Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	413,449	A→G	F769L (TTC→CTC)	sbcC ←	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	413,449	0	A	G	100.0%	99.7 / NA	29	F769L (TTC→CTC)	sbcC	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/15);  total (14/15)

GCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAACGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACAT  >  NC_000913/413326‑413579                                                                                                                            |                                                                                                                                   gCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGTGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGACGCAAGGAGCGCCTGCTGATCGTCa                                                                                                                    >  1:299638/1‑140 (MQ=255) gCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCa                                                                                                                    >  2:47088/1‑140 (MQ=255)  cTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCaa                                                                                                                   <  2:111144/140‑1 (MQ=255)  cTGTGCCAGCGTTTCCGCTGTCGGATTGATCAGAGTTGGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCaa                                                                                                                   <  1:297107/140‑1 (MQ=255)       ccAGCGTTTCTGCTGCCTGAGTAACTAGATTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACg                                                                                                              <  1:174198/140‑1 (MQ=255)                    tgTCTGAGTGACCAGCGTTTGCGCCAGACAGCGCTGGCTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAgcg                                                                                                 <  1:217189/140‑1 (MQ=255)                     gTCTGAGTGACCAGAGTCTGCGCCTGACGGCCCTGCTTTTCCAGATTCCGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAgcgc                                                                                                <  2:171031/140‑1 (MQ=255)                      tCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTC‑AAGACGCTGGCCTGTAGCGCgg                                                                                              >  1:363948/1‑140 (MQ=255)                                cAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCTTTAGCGCCGCAAGGTGCGCCTGCGGATGGTCAAAGACGCTCGCCTCTAGCGCGGTGTCAAACt                                                                                     >  2:230238/1‑140 (MQ=255)                                      ttGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCt                                                                               >  2:58814/1‑140 (MQ=255)                                        gcgcCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTgg                                                                             >  2:665781/1‑140 (MQ=255)                                                        gTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACttt                                                             >  2:569227/1‑140 (MQ=255)                                                                  ttCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAg                                                   >  2:661885/1‑140 (MQ=255)                                                                   tCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAACCTGCGCCCGGGCTTTTTGCAGACTTTGCGCCGCCAGa                                                  >  1:248797/1‑140 (MQ=255)                                                                       cTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACAt                                              >  2:73466/1‑140 (MQ=255)                                                                           aGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTg                                          <  1:58814/140‑1 (MQ=255)                                                                             cTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGtt                                        >  2:385406/1‑140 (MQ=255)                                                                                     gCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAAc                                >  1:425041/1‑140 (MQ=255)                                                                                       tGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGt                              <  2:504842/140‑1 (MQ=255)                                                                                                gtttgttCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCACCGCCAGGACACCCGGTTGCTATAACGTctgctgctg                     >  1:181073/1‑140 (MQ=255)                                                                                                 tttgttCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTgctgg                    <  1:569227/140‑1 (MQ=255)                                                                                                    gttCATCCATTAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTgctggctg                 >  1:431217/1‑140 (MQ=255)                                                                                                             ttAGCGCCGCAAGGAGCCCCTGTTGATCGTCTAAGTCGCTGGCCTGTATCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCg        <  2:239428/140‑1 (MQ=255)                                                                                                              tAGCGCCGCAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACAGCCTTTTGCTGTAACGTTTGCTGCTGGCTGTGTAATGCga       >  2:200876/1‑140 (MQ=255)                                                                                                               aGCGCCGCAAGGAGCGCCTGCTGATCGTC‑AAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCgaga     <  2:363948/140‑1 (MQ=255)                                                                                                                gcgcCGCAAGGAGCGCCTGCTGTTCGTCAAAAACGATGGCCTGTAGCGTGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCgaga     <  2:248797/140‑1 (MQ=255)                                                                                                                 cgccgcAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGAc    <  2:431217/140‑1 (MQ=255)                                                                                                                  gccgcAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACa   <  1:604197/140‑1 (MQ=255)                                                                                                                  gccgcAAGGAGCGCCAGCTGATCGTCAAAGACGCTGGGCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACa   <  1:230238/140‑1 (MQ=255)                                                                                                                   ccgcAAGGAGCGCCTGTTGATCTTCAAAGACGCCGACCTGTAGCGCGGGGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACAt  <  2:299638/140‑1 (MQ=255)                                                                                                                   ccgcAAGGAGCGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCGTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTGTGCTGCTGGCTGTGTAATGCGAGACAt  <  1:73466/140‑1 (MQ=255)                                                                                                                   ccgcAAGGAGCGCCTCCTGATCCTCGAAGTCGCTGGCTTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACAt  <  1:200876/140‑1 (MQ=255)                                                                                                                            |                                                                                                                                   GCTGTGCCAGCGTTTCTGCTGTCTGAGTGACCAGAGTTTGCGCCTGACGGCGCTGGTTTTCCAGATTCTGCTTGAGCTGTTCCAGCTGCGTTAGTGTTTGTTCATCCATTAGCGCCGCAAGGAACGCCTGCTGATCGTCAAAGACGCTGGCCTGTAGCGCGGTGTCAAACTGCGCCTGGGCTTTTTGCAGACTTTGCGCCGCCAGAACATCCTGTTGCTGTAACGTCTGCTGCTGGCTGTGTAATGCGAGACAT  >  NC_000913/413326‑413579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑