Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,178,657	A→G	T71A (ACC→GCC)	rplK →	50S ribosomal subunit protein L11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,178,657	0	A	G	100.0%	92.6 / NA	27	T71A (ACC→GCC)	rplK	50S ribosomal subunit protein L11
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/12);  total (15/12)

CTCTGGGTCAGCAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTACCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCC  >  NC_000913/4178526‑4178791                                                                                                                                    |                                                                                                                                       ctctGGGTCAGCAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGATTACGCTGACCTTTATTTCACGTTCGTTGCCAAGAcc                                                                                                                                >  1:242782/1‑140 (MQ=255)   ctGGGTCAGCAGGGCGTAAACATCATGAAATACTGAAAAACGTTCAACCCAAAAACTGATTCCACCGAAAAAGGTCTGCCAATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGAcccc                                                                                                                              <  2:242782/140‑1 (MQ=255)                gCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTGTTTCACTGTCGGTGGCAAGACGCCGCCGGCAGCAGtt                                                                                                                 >  1:179195/1‑140 (MQ=255)                gCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGtt                                                                                                                 <  1:514252/140‑1 (MQ=255)                gCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTCTCGTTGCCAAGACCCCGCCGGCAGCAGtt                                                                                                                 >  2:418121/1‑140 (MQ=255)                           atGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGaaaaa                                                                                                      >  1:607495/1‑140 (MQ=255)                             ggAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAg                                                                                                    >  2:435817/1‑140 (MQ=255)                                aTTCTGCAAAGCGTTCAACGCAAAAACTGATTCCACCGACAAAGGTCTCCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCgg                                                                                                 <  2:224355/140‑1 (MQ=255)                                  tCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCt                                                                                               <  2:607495/140‑1 (MQ=255)                                    tGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGCCCCCGCCTGCAACAGTTCTGCGGAAAAAAGCGGCTgg                                                                                             >  1:224355/1‑140 (MQ=255)                                    tGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTgg                                                                                             >  2:467978/1‑140 (MQ=255)                                           cGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAg                                                                                      >  1:56012/1‑140 (MQ=255)                                              tCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTATGCTGAAAAAAGCGGCTGGTATCAAGTCt                                                                                   >  1:226879/1‑140 (MQ=255)                                                aaCGCAAAAACTGATTCCATCGCAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTgg                                                                                 <  2:226879/140‑1 (MQ=255)                                                           tGATTCCATCGAAAAAGATCTGACGATTCCGCTAGTAATTACCGTTTAGGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGc                                                                      <  2:179195/140‑1 (MQ=255)                                                               tCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGaa                                                                  <  1:414004/140‑1 (MQ=255)                                                               tCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGaa                                                                  <  1:19341/140‑1 (MQ=255)                                                                         aaGGTCTGCCGATTCCGGTAGTCATCACCATTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAacaaagacaaa                                                        <  1:175407/140‑1 (MQ=255)                                                                           ggTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGt                                                      <  2:56012/140‑1 (MQ=255)                                                                                       ccGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTc                                          <  1:569821/140‑1 (MQ=255)                                                                                                    ccGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTg                             >  2:558242/1‑140 (MQ=255)                                                                                                     cGTTTACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCAGTAAGCCGAACAAAGAAAAAGTGGGTAAAAATTCCCGCGCTCAGCTGc                            >  2:261080/1‑140 (MQ=255)                                                                                                       tttACGCTGACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAg                          >  2:452645/1‑140 (MQ=255)                                                                                                               gACCGTTCTTTCACTTTCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACATAGTGGGTCAAATTTCCAGCGCTCAGCTGCAGGAAATcgc                  >  2:244676/1‑140 (MQ=255)                                                                                                                aCCGTTCTTTCACTTTCGTTGCTAAGACCCCTCCGGCAGAAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATcgcg                 <  2:283295/140‑1 (MQ=255)                                                                                                                aCCGTTCTTTCACTTTCGTTGCCAAGCCCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTAGTATCAAGTCTGGTTCCGGTAAGCCGAAAAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATcgcg                 <  2:452938/140‑1 (MQ=255)                                                                                                                            cTTTCGTTGCCAAGATCCCGCCTGCCGCAATTCTGCTAAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCt     <  1:244676/140‑1 (MQ=255)                                                                                                                               tCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGCAAAATTTCCCGCCCTCGGCTGCAGGAAATCGCGCAGACCAAAGCTGcc  >  2:263592/1‑140 (MQ=255)                                                                                                                               tCGTTGCCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGAAAAATTTCGCGCACTCTGCTGCAGGTAATCGCGCAGACCAAAGCTGcc  >  2:278419/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                       CTCTGGGTCAGCAGGGCGTAAACATCATGGAATTCTGCAAAGCGTTCAACGCAAAAACTGATTCCATCGAAAAAGGTCTGCCGATTCCGGTAGTAATCACCGTTTACGCTGACCGTTCTTTCACTTTCGTTACCAAGACCCCGCCGGCAGCAGTTCTGCTGAAAAAAGCGGCTGGTATCAAGTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCC  >  NC_000913/4178526‑4178791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑