Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,407,624	A→G	Y324C (TAC→TGC)	rnr →	RNase R

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,407,624	0	A	G	100.0%	68.2 / NA	21	Y324C (TAC→TGC)	rnr	RNase R
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/14);  total (7/14)

GACGATGCAGTTTACTGCGAGAAAAAACGCGGCGGCGGCTGGCGTTTATGGGTCGCGATTGCCGACGTCAGCTACTATGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTACTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTGCTCTCTAACGGCCTGTGTTCGCTCAACCCGCAGGTAGACCGCCTGTGTATGGTGTGCGAGATGACGGTTTCGTCGAAAGGCCGCCTGACGGGC  >  NC_000913/4407491‑4407754                                                                                                                                      |                                                                                                                                   gACGATGCAGTTTACTGCGAGAAAAAACGCGGCGGCGGCTGGCGTTTATGGGTCGCGATTGCCGACGTCAGCTACTATGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTGCTTccc                                                                                                                              >  2:405719/1‑140 (MQ=255)           tttACTGCGAGAAAAAACGCGGCGGCGGCTGGCGTTTATGGGTCGCGATTGCCGACGTCAGCTACTATGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGtt                                                                                                                    >  2:83331/1‑140 (MQ=255)                tGCGAGAAAAAACGCGGCGGCGGCTGGCGTTTATGGGTCGCGATTGCCGACGTCAGCTACTATGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATccc                                                                                                               >  2:33565/1‑140 (MQ=255)                     gaAAAAACGCGGCGGCGGCTGGCGTTTATGGGTCGCGATTGCCGACGTCAGCTACTATGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGAtgc                                                                                                          >  2:472028/1‑140 (MQ=255)                           aCGCGGCGGCGGCTGGCGTTTATGGGTCGCGATTGCCGACGTCAGCTACTATGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCgg                                                                                                    <  2:546048/140‑1 (MQ=255)                           aCGCGGCGGCGGCTGGCGTTTATGGGTCGCGATTGCCGACGTCAGCTACTATGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCgg                                                                                                    <  1:588753/140‑1 (MQ=255)                           aCGCGGCGGCGGCTGGCATTTACGGGTCGCGATTGCCGATGTCAGCTATTATGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCgg                                                                                                    <  2:175848/140‑1 (MQ=255)                                gcggcggcTGGCGTTTTTGGGTCGCGATTGCCGACGTCAGCTACTATGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTg                                                                                               <  1:83331/140‑1 (MQ=255)                                gcggcggcTGGCGTTTATGGGTCGCGATTGCCGACGTCAGCTACTATGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTg                                                                                               <  1:12125/140‑1 (MQ=255)                                gcggcggcTGGCGTTTATGGGTCGCGATTGCCGACGTCAGCTACTATGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTg                                                                                               <  1:360640/140‑1 (MQ=255)                                gcggcggcTGGCGTTTATGGGTCGCGATTGCCGACGTCAGCTACTATGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTg                                                                                               <  1:33565/140‑1 (MQ=255)                                                          aTTGCCGACGTCAGCTACTATGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTGCTCTCTAACGGCCTGTGTTCGCTCaa                                                                     <  1:472028/140‑1 (MQ=255)                                                                       gCTACTATGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTGCTCTCTAACGGCCTGTGTTCGCTCAACCCGCAGGTAGAc                                                        <  2:1992/140‑1 (MQ=255)                                                                       gCTACTATGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTGCTCTCTAACGGCCTGTGTTCGCTCAACCCGCAGGTAGAc                                                        <  2:663074/140‑1 (MQ=255)                                                                       gCTACTATGTGCGTCCGGCAACGCCGCTGGATAGAGAAGGGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTGCTCTCTAACGGCCTGTGTTCGCTCAACCCGCAGGTAGAc                                                        <  2:286091/140‑1 (MQ=255)                                                                        ctactaTGTGGGTCCTTCAACGCCGTTGGACAGAGAACCGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTGCTCTCTAACGGCCTGTGTTCGCTCAACCCGCAGGTAGAcc                                                       <  1:326393/140‑1 (MQ=255)                                                                         tactaTGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTGCTCTCTAACGGCCTGTGTTCGCTCAACCCGCAGGTAGACCg                                                      <  1:472672/140‑1 (MQ=255)                                                                           ctaTGTGCGTCCGTCATTGCCGCTGGACAGCAAGGCGCGCAATCGTGGGACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTGCTCTCTAACGGCCTGTGTTCGCTCAACCCGCAGGTAGACCGcc                                                    <  2:324787/140‑1 (MQ=255)                                                                                                         agaAGCGCGTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTGCTCTCTAACGGCCTGTGTTCGCTCAACCCGCAGGTAGACCGCCTGTGTATGGTGTGCGAGATGACGGTTtcgt                      >  1:601023/1‑140 (MQ=255)                                                                                                              cgcgTAACCGTGGCACGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTGCTCTCTAACGGCCTGTGTTCGCTCAACCCGCAGGTAGACCTCCTGTGTGTGGCGTGCGAGATGACGGTTTCGTCGaaa                 >  1:213549/1‑140 (MQ=255)                                                                                                                             cGTCGGTGTGCTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTGCTCTCTAACGGCCTGTGTTCGCTCAACCCGCAGGTAGACCGCCTGTGTATGGTATGCGAGAGGACGGTTTCGTCGAAAGGCCGCCTGACGGGc  >  2:303302/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                   GACGATGCAGTTTACTGCGAGAAAAAACGCGGCGGCGGCTGGCGTTTATGGGTCGCGATTGCCGACGTCAGCTACTATGTGCGTCCGTCAACGCCGCTGGACAGAGAAGCGCGTAACCGTGGCACGTCGGTGTACTTCCCTTCGCAGGTTATCCCGATGCTGCCGGAAGTGCTCTCTAACGGCCTGTGTTCGCTCAACCCGCAGGTAGACCGCCTGTGTATGGTGTGCGAGATGACGGTTTCGTCGAAAGGCCGCCTGACGGGC  >  NC_000913/4407491‑4407754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑