Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,299,189	G→A	P60S (CCG→TCG)	fdhF ←	formate dehydrogenase H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,299,189	0	G	A	100.0%	143.2 / NA	42	P60S (CCG→TCG)	fdhF	formate dehydrogenase H
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/28);  total (14/28)

GTCTGGATGGCATCCGGACCGTACTTCTCTTTGATGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTACCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCA  >  NC_000913/4299053‑4299324                                                                                                                                         |                                                                                                                                        gtcTGGATGGCATCCGGACCGTTCTTCTCCTTGATGGTGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGt                                                                                                                                      <  1:297253/140‑1 (MQ=255) gtcTGGATGGCATCCGGACCGTACTTCTCTTTGATGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACACTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGt                                                                                                                                      <  2:563542/140‑1 (MQ=255) gtcTGGATGGCATCCCGACCGTCCTTCTCCTTGATGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGt                                                                                                                                      <  1:191529/140‑1 (MQ=255)  tcTGGATGGCATCCGAACCGTATTTTTCTTTGATGGCCCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTc                                                                                                                                     <  1:197646/140‑1 (MQ=255)   cTGGATGGCATCCGGACCGTACTTCTCTTTGATGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCa                                                                                                                                    <  2:632051/140‑1 (MQ=255)    tGGATGGCATCCGGATCGTACTTCTTTTTTATGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAg                                                                                                                                   <  1:170795/140‑1 (MQ=255)       aTGGCATCCGGACCGTACTTCTCTTTGATGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGAt                                                                                                                                <  2:335201/140‑1 (MQ=255)        tGGCATCCGGACCGTACTTCTCTTTGATGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATc                                                                                                                               <  2:498501/140‑1 (MQ=255)           cATCCGGACCGTACTTCTCTTTGATGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGGTTGCAGGCGAGAGGTCAGGATCTgg                                                                                                                            >  1:195779/1‑140 (MQ=255)             tCCGGACCGTTCTTCTCTTTGATGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGt                                                                                                                          <  2:495356/140‑1 (MQ=255)                  aCCGTACTTCTCTTTGATGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGt                                                                                                                     <  1:56947/140‑1 (MQ=255)                       aCTTCTCTTTGATGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATg                                                                                                                <  2:634858/140‑1 (MQ=255)                            tctTTGATGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTc                                                                                                           >  2:583946/1‑140 (MQ=255)                              tttGATGGCGCTCAGGCGCTCGGCATCGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTccc                                                                                                         <  1:263161/140‑1 (MQ=255)                              tttGATGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCATGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTccc                                                                                                         >  1:167660/1‑140 (MQ=255)                                tGATGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAg                                                                                                       >  1:91591/1‑140 (MQ=255)                                 gATGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGc                                                                                                      <  1:583946/140‑1 (MQ=255)                                   tGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCa                                                                                                    >  2:451478/1‑140 (MQ=255)                                     gcgcTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCata                                                                                                  >  2:394176/1‑140 (MQ=255)                                        cTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCataata                                                                                               <  1:517520/140‑1 (MQ=255)                                          cAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAAc                                                                                             <  2:91591/140‑1 (MQ=255)                                               gcTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTc                                                                                        <  1:663448/140‑1 (MQ=255)                                                   ggCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGac                                                                                    <  1:466692/140‑1 (MQ=255)                                                    gCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCATGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGaca                                                                                   <  2:167660/140‑1 (MQ=255)                                                    gCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGaca                                                                                   <  2:361242/140‑1 (MQ=255)                                                          tAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGt                                                                             >  2:163972/1‑140 (MQ=255)                                                            aTTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTAc                                                                           <  2:655839/140‑1 (MQ=255)                                                             ttCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTAcc                                                                          <  1:86860/140‑1 (MQ=255)                                                                  tGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGCTCTGGGTATCGTTAATGAAGTCCCAGCCATAATAATCCTTCAGACACAGGGTACCCtggt                                                                     >  2:574396/1‑140 (MQ=255)                                                                  tGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTACCCtggt                                                                     >  2:19034/1‑140 (MQ=255)                                                                  tGCCTCATCCCAGGAAAAAGGTGCGAGTTTGCCGCTACGGTGGCGACGAATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTACCCtggt                                                                     <  2:204721/140‑1 (MQ=255)                                                                   gCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCAAGCCATATTAATCCTTCAGACACAGGGTACCCtggtt                                                                    >  1:250865/1‑140 (MQ=255)                                                                       cATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTACCCTggttggtt                                                                >  2:593316/1‑140 (MQ=255)                                                                       cATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTACCCTggttggtt                                                                >  1:345842/1‑140 (MQ=255)                                                                           ccAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTACCCTGGTTGGTTTTcc                                                            <  1:496752/140‑1 (MQ=255)                                                                              ggAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTACCCTGGTTGGTTTTCCCCt                                                         >  2:390720/1‑140 (MQ=255)                                                                                 aaCAGGTTCGAGTTTTCCGCCAGGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTACCCTGGTTGGTTTTCCCCTgcg                                                      <  1:264016/140‑1 (MQ=255)                                                                                                           ggCGACGGATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTACCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCg                            <  1:104190/140‑1 (MQ=255)                                                                                                                  gATCATGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTACCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATcga                     <  1:163972/140‑1 (MQ=255)                                                                                                                      aTGGGGGTTTTCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTACCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCgacgac                 <  1:451478/140‑1 (MQ=255)                                                                                                                             ttttCAGGCGCGAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTACCCTGGTTGGTTTTCCCATGCGCTGCGTCCTCCCTGACGATTTTGCCGCTATCGACGACCAGGTTg          >  2:209060/1‑140 (MQ=255)                                                                                                                                     cgcgAGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTACCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCa  <  1:593316/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GTCTGGATGGCATCCGGACCGTACTTCTCTTTGATGGCGCTCAGGCGCTCGGCAACGTAATTCAGTGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTACCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCA  >  NC_000913/4299053‑4299324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑