Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,108,324	T→C	V258A (GTG→GCG)	pfkA →	6‑phosphofructokinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,108,324	0	T	C	100.0%	157.5 / NA	45	V258A (GTG→GCG)	pfkA	6‑phosphofructokinase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/29);  total (16/29)

GTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAA  >  NC_000913/4108189‑4108457                                                                                                                                        |                                                                                                                                      gTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCt                                                                                                                                   <  2:523372/140‑1 (MQ=255)   aaaaaaCACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTa                                                                                                                                 <  2:355938/140‑1 (MQ=255)   aaaaaaCACGCGATCGTGGCGAGTACCGATCATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTa                                                                                                                                 <  1:293418/140‑1 (MQ=255)        acacGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGAcc                                                                                                                            <  1:284844/140‑1 (MQ=255)          acGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACCGTGCTGGGCCACCTCCAGCGCGGTGGTTCTTCGGCGCCTTACGACCGt                                                                                                                          >  2:295826/1‑140 (MQ=255)           cgcgATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCATCGCGGTGGTTCTCCGGCGCCTTACGACCGTa                                                                                                                         >  2:296618/1‑140 (MQ=255)            gcgATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTAt                                                                                                                        >  1:441845/1‑140 (MQ=255)                   tGGCGATTACCGAACATTTGTGGGATGTTGACGAACTGGTGCATTTCAGCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCt                                                                                                                 <  2:250500/140‑1 (MQ=255)                   tGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCt                                                                                                                 <  2:616782/140‑1 (MQ=255)                     gCGATTACCGAACATCTGTGTGATGCTGATGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTc                                                                                                               <  1:212694/140‑1 (MQ=255)                                  atatGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCt                                                                                                  <  1:6813/140‑1 (MQ=255)                                  atatGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCt                                                                                                  <  1:638983/140‑1 (MQ=255)                                    atGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCTCATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTa                                                                                                <  1:612790/140‑1 (MQ=255)                                    atGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTa                                                                                                >  1:508569/1‑140 (MQ=255)                                    atGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTa                                                                                                <  1:623367/140‑1 (MQ=255)                                      gtgtgATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACg                                                                                              >  1:425544/1‑140 (MQ=255)                                      gtgtgATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACg                                                                                              >  1:392695/1‑140 (MQ=255)                                       tgtgATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGc                                                                                             <  2:167599/140‑1 (MQ=255)                                          gATGTTGACGAACTGGCGCATTTCAACGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTAt                                                                                          <  1:295826/140‑1 (MQ=255)                                           aTGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTa                                                                                                          <  2:437883/123‑1 (MQ=255)                                           aTGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTa                                                                                                          >  1:437883/1‑123 (MQ=255)                                            tGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCg                                                                                        >  1:574808/1‑140 (MQ=255)                                              ttGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGAGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGAt                                                                                      >  1:549962/1‑140 (MQ=255)                                                 aCGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTa                                                                                                >  1:523843/1‑127 (MQ=255)                                                 aCGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTa                                                                                                <  2:523843/127‑1 (MQ=255)                                                      cTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATctgctgct                                                                              >  2:130468/1‑140 (MQ=255)                                                        ggCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTgg                                                                            <  2:663829/140‑1 (MQ=255)                                                               ttCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTa                                                                     >  1:491334/1‑140 (MQ=255)                                                                      agaaagaaACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGt                                                              <  2:441845/140‑1 (MQ=255)                                                                       gaaagaaaCCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTc                                                             >  2:346489/1‑140 (MQ=255)                                                                       gaaagaaaCCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGATTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTc                                                             <  2:354486/140‑1 (MQ=255)                                                                        aaagaaaCCGGTCGTGAAACCCTCGAAACAGTGCTGGACCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGCATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCg                                                            <  1:228915/140‑1 (MQ=255)                                                                        aaagaaaCCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGCCCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCg                                                            <  1:456267/140‑1 (MQ=255)                                                                           gaaaCCGGTCGTGAAACCCGCGTAACTGTGCCGGGACACCTCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTtg                                                         <  2:195742/140‑1 (MQ=255)                                                                           gaaaCCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTtg                                                         <  2:166294/140‑1 (MQ=255)                                                                                            ccGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAgaacg                                        <  2:574808/140‑1 (MQ=255)                                                                                                gcAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAAAGAACa                                    >  1:6509/1‑140 (MQ=255)                                                                                                  aaCTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGc                                  <  2:508569/140‑1 (MQ=255)                                                                                                     tgtgCTGGGCCACATGCAGCGCGGTAGTTTTCCGGCGTCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTgg                               <  1:280908/140‑1 (MQ=255)                                                                                                         cTGGGCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGGATTCCGAAAAAACAGCTGGGTct                           >  2:346837/1‑139 (MQ=255)                                                                                                            ggCCACATCCAGCGCGGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTTGCTGTGTAGGTATACTAAACGAACAGCTGGTTcaccc                        >  2:581841/1‑139 (MQ=255)                                                                                                                        cgcgGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGa            <  2:491334/140‑1 (MQ=255)                                                                                                                          cgGTGGTTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACg          <  1:547388/140‑1 (MQ=255)                                                                                                                           ggtggtTCTCCGGCGCCCTACGACCGTATTCTGGCTTCCTGTATGGGCTCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGc         <  2:184889/140‑1 (MQ=255)                                                                                                                              ggtTCTCCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTAt      <  1:130468/140‑1 (MQ=255)                                                                                                                                  ctcCGGCGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGaa  <  1:91949/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      GTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACCGGTCGTGAAACCCGCGCAACTGTGCTGGGCCACATCCAGCGCGGTGGTTCTCCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAA  >  NC_000913/4108189‑4108457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑