Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,353,616	A→G	L368L (TTG→CTG)	lysU ←	lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,353,616	0	A	G	100.0%	151.5 / NA	44	L368L (TTG→CTG)	lysU	lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/21);  total (23/21)

GGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGAGTTTTTCAAACG  >  NC_000913/4353481‑4353750                                                                                                                                        |                                                                                                                                       ggtcggtGATTTCCGGGTTAACATCATTACGTCGTTCCAGCGGGGACATTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGcccc                                                                                                                                    <  2:218727/140‑1 (MQ=255) ggtcggtGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGcccc                                                                                                                                    >  1:577116/1‑140 (MQ=255)  gtcggtGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGTTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGccccc                                                                                                                                   <  2:3020/140‑1 (MQ=255)   tcggtGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCa                                                                                                                                  >  1:114761/1‑140 (MQ=255)    cggtGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAg                                                                                                                                 <  1:649097/140‑1 (MQ=255)      gtGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCt                                                                                                                               <  2:567166/140‑1 (MQ=255)            tCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTtctc                                                                                                                         <  1:151139/140‑1 (MQ=255)            tCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTtctc                                                                                                                         <  1:531779/140‑1 (MQ=255)                 gTTAACATCATTACGGCTTGCCCGCGGGGACCCTTTTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCg                                                                                                                    <  2:191677/140‑1 (MQ=25)                           ttACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTATGTGACAAAACGGCCCGGCCCCCAGGTTTTATCTACCGTAATACCGAt                                                                                                          >  2:178474/1‑140 (MQ=21)                               ggCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGAt                                                                                                      <  2:330781/140‑1 (MQ=255)                                   tGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAg                                                                                                  >  1:116064/1‑140 (MQ=255)                                   tGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAACTTTTCTCCACCGCAATACCGATAGATTCAg                                                                                                  >  2:297734/1‑140 (MQ=255)                                       aGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTaa                                                                                              <  1:84972/140‑1 (MQ=255)                                             gACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCttt                                                                                        >  2:388320/1‑140 (MQ=255)                                                acTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTagc                                                                                     <  2:116064/140‑1 (MQ=255)                                                         ggATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCaaa                                                                            <  2:604931/140‑1 (MQ=255)                                                          gATATTCCGTAATAACGGTTGGTTGTATCCGATGTGCCTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCaaaa                                                                           <  1:178474/140‑1 (MQ=255)                                                          gATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCCTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCaaaa                                                                           >  2:397090/1‑140 (MQ=255)                                                            tatTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAAtt                                                                         >  1:365909/1‑140 (MQ=255)                                                              ttCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTAt                                                                       >  2:535723/1‑140 (MQ=255)                                                                   taataaAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAgg                                                                  <  2:139273/140‑1 (MQ=255)                                                                    aataaAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAg                                                                   <  2:407651/138‑1 (MQ=255)                                                                    aataaAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAg                                                                   >  1:407651/1‑138 (MQ=255)                                                                        aaGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGc                                                             <  2:114761/140‑1 (MQ=255)                                                                         aGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGcc                                                            <  2:436749/140‑1 (MQ=255)                                                                                cTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATGCAGCTAATGCTTTAGCTGCATCAAAATTATACAGGTCGGCCATATCgg                                                     >  2:301329/1‑140 (MQ=255)                                                                                        gATGTGCTTCTGCCACTTCATAAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGC‑TTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGAc                                            <  1:57477/140‑1 (MQ=255)                                                                                                      aCTTCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATATTTTTTGAt                               <  1:589552/140‑1 (MQ=255)                                                                                                        ttCATCAAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATATTTTTTGATTg                             >  1:572048/1‑140 (MQ=255)                                                                                                           atcaAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATATTTTTTGATTGCtt                          <  1:388320/140‑1 (MQ=255)                                                                                                             caAAGATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATATTTTTTGATTGCTTcg                        >  2:561381/1‑140 (MQ=255)                                                                                                                 gATCTCTGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCa                    <  1:535723/140‑1 (MQ=255)                                                                                                                     tctGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTGGGCCATATCGGTGTCTGGACGATATATTTTTATTGCTTCGCGCATGGt                >  2:178263/1‑140 (MQ=255)                                                                                                                     tctGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATATTTTTTGATTGCTTcgc                       >  1:243748/1‑133 (MQ=255)                                                                                                                     tctGTGACAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATATTTTTTGATTGCTTcgc                       <  2:243748/133‑1 (MQ=255)                                                                                                                           aCAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGAGttt          >  1:369523/1‑140 (MQ=255)                                                                                                                           aCAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGAGttt          >  1:80105/1‑140 (MQ=255)                                                                                                                            cAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATCTCGGTTTCCGGATGATATTTTTTGAGTGCTGCGCGCATGGTgctgtct         >  2:251871/1‑134 (MQ=255)                                                                                                                            cAATACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGAGtttt         >  2:376489/1‑140 (MQ=255)                                                                                                                               tACGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGTTAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACAATATTTTTTGATTGATTCGCGTATGGCGAGTTTTTCa      >  1:213807/1‑140 (MQ=255)                                                                                                                                 cGTCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGAGTTTTTCaaa    >  2:342654/1‑140 (MQ=255)                                                                                                                                   tCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGAGTTTTTCAAACg  >  1:514205/1‑140 (MQ=255)                                                                                                                                   tCCCAGCCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGAGATTTTTTGATTGCTTCACGCATGGTGAGTTTTTCAAACg  >  2:246984/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       GGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATATTTTTTGATTGCTTCGCGCATGGTGAGTTTTTCAAACG  >  NC_000913/4353481‑4353750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑