Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	538,610	G→A	A326A (GCG→GCA)	ybbW →	putative allantoin transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	538,610	0	G	A	100.0%	216.2 / NA	64	A326A (GCG→GCA)	ybbW	putative allantoin transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/24);  total (40/24)

ATTATTGCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCGACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGACA  >  NC_000913/538476‑538746                                                                                                                                       |                                                                                                                                         attattGCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCgg                                                                                                                                     <  2:561347/140‑1 (MQ=255) attattGCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCgg                                                                                                                                     <  2:503736/140‑1 (MQ=255)  ttattGCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGt                                                                                                                                    <  2:599357/140‑1 (MQ=255)    attGCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTGTTCTGATGACAACTGTCTCCATTAACGCAACCGGTaa                                                                                                                                  >  1:173157/1‑140 (MQ=255)    attGCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTaa                                                                                                                                  >  2:97541/1‑140 (MQ=255)      tGCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAata                                                                                                                                >  1:36336/1‑140 (MQ=255)      tGCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAata                                                                                                                                >  1:340299/1‑140 (MQ=255)        ccGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAAtatt                                                                                                                              >  1:422788/1‑140 (MQ=255)        ccGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAAtatt                                                                                                                              >  2:589450/1‑140 (MQ=255)          ggAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAAtattat                                                                                                                            >  1:368175/1‑140 (MQ=255)          ggAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAAtattat                                                                                                                            >  1:72310/1‑140 (MQ=255)          ggAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAAtattat                                                                                                                            >  2:143277/1‑140 (MQ=255)          ggAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAAtattat                                                                                                                            >  1:158366/1‑140 (MQ=255)               cAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATAACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAg                                                                                                                       <  2:422788/140‑1 (MQ=255)                       aCTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTAt                                                                                                               <  2:542208/140‑1 (MQ=255)                        cTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATc                                                                                                              >  1:357252/1‑140 (MQ=255)                            ggCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATGTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGAt                                                                                                          >  1:413756/1‑140 (MQ=255)                            ggCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGAt                                                                                                          >  2:583338/1‑140 (MQ=255)                                cTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTgcc                                                                                                      >  2:502949/1‑140 (MQ=255)                                cTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAAAGCAGCCGATAATATTAGTCCAGCCGGTTATCAGATTgcc                                                                                                      >  2:170190/1‑140 (MQ=255)                                        tGGAACGTGCTGGATATTGTTCAGCGTTGGGCCAGGCTGTTCGCCTCGATCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGc                                                                                              <  2:221867/140‑1 (MQ=255)                                           aaCGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCAcc                                                                                           >  2:356765/1‑140 (MQ=255)                                             cGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGa                                                                                         <  1:356765/140‑1 (MQ=255)                                               tGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACa                                                                                       >  1:339029/1‑140 (MQ=255)                                               tGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACa                                                                                       >  2:643691/1‑140 (MQ=255)                                               tGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACa                                                                                       >  2:396331/1‑140 (MQ=255)                                                gCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACaa                                                                                      >  2:499679/1‑140 (MQ=255)                                                 cTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGGTATCAGATTGCCGGCATTGCACCGACaaa                                                                                     >  1:241558/1‑140 (MQ=255)                                                     atatTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTg                                                                                 <  2:340299/140‑1 (MQ=255)                                                      tatTGTTCGGCGTTGGGCCATCCTGTTCGCCTCGTTCTTTGCGGTACTGGGTATTCTCATGACAACTGTCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGa                                                                                <  2:146484/140‑1 (MQ=255)                                                         tGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCt                                                                             >  1:482798/1‑140 (MQ=255)                                                                cGTTGGGACAGCCTGTTGGCCTGGTTCTTTGGGGTGCTGGTTATTCTGGTGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATaaaaa                                                                      <  2:276790/140‑1 (MQ=255)                                                                cGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGTTTGCCGCGATTGCAGCGACAACACTGACCTATaaaaa                                                                      >  1:191872/1‑140 (MQ=255)                                                                       aCAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTa                                                               <  1:436701/140‑1 (MQ=255)                                                                          gCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCTATTACACTGACAAAACAGACCCATAAAAACGGCGTACTg                                                            >  2:205128/1‑140 (MQ=255)                                                                          gCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTg                                                            >  1:488378/1‑140 (MQ=255)                                                                             tGTTCGCCTCGTTCTCTGCGGTACTCGTTTTTCTGATCACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGAtt                                                         <  1:170190/140‑1 (MQ=255)                                                                              gTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTg                                                        <  2:72310/140‑1 (MQ=255)                                                                              gTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTg                                                        <  2:36336/140‑1 (MQ=255)                                                                              gTTCGCATCGTTCTTTGCGGTACTGGTTATTCTGATGACAATTATCTCCGCCAACCGAACCGGTAATATTATTACGGCCGGTTATCACATTGCCGCCATTGCACCGACAAAACTGGCCTATAAAAACGGCGTACTGATTg                                                        <  2:413756/140‑1 (MQ=255)                                                                               ttCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGGCGCCATTGAACCAACAAAACTGACCTATAATAACGGCGTACTGATTGc                                                       >  2:210084/1‑140 (MQ=255)                                                                               ttCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGc                                                       >  1:512248/1‑140 (MQ=255)                                                                                 cGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCACCCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCa                                                     <  1:97541/140‑1 (MQ=255)                                                                                     tCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGtat                                                 >  1:65594/1‑140 (MQ=255)                                                                                          cTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCa                                            <  2:339029/140‑1 (MQ=255)                                                                                                       gTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTg                               >  2:122877/1‑140 (MQ=255)                                                                                                       gTTATTCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTg                               >  1:342827/1‑140 (MQ=255)                                                                                                           ttCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCg                           <  2:482798/140‑1 (MQ=255)                                                                                                            tCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATCAAAACGGCGTACGGATAGCCAGTATGATCAGCTTGCTGATCTGCCCGt                          >  2:178343/1‑140 (MQ=255)                                                                                                            tCTGATGACAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGt                          >  1:31056/1‑140 (MQ=255)                                                                                                                atgaCAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGaa                      >  1:407783/1‑140 (MQ=255)                                                                                                                 tgaCAACTATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGaaa                     >  2:502779/1‑140 (MQ=255)                                                                                                                        tATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATgg              <  2:31056/140‑1 (MQ=255)                                                                                                                        tATCTCCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATgg              <  1:583338/140‑1 (MQ=255)                                                                                                                          tctcCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGaa            >  1:548581/1‑140 (MQ=255)                                                                                                                          tctcCACTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGaa            >  1:480006/1‑140 (MQ=255)                                                                                                                               aCTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCa       >  2:390213/1‑140 (MQ=255)                                                                                                                               aCTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCAGCACGTGGACATTAATGGAAAATCa       >  1:309820/1‑140 (MQ=255)                                                                                                                                cTAACGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAg      <  2:158366/140‑1 (MQ=255)                                                                                                                                  aaCGCAACCGGTAATGTTATTCGAGACGGATATCAGAATGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGa    <  1:292146/140‑1 (MQ=255)                                                                                                                                  aaCGCAACCGGTAATCTTATTCCAGCCGGATATCAGACTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGa    <  1:178343/140‑1 (MQ=255)                                                                                                                                  aaCGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGa    <  1:502779/140‑1 (MQ=255)                                                                                                                                  aaCGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGa    >  2:393834/1‑140 (MQ=255)                                                                                                                                  aaCGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGa    >  1:521631/1‑140 (MQ=255)                                                                                                                                  aaCGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATCGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGa    <  1:502949/140‑1 (MQ=255)                                                                                                                                   aCGCAACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGAc   <  1:499679/140‑1 (MQ=255)                                                                                                                                    cgcaACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTTCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGACa  >  1:208469/1‑140 (MQ=255)                                                                                                                                    cgcaACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGACa  >  2:364416/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         ATTATTGCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTATCTCCACTAACGCGACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGACA  >  NC_000913/538476‑538746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑