Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,487,609	A→G	T98A (ACC→GCC)	lptG →	lipopolysaccharide transport system protein LptG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,487,609	0	A	G	96.2%	79.3 / NA	26	T98A (ACC→GCC)	lptG	lipopolysaccharide transport system protein LptG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (21/4);  minor base T (1/0);  total (22/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGAATGTATACCTTGCTGAGCGTGCCGAAAGATGTGCAGATCTTCTTCCCGATGGCGGCTCTGCTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTACCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGTAACTACCGTGCGCAGGCGATGTAC  >  NC_000913/4487472‑4487740                                                                                                                                           |                                                                                                                                    cAGGAATGTATACCTTGCTGAGCGTGCCGAAAGATGTGCAGATCTTCTTCCCGATGGCGGCTCTGCTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGcc                                                                                                                                   <  1:621932/140‑1 (MQ=255)               ttGCTGAGCGTGCCGAAAGATGCGCTGATTTTCTTCCCGATGGCGGCTCTGCTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGgc                                                                                                                     <  1:201533/140‑1 (MQ=255)                tGCTGAGCGTGCCGAAAGATGTGCAGATCTTCTTCCCGATGGCGGCTCTGCTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGAGATGCAGCCTTGTGA‑TTTTGCACGTATGCAGGTGgcg                                                                                                                    >  1:203968/1‑140 (MQ=255)                   tGAGCGTGCCGAAAGATGTGCAGATCTTCTTCCCGATGGCGGCTCTGCTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTg                                                                                                                 >  2:357278/1‑140 (MQ=255)                   tGAGCGTGCCGAAAGATGTGCAGATCTTCTTCCCGATGGCGGCTCTGCTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTg                                                                                                                 >  1:452488/1‑140 (MQ=255)                       cGTGCCGAAAGATGTGCAGATCTTCTTCCCGATGGCGGCTCTGCTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCgg                                                                                                             >  2:90053/1‑140 (MQ=255)                                      gCAGATCTTCTTCCCGATGGCGGCTCTGCTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCa                                                                                              <  1:500221/140‑1 (MQ=255)                                            cttcttCCCGATGGCGGCTCTGCTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TCTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGc                                                                                        >  2:242296/1‑140 (MQ=255)                                             ttcttcCCGATGGCGGCTCTGCTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGCt                                                                                       >  1:388465/1‑140 (MQ=255)                                               cttcCCGATGGCGGCTCTGCTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGGAGGGGCCCCTGTCGGTAATGAACACCGCCATTCCGCTgg                                                                                     >  2:304074/1‑140 (MQ=255)                                               cttcCCGATGGCGGCTCTGCTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGCTgg                                                                                     >  2:544939/1‑140 (MQ=255)                                                    cGATGGCGGCTCTGCTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTTCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTg                                                                                >  1:138222/1‑140 (MQ=255)                                                      aTGGCGGCTCTGCTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCt                                                                              >  1:554134/1‑140 (MQ=255)                                                       tGGCGGCTCTGCTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTg                                                                             >  1:149226/1‑140 (MQ=255)                                                                  cTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGAt                                                                  >  2:542716/1‑140 (MQ=255)                                                                          gTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCAATTCCGCCGGTGTTGCTGACGTTGGCGATTGGCGAAt                                                          >  1:171063/1‑140 (MQ=255)                                                                            tGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATAAAAACCGCCATTCCGCGGGTCTTGCAGACGATGGCGATTGGCGAATgg                                                        >  2:230668/1‑140 (MQ=255)                                                                                 ggTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTcgc                                                   >  1:541985/1‑140 (MQ=255)                                                                                   tCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCCCCATTCCGTTGGTCTTGCTGACGATTGCGACTGGCGAATGGGTCgcgc                                                 >  2:198675/1‑140 (MQ=255)                                                                                     ttGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGCTTGTCTTGCGGACGATGGTGATTGGCGAATGGGTCGcgccg                                               >  2:256682/1‑140 (MQ=255)                                                                                             cTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGa                                       >  2:103204/1‑140 (MQ=255)                                                                                             cTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGa                                       >  1:27067/1‑140 (MQ=255)                                                                                             cTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGa                                       >  1:624758/1‑140 (MQ=255)                                                                                                       gcggcgAACGGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCGGGTGGCGCTGTGGGGGATAAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGgcg                             <  2:149226/137‑1 (MQ=255)                                                                                                       gccgggAATTGGTGGTCATGAGGGCTTCTGG‑TTTTGCCGGTAGGAGGGTGCGCCGGTCGGAGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGgcg                             <  2:452488/135‑1 (MQ=255)                                                                                                       gcagcgAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGgcg                             <  1:544939/140‑1 (MQ=255)                                                                                                          gcgAACTGGTGGTGATGCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCTCGt                          >  2:485977/1‑140 (MQ=255)                                                                                                            gatcTGGTGGTGATGTGCGCTTGTGT‑TTTTGCCCTTTTGGAGGTGGGGCTGTCGGTGATAAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGTaa                        <  2:266652/137‑1 (MQ=255)                                                                                                            gAACTGGTGGGGAGGCAGG‑TTCGGGTTTTTCCCCGTATGCGGGGGGGGCTGGCGGGAAAGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGTaa                        <  2:164746/140‑1 (MQ=255)                                                                                                                          gCAGGCTTCTGG‑TTTTGCCCGTATGCAGGTGGCGCTGCCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGTAACTACCGTGCGCAgg          <  1:302889/140‑1 (MQ=255)                                                                                                                                  cTGG‑TTTTGCCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGTAACTACCGTGCGCAGGCGATGTAc  >  2:460974/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                    CAGGAATGTATACCTTGCTGAGCGTGCCGAAAGATGTGCAGATCTTCTTCCCGATGGCGGCTCTGCTTGGGGCGTTGCTTGGTCTTGGGATGCTGGCGCAGCGCAGCGAACTGGTGGTGATGCAGGCTTCTGG‑TTTTACCCGTATGCAGGTGGCGCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGTAACTACCGTGCGCAGGCGATGTAC  >  NC_000913/4487472‑4487740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑