| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 527,577 | T→C | intergenic (+36/‑4) | rhsD → / → ybbC | protein RhsD/PF15631 family protein YbbC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 527,577 | 0 | T | C | 98.3% | 201.2 / NA | 59 | intergenic (+36/‑4) | rhsD/ybbC | protein RhsD/PF15631 family protein YbbC |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (31/27); minor base G (1/0); total (32/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
ATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACT > NC_000913/527444‑527713 | aTGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATg > 1:666309/1‑140 (MQ=255)aTGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATg < 1:515729/140‑1 (MQ=255) cGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAata > 1:23497/1‑140 (MQ=255) gcTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCATTTATTAGGGGGGCCTATAAGGAGAGTCAATGAAATATAGTTCAatat > 2:178725/1‑140 (MQ=255) aataataaAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTc > 2:499764/1‑140 (MQ=255) taataaAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGa < 2:666309/140‑1 (MQ=255) aGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCttt > 1:119329/1‑140 (MQ=255) ggAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTc < 2:597669/140‑1 (MQ=255) gagaATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGGCTCAATGGAATCTAGTTCAATGTTTTCGA‑TGCTTTCa > 1:180704/1‑140 (MQ=255) aaTATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCAttt > 2:597164/1‑140 (MQ=255) aaTATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCAttt > 1:645704/1‑140 (MQ=255) aaTATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCAttt > 1:392867/1‑140 (MQ=255) tatTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTta < 1:441063/140‑1 (MQ=255) tatTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTta < 1:302694/140‑1 (MQ=255) ttttATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTGAATGTTCTCGA‑TGCTTTCATTTTTTATAc > 1:314355/1‑140 (MQ=255) tATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCAttttttttccact > 1:14446/1‑133 (MQ=255) tATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATAAAGTTCAATATTTTCGA‑TGCTTGCATTTTTTATACTAt > 1:251934/1‑140 (MQ=255) aTCAGAAATAGTGTCCCGGGATTGAGTTCAAGATGCATACCGTATCATTATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTAtt < 2:314355/140‑1 (MQ=255) gAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGcc < 2:576184/140‑1 (MQ=255) gAAATAGTGTCCCGGAATTGAGCTCTGTACGCATACCCTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGcc < 1:178725/140‑1 (MQ=255) aaaTAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCt > 1:122149/1‑140 (MQ=255) aaaTAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCt > 1:577075/1‑140 (MQ=255) tgtCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATg > 2:447441/1‑140 (MQ=255) ccGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGAc > 2:350534/1‑140 (MQ=255) gATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCt < 1:37626/140‑1 (MQ=255) gAGTTCAGTATGCTTCCGGTATCATGATT‑ATTTTTGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTa < 1:555189/140‑1 (MQ=255) tCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACgg < 2:577075/140‑1 (MQ=255) aTACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTa < 2:451013/128‑1 (MQ=255) aTACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTa > 1:451013/1‑128 (MQ=255) aTACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCtgatga > 1:3106/1‑140 (MQ=255) tACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGaa < 1:531661/140‑1 (MQ=255) ccGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGaaaa > 1:399654/1‑140 (MQ=255) gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACa < 2:122149/140‑1 (MQ=255) gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACa > 1:535669/1‑140 (MQ=255) gTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACa < 1:447441/140‑1 (MQ=255) aTCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACatt > 2:543073/1‑140 (MQ=255) aTCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACatt > 2:159908/1‑140 (MQ=255) aTCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACatt > 1:435763/1‑140 (MQ=255) cATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACattat > 1:454496/1‑140 (MQ=255) ttATTTTTGGTGTTTTATTTGGTGGGTCTTTAAAGGGGACTAAAT‑AAATTAAGTTTAAAAATTTCGGTTGCTTTC‑TTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTAt < 2:14446/140‑1 (MQ=255) aaTTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGAGAGCTGTTTACGGTTATGATGAGAACATTATTTTTATg > 1:326009/1‑140 (MQ=255) ttttAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAg > 2:574809/1‑140 (MQ=255) tttAGTGCTTTTATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAgg < 1:350534/140‑1 (MQ=255) tttATTAGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGaa > 1:498287/1‑140 (MQ=255) taGTGGGGCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAAtt < 2:60988/140‑1 (MQ=255) ggggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTATGGTTCTGACGAACAAATTATTTTTAGGAGGACTGTGGAAAAATTACAt > 2:303910/1‑140 (MQ=255) gggCCTATAAGGAGACTTAATGAAATATAGTTCAACATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt < 2:251934/140‑1 (MQ=255) gggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACCtt < 2:424147/140‑1 (MQ=255) gggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt < 2:435763/140‑1 (MQ=255) gggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt < 2:399654/140‑1 (MQ=255) gggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt < 1:597164/140‑1 (MQ=255) gggCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGAATGTGGAAAAATTACAtt > 1:636264/1‑140 (MQ=255) gCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt > 2:11511/1‑138 (MQ=255) gCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt > 1:93417/1‑138 (MQ=255) gCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt < 1:11511/138‑1 (MQ=255) gCCTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAtt < 2:93417/138‑1 (MQ=255) cTATAAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGa > 1:437399/1‑140 (MQ=255) tataAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGAt < 2:437399/140‑1 (MQ=255) tataAGGAGACTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGAt < 1:159908/140‑1 (MQ=255) agaCTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATAct < 2:23497/140‑1 (MQ=255) | ATGGACGATTTGAGGCGCTTAATAATAAAGGAGAATATATTTTTATCAGAAATAGTGTCCCGGGATTGAGTTCAGTATGCATACCGTATCATGATTAATTTTAGTGCTTTTATTAGTGGGGCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGA‑TGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACT > NC_000913/527444‑527713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |