Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,292,836	A→G	Y442C (TAC→TGC)	nrfE →	putative cytochrome c‑type biogenesis protein NrfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,292,836	0	A	G	100.0%	90.0 / NA	26	Y442C (TAC→TGC)	nrfE	putative cytochrome c‑type biogenesis protein NrfE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/14);  total (12/14)

CGCGTGCAGCTTCCGGCGCTGGTAGCTCATGCGGGCGTGCTGTTATTTGCCGCTGGGGTCGTGGTCTCCAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCC  >  NC_000913/4292703‑4292970                                                                                                                                      |                                                                                                                                       cgcgTGCAGCTTCCGGCGCTGGTAGCTCATGCGGGCGTGCTGTTATTTGCCGCTGGGGTCGTGGTCTCCAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCtt                                                                                                                                  <  2:506425/140‑1 (MQ=255)      gCAGCTTCCGGCGCTGGTAGCTCATGCGGGCGTGCTGTTATTTGCCGCTGGGGTCGTGGTCTCCAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGtt                                                                                                                             >  2:378369/1‑140 (MQ=255)      gCAGCTTCCGGCGCTGGTAGCTCATGCGGGCGTGCTGTTATTTGCCGCTGGGGTCGTGGTCTCCAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGtt                                                                                                                             >  2:639441/1‑140 (MQ=255)             ccGGCGCTGGTAGCTCATGCGGGCGTGCTGTTATTTGCCGCTGGGGTCGTGGTCTCCAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTg                                                                                                                      <  1:572751/140‑1 (MQ=255)                gcgcTGGTAGCTCATGCGGGCGTGCTGTTATTTGCCGCTGGGGTCGTGGTCTCCAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCt                                                                                                                   <  2:498416/140‑1 (MQ=255)                  gcTGGTAGCTCATGCGGGCGTGCTGTTATTTGCCGCTGGGGTCGTGGTCTCCAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCg                                                                                                                 <  1:122788/140‑1 (MQ=255)                        aGCTCATGCGGGCGTGCGGTTAGTTGCCGCTGGGGCCGTCGTCTCCAGGGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTAc                                                                                                           <  2:282003/140‑1 (MQ=255)                                cGGGCGTGCTGTTATTTGCCGCTGGAGTCCTGGTCTCCAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCaaa                                                                                                   <  1:256640/140‑1 (MQ=255)                                cGGGCGTGCTGTTATGTGCCGCTGGGGTCGTGGTCTCGAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCaaa                                                                                                   <  1:228279/140‑1 (MQ=255)                                 gggCGTGCTGTTATTTGCCGCTGGGGTCGTGGTCTCCAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAg                                                                                                  <  1:368270/140‑1 (MQ=255)                                    cGTGCTGTTATTTGCCGCTGGGGTCGTGGTCTCCAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCa                                                                                               >  2:611436/1‑140 (MQ=255)                                                  ccGCTGGGGTCGTGGTCTCCAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTTCCTCGATGTACGAGACAAAGGAAATTACACCAGCGaa                                                                                 >  1:325860/1‑140 (MQ=255)                                                     cTGGGGTCGTGGTCTCCAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGaaaaa                                                                              >  1:623823/1‑140 (MQ=255)                                                           tCGTGGTCTCCAGCGCCAGCCGGCAGGAGATCAGCCTAAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATa                                                                        <  1:234571/140‑1 (MQ=255)                                                                gTCTCCAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGc                                                                   <  2:36246/140‑1 (MQ=255)                                                                    ccAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTg                                                               >  2:60867/1‑140 (MQ=255)                                                                      aGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGtt                                                             <  1:60867/140‑1 (MQ=255)                                                                           cAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGTTTGAcc                                                        >  2:125922/1‑140 (MQ=255)                                                                             gCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGTTTGACCAt                                                      <  1:599789/140‑1 (MQ=255)                                                                             gCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGTTTGACCAt                                                      >  2:456976/1‑140 (MQ=255)                                                                                gTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGTTTGACCATCAg                                                   <  1:378369/140‑1 (MQ=255)                                                                                  cAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGTTTGACCATCAGCa                                                 >  2:405486/1‑140 (MQ=255)                                                                                                  aTTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGGGGCACTGATTGGCCGTCAGCAGCGCATTGGTGAACTg                                 >  1:194929/1‑140 (MQ=255)                                                                                                             ggTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAgcg                      >  2:358347/1‑140 (MQ=255)                                                                                                              gTCAGCAGGTGACGCTGGCAGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAgcgg                     >  2:81063/1‑140 (MQ=255)                                                                                                                                 aGGATGCACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGcc  <  2:334024/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       CGCGTGCAGCTTCCGGCGCTGGTAGCTCATGCGGGCGTGCTGTTATTTGCCGCTGGGGTCGTGGTCTCCAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCC  >  NC_000913/4292703‑4292970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑