Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,370,118	C→T	G99S (GGT→AGT)	yjeH ←	L‑methionine/branched chain amino acid exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,370,118	0	C	T	100.0%	129.9 / NA	40	G99S (GGT→AGT)	yjeH	L‑methionine/branched chain amino acid exporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/14);  total (26/14)

CTGGAACTGGCACCGCGAGTACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGG  >  NC_000913/4369982‑4370254                                                                                                                                         |                                                                                                                                         cTGGAACTGGCACCGCGAGTACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCAc                                                                                                                                       >  1:457229/1‑140 (MQ=255)    gAACTGGCACCGCGAGTACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACggg                                                                                                                                    >  1:575364/1‑140 (MQ=255)    gAACTGGCACCGCGAGTACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACggg                                                                                                                                    >  1:426685/1‑140 (MQ=255)         ggCACCGCGAGTACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCa                                                                                                                                        <  1:73839/131‑1 (MQ=255)         ggCACCGCGAGTACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCa                                                                                                                                        >  2:73839/1‑131 (MQ=255)         ggCACCGCGAGTACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGa                                                                                                                               >  1:501124/1‑140 (MQ=255)              cgcgAGTACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGAt                                                                                                                          >  2:534109/1‑140 (MQ=255)                  aGTACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCAAGGTATTGACCCATaaaa                                                                                                                      >  2:615271/1‑140 (MQ=255)                         atatACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAAcagcca                                                                                                               >  2:463673/1‑140 (MQ=255)                                    cagcgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTc                                                                                                    >  1:370863/1‑140 (MQ=255)                                    cagcgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTc                                                                                                    >  1:391149/1‑140 (MQ=255)                                    cagcgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTc                                                                                                    >  1:459258/1‑140 (MQ=255)                                    cagcgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTAACGGGAATGACCGAGAAAAACAGCCAGCCGGTGACTc                                                                                                    >  1:263293/1‑140 (MQ=255)                                    cagcgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTAACGGGAAGGACGGATAAAAACAGCCAGCCGGTGACTc                                                                                                    >  1:205720/1‑140 (MQ=255)                                      gcgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGTCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCATGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGc                                                                                                  >  2:437312/1‑140 (MQ=255)                                      gcgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGc                                                                                                  >  2:66929/1‑140 (MQ=255)                                      gcgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGc                                                                                                  >  1:573786/1‑140 (MQ=255)                                       cgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCt                                                                                                 >  2:648581/1‑140 (MQ=255)                                       cgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCt                                                                                                 >  1:345699/1‑140 (MQ=255)                                       cgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAAGCAAACTCACGGGAATGACCGATAATAACAACCAGCCGGTGACTCGCt                                                                                                 >  2:256627/1‑140 (MQ=255)                                            gcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGc                                                                                            >  2:568407/1‑140 (MQ=255)                                             cgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGcc                                                                                           >  1:361537/1‑140 (MQ=255)                                               tACCGAGTTCTGCCAGCAACAGATGACAGATATGCCAACCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCcgc                                                                                         <  1:256627/140‑1 (MQ=255)                                                 ccGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGCGCGGCAGGCAAACTCAGGGGAATGACCGAGAAAGACAGCCAAACGGGGACTCGCTCAAGCCGCGa                                                                                       >  2:242266/1‑140 (MQ=255)                                                    aGTTCTGCCAACAACAGTTGCCAGCTATGCCCGCCGAACTTCGCCTGGTCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAAcc                                                                                    <  2:255100/140‑1 (MQ=255)                                                            caacaaCAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCa                                                                            <  2:361537/140‑1 (MQ=255)                                                                     ttGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACcgacga                                                                   >  2:98464/1‑140 (MQ=255)                                                                     ttGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACcgacga                                                                   >  1:23707/1‑140 (MQ=255)                                                                          aGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTgc                                                              <  1:615271/140‑1 (MQ=255)                                                                                     ccaAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACgccgcc                                                   <  2:379275/140‑1 (MQ=255)                                                                                               ccTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGGGCGCGACGCCGCCTGCGCTGGGa                                         >  1:468069/1‑140 (MQ=255)                                                                                                cTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCATGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGAt                                        <  1:437312/140‑1 (MQ=255)                                                                                                     cGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTgg                                   <  2:501124/140‑1 (MQ=255)                                                                                                                              ggcaggcaAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGTCGCCGCCTTCGCCGGGATAGTGGCGTCCCAGAATCGCAAACAcaatcg          >  2:275200/1‑140 (MQ=255)                                                                                                                              ggcaggcaAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACAcaatcg          >  1:388366/1‑140 (MQ=255)                                                                                                                                  ggcaAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACAcaatcgcaat      <  2:388366/140‑1 (MQ=255)                                                                                                                                  ggcaAACTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACAcaatcgcaat      <  2:426685/140‑1 (MQ=255)                                                                                                                                      aaCTCACGGGAATGACCTATAAAAACAGCCACCCGCTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCggg  <  2:205720/140‑1 (MQ=255)                                                                                                                                      aaCTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCggg  <  2:370863/140‑1 (MQ=255)                                                                                                                                      aaCTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCggg  <  2:345699/140‑1 (MQ=255)                                                                                                                                      aaCTCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCggg  <  1:66929/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CTGGAACTGGCACCGCGAGTACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGG  >  NC_000913/4369982‑4370254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑