Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,486,769	T→C	R184R (CGT→CGC)	lptF →	lipopolysaccharide transport system protein LptF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,486,769	0	T	C	100.0%	53.3 / NA	17	R184R (CGT→CGC)	lptF	lipopolysaccharide transport system protein LptF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/9);  total (8/9)

CCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGG  >  NC_000913/4486632‑4486893                                                                                                                                          |                                                                                                                             ccTGGCATGGCGGCGCTGGCGCGAGGGCAATTCCATCAATCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGccc                                                                                                                            <  2:284027/140‑1 (MQ=255)                        aGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGCCCTTCTGTGGTGGTGGCCGATTCCg                                                                                                     >  1:349995/1‑140 (MQ=255)                              aTTCCAGCAAGCGACTAGTGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGCCCTTCTGTGGTGGTGGCCGATTCCGGACAtt                                                                                               <  2:121603/140‑1 (MQ=255)                                           aCTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGCCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTgcg                                                                                  <  2:349995/140‑1 (MQ=255)                                                tGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGCCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACg                                                                             <  2:462813/140‑1 (MQ=255)                                                      cTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGCCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTccc                                                                       >  1:15505/1‑140 (MQ=255)                                                                ttCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGCCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCAc                                                             >  1:477659/1‑140 (MQ=255)                                                                 tCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGCCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCAct                                                            <  2:513846/140‑1 (MQ=255)                                                                   aTCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGCCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCTGCTGCGCGACGGCTCCCAGGTCGTCActct                                                          <  2:411881/140‑1 (MQ=255)                                                                       aaaGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGCCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGTCGACTCCCATGTCGTCACTCTCAAc                                                      >  1:282852/1‑140 (MQ=255)                                                                                   gCAGCGATGTCAAAGAAGTGTTTCTCGCGCGAATTAGACCAAAAGGTATTGCACGCCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACgcgc                                          <  2:314891/140‑1 (MQ=255)                                                                                           ttCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGCCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAgg                                  <  2:562279/140‑1 (MQ=255)                                                                                                       ttCCTCGCGCAAATTCGACCAAAAGGTAATGCACGCCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTTTCAACCAGGGATCGCGCTTCGCAGGCACTGCATTGtt                      >  1:308079/1‑140 (MQ=255)                                                                                                       ttCCTCGCGCAAATTCGACCAAAAGGTAATGCACGCCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCa                           >  1:554106/1‑135 (MQ=255)                                                                                                       ttCCTCGCGCAAATTCGACCAAAAGGTAATGCACGCCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCa                           <  2:554106/135‑1 (MQ=255)                                                                                                          cTCGCGCAAATTCGACCAAAAGGTAATGCACGCCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACg                   >  1:486804/1‑140 (MQ=255)                                                                                                               gcAAATTCGACCAAAAGGTAATGCACGCCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGAtt              >  2:394007/1‑140 (MQ=255)                                                                                                                           acagggTAATGCACGCCCTTCTCTGTTGGAGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACgg  <  1:301518/136‑1 (MQ=255)                                                                                                                                          |                                                                                                                             CCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGG  >  NC_000913/4486632‑4486893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑