Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,507	C→T	G195D (GGC→GAC)	glpF ←	glycerol facilitator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,507	0	C	T	100.0%	60.4 / NA	19	G195D (GGC→GAC)	glpF	glycerol facilitator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/12);  total (7/12)

GGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAA  >  NC_000913/4117373‑4117639                                                                                                                                       |                                                                                                                                     ggCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGTCCATa                                                                                                                                 >  2:460713/1‑140 (MQ=255)        aaGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGTCCATAg                                                                                                                                >  1:353050/1‑134 (MQ=255)        aaGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGTCCATAg                                                                                                                                <  2:353050/134‑1 (MQ=255)           cGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGTCCATAGATGCGCCaa                                                                                                                       <  2:519334/140‑1 (MQ=255)                   ggAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGTCCATAGATGCGCCAATGACCGCa                                                                                                               <  1:357256/140‑1 (MQ=255)                   ggAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGTCCATAGATGCGCCAATGACCGCa                                                                                                               >  1:626625/1‑140 (MQ=255)                                      gccgccGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGTCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCa                                                                                            >  1:150415/1‑140 (MQ=255)                                                      aCATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGTCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGcc                                                                            <  1:541780/140‑1 (MQ=255)                                                             cccAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGTCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGt                                                                     <  2:509323/140‑1 (MQ=255)                                                             cccAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGTCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGt                                                                     <  2:10253/140‑1 (MQ=255)                                                                aGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGTCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTaca                                                                  <  1:348113/140‑1 (MQ=255)                                                                 gCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGTCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTacac                                                                 >  1:88353/1‑140 (MQ=255)                                                                                  aGACTTTCGTAAAGCAGTCACGCGCTGGGTTCATGGAAAAACCTGTCAATGAGTACATAGACGCGCAAATGACCGCAATCCGTAGACCAATCAGCAAGGGAGCCAAAGGACCGCGTGGCACACCGTTCCCATCGTCCGtt                                                <  2:419288/140‑1 (MQ=255)                                                                                                 aGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGTCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGc                                 <  1:460713/140‑1 (MQ=255)                                                                                                 aGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGTCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGc                                 <  1:378209/140‑1 (MQ=255)                                                                                                           tGGGTTCATGGCAAAACCTGTCAATGGGTCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGaa                       >  1:525537/1‑140 (MQ=255)                                                                                                                   tGGCAAAACCTGTCAATGGGTCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTa               <  2:48087/140‑1 (MQ=255)                                                                                                                      cAAAACCTGTCAATGGGTCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATc            <  2:627197/140‑1 (MQ=255)                                                                                                                               tCAATGGGTCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCGAGGGTCAGCCCCATCAAAATAACGGTAATCACCATCTCa   >  2:301575/1‑140 (MQ=255)                                                                                                                                cAATGGGTGCATAGATACGCCACTGACCGCCTTGAATAGGCCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCaa  <  2:326893/140‑1 (MQ=255)                                                                                                                                cAATGGGTCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCaa  <  2:88353/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     GGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAA  >  NC_000913/4117373‑4117639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑