BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	453,249	A→G	G99G (GGT→GGC)	ampG ←	muropeptide:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	453,249	0	A	G	100.0%	115.4 / NA	34	G99G (GGT→GGC)	ampG	muropeptide:H(+) symporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/16); total (18/16)

GCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAACGTAAGCCTGGCCTAC  >  NC_000913/453112‑453383

gcgcCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGcc                                                                                                                                      >  1:1908/1‑140 (MQ=255)

 cgcCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGccc                                                                                                                                     >  2:217226/1‑140 (MQ=255)

  gcCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCa                                                                                                                                    >  2:610695/1‑140 (MQ=255)

            cGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAAt                                                                                                                          >  1:99889/1‑140 (MQ=255)

            cGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGCGTCTGGTTCGAGAAAGCCCATCGCCGCAAt                                                                                                                          >  1:47588/1‑140 (MQ=255)

              ttcttcTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATgg                                                                                                                        <  1:217226/140‑1 (MQ=255)

                  tctGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGAc                                                                                                                    <  1:30046/140‑1 (MQ=255)

                  tctGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATAACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGAc                                                                                                                    <  2:361054/140‑1 (MQ=255)

                      ccGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGTAGTTGGGTACCTTGCTATAGAAAGCCCATCTGCGCAATGGCGACtaat                                                                                                                >  2:328941/1‑140 (MQ=255)

                      ccGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTTGAGAAAGCACATCGGCGCAATGGCGACtaat                                                                                                                >  2:312304/1‑140 (MQ=255)

                       cGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACtaata                                                                                                               <  1:646019/140‑1 (MQ=255)

                                aTCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGTCTGGTTCGTGAATGCCGATCGCCGCAATGGGGACTAATAACAGGAttt                                                                                                      >  2:203941/1‑140 (MQ=255)

                                aTCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGAttt                                                                                                      >  2:563175/1‑140 (MQ=255)

                                        tCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCg                                                                                              <  1:80000/140‑1 (MQ=255)

                                                         atatCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGAAATGGCGAATAAAAACAGGATTTGCGTGGCGAGCAGCCAACCGcgccg                                                                             >  2:267835/1‑140 (MQ=255)

                                                             ccTGGGAAGCAGAGCAAAAAGCGATCACCACTGCTAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGcgccgccgc                                                                         >  2:632428/1‑140 (MQ=255)

                                                                gggAAGCAGAGCAAAAAGCGATCACTACTTCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCa                                                                      <  1:307081/140‑1 (MQ=255)

                                                                gggAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCa                                                                      <  1:163371/140‑1 (MQ=255)

                                                                 ggAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCaa                                                                     >  1:386589/1‑140 (MQ=255)

                                                                      cAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAAt                                                                >  2:329186/1‑140 (MQ=255)

                                                                         agCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGa                                                             >  2:55594/1‑140 (MQ=255)

                                                                          gCAAAAAGTGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAg                                                            <  1:66132/140‑1 (MQ=255)

                                                                                  cGATCACCTCTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAg                                                    <  1:550478/140‑1 (MQ=255)

                                                                                     tCACCACTGCCAGCGCTGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTAGCGAGCAGCCAACCGCGCCGCCGCCAAAAAAATGGAGGCGTGTAGCgg                                                 >  2:305870/1‑140 (MQ=255)

                                                                                          acTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCAt                                            <  1:414959/140‑1 (MQ=255)

                                                                                                 gcgcCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGt                                     <  2:47588/140‑1 (MQ=255)

                                                                                                 gcgcCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGt                                     <  2:105708/140‑1 (MQ=255)

                                                                                                                    ttGGGTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTaaaaa                  >  1:555824/1‑140 (MQ=255)

                                                                                                                      gggTGCCTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAACg                <  2:1908/140‑1 (MQ=255)

                                                                                                                            cTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAACGTAAGcc          >  1:592733/1‑140 (MQ=255)

                                                                                                                            cTGGTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAACGTAAGcc          >  2:124792/1‑140 (MQ=255)

                                                                                                                              ggTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAACGTAAGCCTg        <  1:55594/140‑1 (MQ=255)

                                                                                                                              ggTTCGAGAAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAACGTAAGCCTg        <  2:522990/140‑1 (MQ=255)

                                                                                                                                   gagaAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAACGTAAGCCTGGCCTa   <  2:473460/140‑1 (MQ=255)

                                                                                                                                    agaAAGCCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAACGTAAGCCTGGCCTAc  <  1:563175/140‑1 (MQ=255)

GCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTCGAGAAAACCCATCGCCGCAATGGCGACTAATAACAGGATTTGCGTGGCGAGCAGCCAACCGCGCCGCCGCCCAAAAAATGGAGGCGTGTAGCGGTCCATCAGCGGTGACCAGAGGAATTTAAAAACGTAAGCCTGGCCTAC  >  NC_000913/453112‑453383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`