Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,103,076	T→C	G85G (GGT→GGC)	yiiM →	2‑amino‑6‑N‑hydroxylaminopurine resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,103,076	0	T	C	100.0%	191.6 / NA	55	G85G (GGT→GGC)	yiiM	2‑amino‑6‑N‑hydroxylaminopurine resistance protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/26);  total (29/26)

GGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAG  >  NC_000913/4102942‑4103210                                                                                                                                       |                                                                                                                                       ggTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGaaaa                                                                                                                                   <  1:455045/140‑1 (MQ=255)  gTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAAc                                                                                                                                  >  2:592810/1‑140 (MQ=255)      cGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACctct                                                                                                                              <  1:118824/140‑1 (MQ=255)       gAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCtctc                                                                                                                             <  2:495682/140‑1 (MQ=255)                 agaAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGc                                                                                                                   <  1:592810/140‑1 (MQ=255)                       aGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACg                                                                                                             <  1:526117/140‑1 (MQ=255)                         gTTCACGGCGGGCCAGACAGAGCGCGGTGTCATTATCGTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGa                                                                                                           <  1:302461/140‑1 (MQ=255)                           tCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGaaa                                                                                                         >  1:428905/1‑140 (MQ=255)                            cACGGCGGGCCAGACGGAGCGCCGTCTCACTATCCTCTTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAg                                                                                                        <  1:225391/140‑1 (MQ=255)                              cggcggGCCAGACAGAGCGCTGTGTCATTATCCTAGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTa                                                                                                      <  1:488774/140‑1 (MQ=255)                                    gCCATACAGAACGCTGCGTCATTATCCCCGAGAGCAGTTTCTCTACTCGGGGCGGGAATTTCCGGAACAGGGGGGGATCTTTGTTGCGCCCGCGATTGGCGAAAACCTCTCAACCGACCGCCTCACGGCAAATAAAGttt                                                                                                >  2:155831/1‑140 (MQ=255)                                           agagCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATggg                                                                                         <  1:390805/140‑1 (MQ=255)                                              gcgcTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGa                                                                                      >  1:118977/1‑140 (MQ=255)                                                  tgtgTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGGCGGCCTGGCGGTAAATAATGTTTATATGGGCGATAtt                                                                                  >  1:213747/1‑140 (MQ=255)                                                    tgtCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATAtttt                                                                                >  2:620664/1‑140 (MQ=255)                                                       cATTATCCTCGTGAGTATTATCTCTCCTGCGCGCGGGGATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCg                                                                             <  1:179446/140‑1 (MQ=255)                                                              cTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGga                                                                      >  2:507277/1‑140 (MQ=255)                                                              cTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGga                                                                      >  1:77319/1‑140 (MQ=255)                                                              cTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGCAATGTTTATATGGGCGATATTTTCCGCTGGGga                                                                      >  1:644540/1‑140 (MQ=255)                                                                cGTGAGCATTATCTCTACTGGGTGCGGGAATTTCCCGAAGAGGCGGAGCTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGgaga                                                                    <  2:175865/140‑1 (MQ=255)                                                                cGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGgaga                                                                    >  1:422669/1‑140 (MQ=255)                                                                 gTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGagag                                                                   >  1:431799/1‑140 (MQ=255)                                                                      cATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGgaga                                                                    >  2:345884/1‑134 (MQ=255)                                                                      cATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGgaga                                                                    <  1:345884/134‑1 (MQ=255)                                                                      cATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCAtt                                                              >  1:465882/1‑140 (MQ=255)                                                                              ctACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCGTGCGTTTGGCGAAAACCTCGCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAgg                                                      <  2:392457/140‑1 (MQ=255)                                                                                aCTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATA‑TTTCCGCTGGGGAGAGGCATTAATTCAGGTcn                                                   >  2:163640/1‑139 (MQ=255)                                                                                 cTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTca                                                   >  2:424398/1‑140 (MQ=255)                                                                                   gggCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAAt                                                           <  1:114518/130‑1 (MQ=255)                                                                                   gggCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAAt                                                           >  2:114518/1‑130 (MQ=255)                                                                                   gggCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagc                                                 >  2:54434/1‑140 (MQ=255)                                                                                   gggCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagc                                                 >  2:378315/1‑140 (MQ=255)                                                                                   gggCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTGTATATGGGTGATATTTGCCGCTGGGGAGAGCCATTAATTCAGGTcagc                                                 >  2:206336/1‑140 (MQ=255)                                                                                    ggCGCTGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagcc                                                >  2:44469/1‑140 (MQ=255)                                                                                    ggCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagcc                                                >  1:635919/1‑140 (MQ=255)                                                                                     gcgcGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagcca                                               >  2:86000/1‑140 (MQ=255)                                                                                     gcgcGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagcca                                               >  1:660627/1‑140 (MQ=255)                                                                                      cgcgGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagccag                                              >  2:127211/1‑140 (MQ=255)                                                                                      cgcgGGAATTTCCGGAACAGGCGGAGGTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagccag                                              >  2:571180/1‑140 (MQ=255)                                                                                       gcgGGAATTTCCGGACCAGGCGCAGTTGTCTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagccagc                                             <  1:206336/140‑1 (MQ=255)                                                                                       gcgGGAATTTCAGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagccagc                                             >  2:558682/1‑140 (MQ=255)                                                                                         gggAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGAGAGAGGGATTAATACAGGTCAGCCAGCcg                                           >  1:246800/1‑140 (MQ=255)                                                                                         gggAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGGAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCcg                                           >  2:388424/1‑140 (MQ=255)                                                                                                 ccGGAACAGGCGGAGTTGTTTGCTGCGCCTGAGTTTGGCAAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGcc                                   <  2:246800/140‑1 (MQ=255)                                                                                                  cGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCt                                  <  1:388424/140‑1 (MQ=255)                                                                                                   ggAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCtt                                 <  2:431799/140‑1 (MQ=255)                                                                                                     aaCAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCGGGTCAGCCATCCGCGCTCGCCTTGc                               >  1:217246/1‑140 (MQ=255)                                                                                                          gCGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATaa                          <  2:635919/140‑1 (MQ=255)                                                                                                           cGGAGTTGTTTGTTGCGCCTGCGTTTGGCGAAAACCTCTCAACAGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATaaa                         <  1:531332/140‑1 (MQ=255)                                                                                                                   tttgttGCGCCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTa                 <  1:558682/140‑1 (MQ=255)                                                                                                                          cgcCTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCAtttt          >  2:601764/1‑140 (MQ=255)                                                                                                                          cgcCTGCGTTTGCCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCAtttt          >  2:457600/1‑140 (MQ=255)                                                                                                                             cTGCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGat       <  1:127211/140‑1 (MQ=255)                                                                                                                               gCGTTTGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGatat     <  2:77319/140‑1 (MQ=255)                                                                                                                                  tttGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAg  <  1:54434/140‑1 (MQ=255)                                                                                                                                  tttGGCGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAg  <  2:663324/140‑1 (MQ=255)                                                                                                                                  tttGGCGAAAACCTCTAAACCGGCGGCCTGAAGGAAAGTCATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAg  <  2:287677/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       GGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTTTATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAG  >  NC_000913/4102942‑4103210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑