Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,258,838	A→G	Q366R (CAG→CGG)	dinF →	DNA damage‑inducible protein F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,258,838	0	A	G	100.0%	102.7 / NA	30	Q366R (CAG→CGG)	dinF	DNA damage‑inducible protein F
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/18);  total (12/18)

GGTAGCCAGTTGCTGGATGTCTGGCGGGCAGCGTGCCGCCAGTCGGGGATCGTAGCGTTACTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCAGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGT  >  NC_000913/4258705‑4258953                                                                                                                                      |                                                                                                                    ggTAGCCAGTTGCTGGATGTCTGGCGGGCAGCGTGCCGCCAGTCGGGGATCGTAGCGTTACTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGgctggc                                                                                                               <  2:353141/140‑1 (MQ=255) ggTAGCCAGTTGCTGGATGTCTGGCGGGCAGCGTGCCGCCAGTCGGGGATCGTAGCGTTACTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGgctggc                                                                                                               <  1:556931/140‑1 (MQ=255)  gTAGCCAGTTGCTGGATGTCTGGCGGGCAGCGTGCCGCCAGTCGGGGATCGTAGCGTTACTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCggctggct                                                                                                              <  1:441598/140‑1 (MQ=255)      ccAGTTGCTGGATGTCTGGCGGGCAGCGTGCCGCCAGTCGGGGATCGTAGCGTTACTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGAcc                                                                                                          <  2:24937/140‑1 (MQ=255)      ccAGTTGCTGGATGTCTGGCGGGCAGCGTGCCGCCAGTCGGGGATCGTAGCGTTACTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGAcc                                                                                                          <  1:510851/140‑1 (MQ=255)      ccAGTTGCTGCATGTCTGGCGGGCAGCGTGCCGCCAGTCGGGGACCGTAGCGTTACTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGAcc                                                                                                          <  1:413516/140‑1 (MQ=255)        aGTTGCTGGATGTCTGGCGGGCAGCGTGCCGCCAGTCGGGGATCGTAGCGTTACTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGc                                                                                                        >  1:142025/1‑140 (MQ=255)            gCTGGATGTCTGGCGGGCAGCGTGCCGCCAGTCGGGGATCGTAGCGTTACTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAAGCCAGATGCAGCGGGTGGCTGACCGCtatc                                                                                                    >  1:186190/1‑140 (MQ=255)             cTGGATGTCTGGCGGGCAGCGTGCCGCCAGTCGGGGATCGTAGCGTTACTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGACGTTAACACAGATTGAGCGGCTGGCTGACCGCtatct                                                                                                   >  1:258709/1‑140 (MQ=255)                                       ccAGTCGGGGATCGTAGCGTTACTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCggtgg                                                                         >  1:596761/1‑140 (MQ=255)                                          gTCGGGGATCGTAGCGTTACTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGGGGCTGGCTGACCACTACCTTATCTGTCAGGAGATTTTGCCGGTGGTTg                                                                      >  2:183244/1‑140 (MQ=255)                                          gTCGGGGATCGTAGCGTTACTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTg                                                                      <  2:616473/140‑1 (MQ=255)                                              gggATCGTAGCGTTACTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGt                                                                  <  2:55988/140‑1 (MQ=255)                                               ggATCGTAGCGTTACTGCTTTCTGTGGTTTAGTTGCTGGCTGGGGAACCCATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTc                                                                 <  2:191334/140‑1 (MQ=255)                                                     tAGCGTTACTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGgtgt                                                           >  2:56342/1‑140 (MQ=255)                                                            aCTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATctgc                                                    >  2:401832/1‑140 (MQ=255)                                                            aCTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATctgc                                                    >  1:80337/1‑140 (MQ=255)                                                            aCTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATctgc                                                    >  1:89911/1‑140 (MQ=255)                                                                ttttCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGa                                                >  1:409819/1‑140 (MQ=255)                                                                 tttCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGAc                                               <  1:80900/140‑1 (MQ=255)                                                                    cGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGc                                            <  2:596761/140‑1 (MQ=255)                                                                    cGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGc                                            <  1:349627/140‑1 (MQ=255)                                                                                   tggctggGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCa                             <  2:89911/140‑1 (MQ=255)                                                                                     gctggGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAAc                           <  1:56342/140‑1 (MQ=255)                                                                                             acacATCATTGCGTTACTGACGTCGTTAACCCAGATTCATCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCa                   <  2:258709/140‑1 (MQ=255)                                                                                                catcatTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCg                <  2:409819/140‑1 (MQ=255)                                                                                                    atTGCGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGCGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAAt            >  1:353023/1‑140 (MQ=255)                                                                                                        cGTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGt        <  2:142025/140‑1 (MQ=255)                                                                                                         gTTACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTa       >  1:648625/1‑140 (MQ=255)                                                                                                          ttACTGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACTGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTaa      <  1:437456/140‑1 (MQ=255)                                                                                                              tGACGTCGTTAACCCAGATTCAGCGGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGt  <  2:648625/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                    GGTAGCCAGTTGCTGGATGTCTGGCGGGCAGCGTGCCGCCAGTCGGGGATCGTAGCGTTACTGTTTTCGGTGGTTTATTTGCTGGCTGGGGAACACATCATTGCGTTACTGACGTCGTTAACCCAGATTCAGCAGCTGGCTGACCGCTATCTTATCTGGCAGGTGATTTTGCCGGTGGTTGGCGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGT  >  NC_000913/4258705‑4258953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑