Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,523,851	C→T	A82T (GCG→ACG)	yjhG ←	KpLE2 phage‑like element; D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,523,851	0	C	T	100.0%	122.9 / NA	37	A82T (GCG→ACG)	yjhG	KpLE2 phage‑like element; D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/16);  total (21/16)

CCCTGAGTACGCCCGTCACAAGGATCGGAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTG  >  NC_000913/4523721‑4523987                                                                                                                                   |                                                                                                                                         cccTGAGTACGCCCGTCACAAGGATCGGAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGttt                                                                                                                                 <  2:639288/140‑1 (MQ=255)    tGAGTACGCCCGTCACAAGGATCGGAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTAcc                                                                                                                              >  2:77799/1‑140 (MQ=255)    tGAGTACGCCCGTCACAAGGATCGGAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTAcc                                                                                                                              >  1:599989/1‑140 (MQ=255)           gCCCGTCACAAGGATCGGAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCGCTGGTGCCACGCCAACGTCACGGGTTTACCGTCTgcg                                                                                                                       >  1:246319/1‑140 (MQ=255)                tCACAAGGATCGGAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCcgcg                                                                                                                  >  1:347922/1‑140 (MQ=255)                tCACAAGGATCGGAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGGGCAACGCAAGCCTCGCGGGTTGACCGTCTGCGCcgcg                                                                                                                  >  1:199886/1‑140 (MQ=255)                 cacaAGGATCGGAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGt                                                                                                                 <  2:151170/140‑1 (MQ=255)                      ggATCGGAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGcc                                                                                                            <  1:623460/140‑1 (MQ=255)                      ggATCGGAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGcc                                                                                                            <  2:356693/140‑1 (MQ=255)                                 gTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTAc                                                                                                 >  2:390490/1‑140 (MQ=255)                                   acacGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTg                                                                                               <  2:589737/140‑1 (MQ=255)                                     ccGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAg                                                                                             <  1:22484/139‑1 (MQ=255)                                     acGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAg                                                                                             <  2:600642/140‑1 (MQ=255)                                     acGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAg                                                                                             <  2:562344/140‑1 (MQ=255)                                     acGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAg                                                                                             <  2:551167/140‑1 (MQ=255)                                     acGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAg                                                                                             <  1:129782/140‑1 (MQ=255)                                                   ggcaTGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCTCGTAAGCAACCCAGCGTACTGAGCAGTAAAATACCGt                                                                               >  1:291242/1‑140 (MQ=255)                                                   ggcaTGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCACGTAAGCGACCGAGGGTACTGAGCAGTAAAATACCGt                                                                               >  1:198898/1‑140 (MQ=255)                                                        ggTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGa                                                                          >  1:142615/1‑140 (MQ=255)                                                        ggTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGa                                                                          >  2:626187/1‑140 (MQ=255)                                                          ttGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATc                                                                        >  2:432192/1‑140 (MQ=255)                                                             gCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGTGTTTACCGTCTGCGCCGCGTAAGCCACCCAGTCTACTGAGCCGTAAAATACCGTCCCGATCCAg                                                                     >  2:276119/1‑140 (MQ=255)                                                               tttAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCt                                                                   >  1:577339/1‑140 (MQ=255)                                                               tttAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCt                                                                   <  1:649725/140‑1 (MQ=255)                                                                     aaCCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCg                                                             <  1:134072/140‑1 (MQ=255)                                                                     aaCCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCg                                                             <  1:432192/140‑1 (MQ=255)                                                                           ggCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGAcc                                                       >  1:515186/1‑140 (MQ=255)                                                                           ggCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGAcc                                                       >  2:420842/1‑140 (MQ=255)                                                                            gccgccGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCa                                                      >  2:39852/1‑140 (MQ=255)                                                                            gccgccGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCa                                                      >  1:89765/1‑140 (MQ=255)                                                                                                       tCGTAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATAcc                           >  1:58988/1‑140 (MQ=255)                                                                                                          tAATGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCTGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGaa                        <  2:113620/140‑1 (MQ=255)                                                                                                           aaTGCCCCTGGTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAAc                       >  1:456402/1‑140 (MQ=255)                                                                                                                cccTGGTGCAACGCCGGCGTCACGTTTTTACCGTCTGGGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATc                  <  1:276119/140‑1 (MQ=255)                                                                                                                     gTGCAACGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGc             >  2:565021/1‑140 (MQ=255)                                                                                                                         aaCGCCAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCgg         >  1:117307/1‑140 (MQ=255)                                                                                                                              cAGCGTCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGt    <  1:110127/140‑1 (MQ=255)                                                                                                                                gcgtCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTg  <  1:420842/140‑1 (MQ=255)                                                                                                                                gcgtCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCAATTCCGGCGTTACTGGGCATACCGAGCAGAGCCCCGCTCGGGCGGTTg  >  2:181655/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                         CCCTGAGTACGCCCGTCACAAGGATCGGAGACGTACACGGCATAGGGCAGGGCATGGTTGGCTTTAATAACCTCGGCCGCCGCTTTCATCTGGATGTCCAGTTCGTAATGCCCCTGGTGCAACGCCAGCGCCACGGGTTTACCGTCTGCGCCGCGTAAGCCACCGAGTGTACTGAGCAGTAAAATACCGTCCCGATCCAGCTCGTCCGGAGACCAACCCATTCCGGCATTCATGGTCATACCGAACAGATCCCCGCTCGGGCGGTTG  >  NC_000913/4523721‑4523987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑