Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,527,408	T→C	T44A (ACG→GCG)	sgcE ←	KpLE2 phage‑like element; putative epimerase SgcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,527,408	0	T	C	100.0%	139.4 / NA	39	T44A (ACG→GCG)	sgcE	KpLE2 phage‑like element; putative epimerase SgcE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/22);  total (17/22)

ATGAACGAAGATCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGC  >  NC_000913/4527274‑4527543                                                                                                                                       |                                                                                                                                        aTGAACGAAGATCCATGCGGGTCTGTTTTTAGCCAGCCCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAAtgt                                                                                                                                    <  1:174108/140‑1 (MQ=255)  tGAACGAAGATCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAAtgtt                                                                                                                                   >  2:238495/1‑140 (MQ=255)     aCGAAGATCCACGCGGGTCTGATTTCAGCCAGCGCGTCAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATgttgtt                                                                                                                                <  2:659244/140‑1 (MQ=255)               aCGCGGGTCTGATTTCAGCCAGTGCGTTAAAGCAGGGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAgctg                                                                                                                      <  2:254306/140‑1 (MQ=255)               aCGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAgctg                                                                                                                      >  1:65568/1‑140 (MQ=255)               aCGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAgctg                                                                                                                      <  1:95916/140‑1 (MQ=255)                   gggTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGt                                                                                                                  <  1:422743/140‑1 (MQ=255)                    ggTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTc                                                                                                                 <  1:579600/140‑1 (MQ=255)                      tCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCt                                                                                                               <  2:460263/140‑1 (MQ=255)                      tCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCCGCACCGTTTTCAACGCACACGCAATGTTGTGAATGAAGCTGCTGTCCt                                                                                                               >  2:311356/1‑140 (MQ=255)                         gATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCaa                                                                                                            <  1:535804/140‑1 (MQ=255)                               agccagcGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCa                                                                                                      >  1:393273/1‑140 (MQ=255)                               agccagcGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCa                                                                                                      >  1:5381/1‑140 (MQ=255)                                     cgcgTTAAACCAGCGTTGTGGCGGGTCAATCATGAAAAGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCa                                                                                                <  1:215561/140‑1 (MQ=255)                                        gTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGc                                                                                             <  1:480193/140‑1 (MQ=255)                                             aCCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTctca                                                                                        <  2:65568/140‑4 (MQ=255)                                             aCCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCa                                                                                        <  1:639631/140‑1 (MQ=255)                                                  cgcTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATc                                                                                   >  1:148761/1‑140 (MQ=255)                                                      gtgGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGa                                                                               <  2:666/140‑1 (MQ=255)                                                      gtgGCCGGGCAAGCATGAAATGAAAAGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGa                                                                               <  1:238495/140‑1 (MQ=255)                                                       tgGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGAt                                                                              >  1:651748/1‑140 (MQ=255)                                                                 aGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGc                                                                    >  2:46842/1‑140 (MQ=255)                                                                   cATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCGAGATGCAGGCTACCAAAATCCAGATTGTCCAATGCCg                                                                  >  1:281915/1‑140 (MQ=255)                                                                   cATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCg                                                                  >  1:3127/1‑140 (MQ=255)                                                                         atgaaaGGGCAAGGGATGAGGCTTCTGTCTGGCGACGGCCTGCACCGTGTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCt                                                            <  2:316991/140‑1 (MQ=255)                                                                         atgaaaGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCt                                                            <  1:46842/140‑1 (MQ=255)                                                                         atgaaaGGACAAGGGATGAGGCGTCCGTCCGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCt                                                            <  1:222271/140‑1 (MQ=255)                                                                             aaGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACg                                                        >  2:112188/1‑140 (MQ=255)                                                                                          gAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCaga                                           >  1:555596/1‑140 (MQ=255)                                                                                             gCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGa                                        >  2:122664/1‑140 (MQ=255)                                                                                               gtctgtctGGCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGAtt                                      >  1:583366/1‑140 (MQ=255)                                                                                                       ggCGACGGCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTgg                              <  1:599148/140‑1 (MQ=255)                                                                                                             ggCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTATTGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCgagag                        <  1:281582/140‑1 (MQ=255)                                                                                                              gCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCgagaga                       >  1:665984/1‑140 (MQ=255)                                                                                                              gCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCgagaga                       >  2:471648/1‑140 (MQ=255)                                                                                                              gCCTGCACCGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCgagaga                       >  2:63619/1‑140 (MQ=255)                                                                                                                      cGTTTTCATCCCAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCa               <  1:639067/140‑1 (MQ=255)                                                                                                                                ccAAACGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAg     >  1:9684/1‑140 (MQ=255)                                                                                                                                   aaCGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAgcgc  <  1:471648/140‑1 (MQ=255)                                                                                                                                   aaCGCAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAgcgc  <  2:665984/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        ATGAACGAAGATCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAATGTTGTTAATGAAGCTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGC  >  NC_000913/4527274‑4527543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑