Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	617,433	T→C	S1093P (TCG→CCG)	entF →	apo‑serine activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	617,433	0	T	C	100.0%	90.7 / NA	27	S1093P (TCG→CCG)	entF	apo‑serine activating enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/14);  total (13/14)

AGCACCCGGCGTATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGCCCGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGTCGATTATCGGCATTCAGTCACCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCT  >  NC_000913/617298‑617560                                                                                                                                        |                                                                                                                                aGCACCCGGCGTATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGCCCGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGGCTGGCAGTACAGAGTGATCTCGCGTAATCTAGATCCACAATGGCCGAt                                                                                                                             >  2:206654/1‑140 (MQ=255) aGCACCCGGCGTATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGCCCGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGAt                                                                                                                             <  1:111828/140‑1 (MQ=255)   cACCCGGCGTATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGCCCGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCAGGCAGTTCGGCGAGGTCGCGCGTTATCTCGCACCACAATGGCCGATTa                                                                                                                           >  2:313233/1‑140 (MQ=255)       cGGCGTATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGCCCGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCgg                                                                                                                       <  1:641079/140‑1 (MQ=255)        ggCGTATGGGATTCGGAACCATTCTTCCGCTGCGTGACGGTAATGGCCCGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGc                                                                                                                      <  1:273328/140‑1 (MQ=255)        ggCGTATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGCCCGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGc                                                                                                                      >  2:95988/1‑140 (MQ=255)               gggATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGCCCGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGCATTCAGt                                                                                                               <  2:135411/140‑1 (MQ=255)               gggATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGCCCGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGCATTCAGt                                                                                                               >  2:16975/1‑140 (MQ=255)                    tCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGCCCGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGCATTCAGTCACcg                                                                                                          >  2:582936/1‑140 (MQ=255)                    tCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGCCCGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCGCGATCCAGAATTGCGGATTATAGGCATTCAGTCACcg                                                                                                          >  1:197573/1‑140 (MQ=255)                      gAAACCATTCTGCCGTTGCGTGAAGGTAATGGCCCGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGCATTCAGTCACcgcg                                                                                                        <  1:442453/140‑1 (MQ=255)                                                tAATGGCCCGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGCATTCAGTCACCGCGCCCCAATGGCCCCATGCAGAcggcgg                                                                              <  2:82706/140‑1 (MQ=255)                                                  aTGGCCCGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGCATTCAGTCACCGCGCCCCAATGGCCCCATGCAGACGGCGGCa                                                                            >  2:492789/1‑140 (MQ=255)                                                      cccGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGCATTCAGTCACCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAAcc                                                                        <  1:542565/140‑1 (MQ=255)                                                          aCGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGCATTCAGTCACCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGa                                                                    >  2:589434/1‑140 (MQ=255)                                                           cGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGCATTCAGTCACCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGAt                                                                   <  2:4425/140‑1 (MQ=255)                                                                     tttCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGCATTCAGTCACCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCg                                                         >  2:151697/1‑140 (MQ=255)                                                                               gCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGCATTCAGTCACCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCt                                               >  1:154163/1‑140 (MQ=255)                                                                                cGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGCATTCAGTCACCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTg                                              <  1:492789/140‑1 (MQ=255)                                                                                                 cAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGCATTCAGTCACCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGaaca                             >  2:428489/1‑140 (MQ=255)                                                                                                    ttCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGCATTCAGTCACCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGaacaaca                          <  1:394763/140‑1 (MQ=255)                                                                                                          gTGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGCATTCAGTCACCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCa                    <  1:527988/140‑1 (MQ=255)                                                                                                           tGCTCTCGCGTTATCTCGATCCACAATGGCCGATTATCGGCATTCAGTCACCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCAt                   >  2:398453/1‑140 (MQ=255)                                                                                                            gCTCTCGCGTTATCTCGCTCCACAATGGCCGTTTAACGGCATTCAGTCCCCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATg                  <  2:197573/140‑1 (MQ=255)                                                                                                                       aTCTCGATCCACAATGGCCGATTATCGGCATTCAGTCATCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTAc       >  2:150261/1‑140 (MQ=255)                                                                                                                       aTCTCGATCCACAATGGCCGATTATCGGCATTCAGTCACCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTAc       <  1:428489/140‑1 (MQ=255)                                                                                                                       aTCTCGATCCACAATGGCCGATTATCGGCATCCAGGCACCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTAc       <  2:258104/140‑1 (MQ=255)                                                                                                                          tcGATCCACAATGGCCGATTATCGGCATTCAGTCACCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACctg    >  1:649263/1‑140 (MQ=255)                                                                                                                            gATCCACAATGGCCGATTATCGTCAGTCATTCACCGCCCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACctgct  <  1:313233/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                AGCACCCGGCGTATGGGATTCGAAACCATTCTGCCGTTGCGTGAAGGTAATGGCCCGACGCTGTTTTGTTTCCATCCTGCGTCCGGTTTTGCCTGGCAGTTCAGCGTGCTCTCGCGTTATCTCGATCCACAATGGTCGATTATCGGCATTCAGTCACCGCGCCCCAATGGCCCCATGCAGACGGCGGCAAACCTGGATGAAGTCTGCGAAGCGCATCTGGCAACGTTACTTGAACAACAACCGCATGGCCCTTATTACCTGCT  >  NC_000913/617298‑617560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑