAGATCGCCCTCGGCGAGCTAAATGCCAATCTGGATATCa                                         >  2:233133/1‑140 (MQ=255)                                                                                  aTGAAGTTGCTCTGCACTAAGTCAATGCTGCCTTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGt                                       <  2:311051/140‑1 (MQ=255)                                                                                                 aagaagTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGcc                        <  2:357632/140‑1 (MQ=255)                                                                                                  agaagTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCa                       >  1:60895/1‑140 (MQ=255)                                                                                                     agTGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAAt                    >  2:348836/1‑140 (MQ=255)                                                                                                       tGAATGCTGCATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAATCt                  <  2:1964/140‑1 (MQ=255)                                                                                                           tgctgcATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAATCTtcat              <  1:626692/140‑1 (MQ=255)                                                                                                              tgcATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAATCTTcatcat           <  1:543480/140‑1 (MQ=255)                                                                                                              tgcATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAATCTTcatcat           >  1:341394/1‑140 (MQ=255)                                                                                                               gcATTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAATCTTCatcatc          <  1:152428/140‑1 (MQ=255)                                                                                                                cATTGTTCAAAGAGTACTTACCCTTATTACATAAAAGTGCGCCGACCACTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAATCTTCATCATcc         <  2:191212/140‑1 (MQ=255)                                                                                                                 aTTGTTCAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAATCTTCATCATCca        >  2:605246/1‑140 (MQ=255)                                                                                                                   tGTTCAAAGAGTACTTAACGTTATTACAAAAAAGTGAGCTGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAATCTTCATCATCcaca      <  2:268621/140‑1 (MQ=255)                                                                                                                    gTTCAAAGAGTACTTACCGGTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAATCTTCATCATCCacaa     <  2:329759/140‑1 (MQ=255)                                                                                                                       cAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAATCTTCATCATCCacaaaca  <  2:508002/140‑1 (MQ=255)                                                                                                                       cAAAGAGTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAATCTTCATCATCCacaaaca  <  1:144056/140‑1 (MQ=255)                                                                                                                       cAAAGAGTACTTACCGTCATTACAAAAAAGTTAGCTGACGATTAAACATCCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAATCTTCATCATCCacaaaca  <  2:187160/140‑1 (MQ=255)                                                                                                                          agagTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAATCTTCATCATCcaca      >  2:27382/1‑133 (MQ=255)                                                                                                                          agagTACTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAATCTTCATCATCcaca      <  1:27382/133‑1 (MQ=255)                                                                                                                                |                                                                                                                                   CCTCAGCAGTGCGCCAATTTATCATCCAGTATAACATTGCGATGCAGAAGCAGCTTGCTGCACACCCTGAGTTAGCAAACGATGAAGTTGCTCTGCAAGAAGTGAATGCTGCATTGTTCAAAGAGTATTTACCGTTATTACAAAAAAGTGAGCCGACCATTAAACAACCGGTAAGATGGAAGAACGCACTCGGCGAACTAAATGCCAATCTGGATATCAGTATTGCCGACCCAGCCAAATCTTCATCATCCACAAACA  >  NC_000913/4045085‑4045342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑