| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 400,486 | A→G | R146R (CGT→CGC) | ddlA ← | D‑alanine‑‑D‑alanine ligase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 400,486 | 0 | A | G | 97.8% | 151.2 / ‑4.1 | 45 | R146R (CGT→CGC) | ddlA | D‑alanine‑‑D‑alanine ligase A |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (20/24); total (20/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.03e-01 | |||||||||||
AAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAAT > NC_000913/400350‑400620 | aagaGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGTGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGt < 2:660872/140‑1 (MQ=255)aagaGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGt > 1:666894/1‑140 (MQ=255)aagaGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGt > 1:56376/1‑140 (MQ=255) gCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGttt > 2:448630/1‑138 (MQ=255) gCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGttt < 1:448630/138‑1 (MQ=255) cccTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGGAGCAGGCGTTTggc > 2:395003/1‑139 (MQ=255) cTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGa < 2:542676/140‑1 (MQ=255) aTTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACAt > 1:665366/1‑140 (MQ=255) ttAGCCGGTTTTACAGACAGTGCTATCCCTAGTTTAGCCTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATc < 1:211652/140‑1 (MQ=255) tAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCt < 1:643027/140‑1 (MQ=255) aGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCtt < 1:131038/140‑1 (MQ=255) ccGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTg > 1:462796/1‑140 (MQ=255) ccGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTg < 1:627355/140‑1 (MQ=255) cGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGt < 1:439615/140‑1 (MQ=255) aGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGgctgc > 1:555139/1‑140 (MQ=255) aGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGgctgc > 1:283874/1‑140 (MQ=255) tGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGAAGGCGTTTGGTTACCTCTTTGACCATACAGGctgctg > 1:319000/1‑140 (MQ=255) aCCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAgg > 2:534343/1‑129 (MQ=255) aCCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAgg < 1:534343/129‑1 (MQ=255) cccAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAg < 2:582858/140‑1 (MQ=255) cccAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAg < 2:596339/140‑1 (MQ=255) cccAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAg < 1:93119/140‑1 (MQ=255) cccAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCACGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAg < 2:606670/140‑1 (MQ=255) ccAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGa < 2:508523/140‑1 (MQ=255) aGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAAc > 2:2567/1‑140 (MQ=255) tAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCa < 2:375882/140‑1 (MQ=255) gACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGa < 2:462796/140‑1 (MQ=255) aCTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGaa > 2:508551/1‑140 (MQ=255) aaaCTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTaa > 2:428599/1‑140 (MQ=255) tAATGTTGTGACGATCGGCGCGAGTCAGGGTAATAAAGGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTg < 1:268270/140‑1 (MQ=255) aTGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGc > 1:387102/1‑140 (MQ=255) tgttgtGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCg < 2:79513/140‑1 (MQ=255) tgtgACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATC‑TTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGAccc < 2:666894/140‑1 (MQ=255) tgACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGGTGCTGAAGCCAGCACATCAGAACCTCCAAATGGTAAATTGGCGACCCg > 2:268270/1‑140 (MQ=255) gcgcgcGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTcc < 2:387102/140‑1 (MQ=255) tGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGc < 2:283874/140‑1 (MQ=255) ggCGCAATGTTCAGCTCGGCATCGCATAGGAGGCGTTAGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGcc < 1:310025/140‑1 (MQ=255) ggCGCAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAATCGGTAAATCGGCAAACTGCAGCATTTCCTGTAAGGAACCTTCTTCGcc > 2:310025/1‑140 (MQ=255) cgcAATGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCa > 1:345767/1‑140 (MQ=255) aTGTTCAGCCCGGCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGt < 1:531712/140‑1 (MQ=255) ggCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCGTGCAAGGAACCATCTTCGCCCTGCGTACCGTGGACaa > 1:293758/1‑140 (MQ=255) ggCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGc > 2:459159/1‑122 (MQ=255) ggCATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGc < 1:459159/122‑1 (MQ=255) cATCGCGTAGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATc > 2:598495/1‑140 (MQ=255) aGCAGGCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAAt < 2:665366/140‑1 (MQ=255) | AAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAAT > NC_000913/400350‑400620 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |