Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	534,368	T→C	R151R (CGT→CGC)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	534,368	0	T	C	100.0%	55.6 / NA	17	R151R (CGT→CGC)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/13);  total (4/13)

CTGCATAAAGAAGATTTTCAGGCCGTAGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGGTTACAGTTCGTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGTTCTGGTCGTCCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTACAAACCTGCTGCCA  >  NC_000913/534240‑534480                                                                                                                                 |                                                                                                                 cTGCATAAAGAAGATTTTCAGGCCGTAGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGGTTACAGTTCGTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGCTCTGGTCgtcc                                                                                                       <  2:649972/140‑1 (MQ=255)         agaagaTTTTCAGGCGGTAGATTTTAAAGAAATTGCTCAACCGGTCAGCAAAATGGCGGTTACAGTTCGTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGCTCTGGTCgtccgggtccgg                                                                                               <  1:236003/140‑1 (MQ=255)         agaagaTTTTCAGGCCGTAGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGGTTACAGTTCGTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGCTCTGGTCgtccgggtccgg                                                                                               <  1:454145/140‑1 (MQ=255)               ttttCAGGCCGTAGATATTGAACCAATTGCTAAACCGGTGAGCAAAATGGCGGTTACAGTTCGTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGCTCTGGTCGTCCGGGTCCGGTACtgg                                                                                         <  2:249753/140‑1 (MQ=255)               ttttCAGGCCGTAGATATTGAAACAATTGCTTAACCGGTAAGCAAAATCGCGGTTACAGTTCGTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGCTCTGGTCGTCCGGGTCCGGTACtgg                                                                                         <  2:267550/140‑1 (MQ=255)                                                  cGGTCAGCCAAATGGCGGTTACGGTTCGTGAAGTGCCGCTGGTGCCTCACGTGCTGCAACAGGCATTTCACCTGATGCGCTCTGGTCGTCCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATc                                                      <  2:318100/140‑1 (MQ=255)                                                  cGGTCAGCAAAATGGTGGTTACCGTTCGTTAAGCGGCACTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGCTCTGGTCGTCCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATc                                                      <  1:185034/140‑1 (MQ=255)                                                            aaTGGCGGTTACAGTTCGTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGCTCTGGTCGTCCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATc                                            <  2:427101/140‑1 (MQ=255)                                                              tGGCGGTTACAGTTCTTGAAGCCGCCCTGGTGCCTCGGGTGCTGCAACAGGCATTTCACCTGATGCGCTCTGGTCGTCCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCt                                          <  1:207466/140‑1 (MQ=255)                                                                  ggTTACAGTTCGTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGCTCTGGTCGTCCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACa                                      <  2:356898/140‑1 (MQ=255)                                                                                    ggcgCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGCTCTGGTCGTCCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCgg                    >  1:88161/1‑140 (MQ=255)                                                                                         tGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGCTCTGGTCGTCCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTAc               <  2:88161/140‑1 (MQ=255)                                                                                           gTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGCTCTGGTCGTCCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTACaa             >  1:158565/1‑140 (MQ=255)                                                                                                tCGCGTGCTGCAACAGGCATTTCACCTGATGCGCTCTGGTCGTCCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAATTCGAGTTTCATCGTGACATGTACGAACCGCCGCCGGTCTACAAACctg        >  2:191484/1‑140 (MQ=255)                                                                                                    gTGCTGCAACAGGCATTTCACCTGATGCGCTCTGGTCGTCCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTACAAACCtgctgc    <  1:127050/140‑1 (MQ=255)                                                                                                     tgctgcAACAGGCATTTCACCTGATGCGCTCTGGTCGTCCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACGTGTACGAACCGCGGCCGGTCTACAAACCTGCTGcc   >  2:220678/1‑140 (MQ=255)                                                                                                      gctgcAACAGGCATTTCACCTGATGCGCTCTGGTCGTCCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTACAAACCTGCTGCCa  <  2:158565/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                 CTGCATAAAGAAGATTTTCAGGCCGTAGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCGGTTACAGTTCGTGAAGCGGCGCTGGTGCCTCGCGTGCTGCAACAGGCATTTCACCTGATGCGTTCTGGTCGTCCGGGTCCGGTACTGGTGGATTTACCGTTCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTACAAACCTGCTGCCA  >  NC_000913/534240‑534480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑