Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,543,190	A→G	T26A (ACG→GCG)	fimA →	type 1 fimbriae major subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,543,190	0	A	G	100.0%	99.4 / NA	31	T26A (ACG→GCG)	fimA	type 1 fimbriae major subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/21);  total (10/21)

CAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCA  >  NC_000913/4543054‑4543312                                                                                                                                         |                                                                                                                           caaaGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCgcga                                                                                                                         >  2:535093/1‑140 (MQ=255)      tgtACAGACCGACTGACCATGTAGACTTACAAATAGTATTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCGGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGtt                                                                                                                    <  1:186989/140‑1 (MQ=255)      tgtACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGtt                                                                                                                    <  2:646055/140‑1 (MQ=255)        tACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGACAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTaa                                                                                                                  <  2:110149/140‑1 (MQ=255)           aGAACGACTGCCCATATCGATTTAGAAATAGTTTTTTCAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAAtgg                                                                                                               <  1:225634/140‑1 (MQ=255)                gACTGCCCATGTCGATTTAGAAATAGTTTTTTGACAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAAtgg                                                                                                               <  2:222613/135‑1 (MQ=255)                gACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAAtgg                                                                                                               >  1:222613/1‑135 (MQ=255)                  cTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACAGTTAATGGTGGGAcc                                                                                                        >  1:325323/1‑140 (MQ=255)                     cccATGTCGATTTAGAAGTAGTTTTTTGAAACGAACGCACCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGtt                                                                                                     <  2:238957/140‑1 (MQ=255)                     cccATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGtt                                                                                                     <  2:346408/140‑1 (MQ=255)                       cATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTTTACAGCGGGTCTGGCCTCTGCCGCGCCGGTGAATGGTGGGACCGTTCa                                                                                                   >  2:174005/1‑140 (MQ=255)                       cATGTCGACTTAGAAATGGTTTATTGAAAGTAATGCAGCTTGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGTTCa                                                                                                   <  2:325323/140‑1 (MQ=255)                          gTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGACCGCTGCGGCGACGGTTAATGTTGGGACCGTTCACtt                                                                                                >  1:185014/1‑140 (MQ=255)                             gATTTAGAAATAGTTTTCTGAACGGAAAGCATCATTAAAAGTAAAACTTTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTGAGTTCTACAGCGGCTCTGGCCGATGCCGCGAGGGTTAATGGTGGGACCGTTCACTTTaa                                                                                             <  2:190977/140‑1 (MQ=255)                                          ttttttGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGATCGTTCACTTGAAAGGGGAAgttgtt                                                                                >  1:277483/1‑140 (MQ=255)                                             tttGAAAGGAAAGCAGCTTGAACATTAAAACTCTGGCAATCGTTGTTCGGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAAc                                                                             <  2:277483/140‑1 (MQ=255)                                                     gaaagCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCAGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTg                                                                     <  1:454486/140‑1 (MQ=255)                                                        agcagcATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTgcgc                                                                  <  2:503970/140‑1 (MQ=255)                                                            gcaTGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGtt                                                              <  1:138535/140‑1 (MQ=255)                                                               tGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTgcg                                                                   <  1:510744/132‑1 (MQ=255)                                                               tGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTgcg                                                                   >  2:510744/1‑132 (MQ=255)                                                               tGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGAt                                                           >  2:510095/1‑140 (MQ=255)                                                                gAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATg                                                          <  1:510095/140‑1 (MQ=255)                                                                    aTTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAgg                                                      <  1:455012/140‑1 (MQ=255)                                                                      tAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGct                                                    <  1:555103/140‑1 (MQ=255)                                                                                gCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGTTCACTTTAAAGAGGAAGTTGCTAATGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCa                                          >  2:274206/1‑140 (MQ=255)                                                                                       ttgttCTGTCGGCTCCGTCCCTTAGTTCTCCAGCGGCACTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGtt                                   <  1:207299/140‑1 (MQ=255)                                                                                          ttCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTAGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAg                                >  2:510674/1‑140 (MQ=255)                                                                                               tCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCGCTATTGATCGAACCGTTCAGTTAgg                           >  1:192520/1‑140 (MQ=255)                                                                                                cGGCTCTGTCCCTCAGTTCTACAGCGGCTCTCGCCACTGCCGCGACGGATAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGa                          <  2:185014/140‑1 (MQ=255)                                                                                                cGGCTCTGTCCCTCAGTCCTACCGCGGCTCTAGCCACTGCCGCGACGGCTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGa                          <  2:190790/140‑1 (MQ=255)                                                                                                                        cGGCTCTGGCCGCTGCCGCGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGca  <  2:65329/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                           CAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGGCAATCGTTGTTCTGTCGGCTCTGTCCCTCAGTTCTACAGCGGCTCTGGCCGCTGCCACGACGGTTAATGGTGGGACCGTTCACTTTAAAGGGGAAGTTGTTAACGCCGCTTGCGCAGTTGATGCAGGCTCTGTTGATCAAACCGTTCAGTTAGGACAGGTTCGTACCGCATCGCTGGCA  >  NC_000913/4543054‑4543312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑