Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	640,591	A→G	Y280C (TAC→TGC)	ahpF →	alkyl hydroperoxide reductase, AhpF component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	640,591	0	A	G	100.0%	75.1 / NA	22	Y280C (TAC→TGC)	ahpF	alkyl hydroperoxide reductase, AhpF component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/8);  total (14/8)

CCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATACGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGTACTGAAAGCACGCAGCATTATCGTGGCGACCG  >  NC_000913/640460‑640719                                                                                                                                    |                                                                                                                                 ccGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCTGGCGCACTGAATGTTCACGTTGATGAATGCGACGTTg                                                                                                                          >  1:219152/1‑140 (MQ=255) ccGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCGGGCGCACTGAAGGTGCACGTTTATGAATGCGACGTTg                                                                                                                          >  1:237266/1‑140 (MQ=255) ccGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACGGAAAGTGCACGTTAATGAATGCGACGTTg                                                                                                                          >  1:328239/1‑140 (MQ=255)              aTGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAACTTCACGTTGATCAAAGCGACGATGATGTGATCGACAg                                                                                                             >  1:285487/1‑140 (MQ=255)                     aaCGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAgc                                                                                                      <  1:417402/140‑1 (MQ=255)                      aCGTTTTGGTGGTCAGATCCTCCATACCGTTCATAACGACAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAgcg                                                                                                     <  2:237266/140‑1 (MQ=255)                                      aTCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACt                                                                                           <  2:86381/134‑1 (MQ=255)                                      aTCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACt                                                                                           >  1:86381/1‑134 (MQ=255)                                      aTCCTCGAAACCGTTGCTATCGAAAACTACTATACTGTATCGAAGACTCAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATccc                                                                                     <  2:328239/140‑1 (MQ=255)                                           cGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATGCGACGTTGATGTGATCGACAACCATAGTGCCAGCAAACTGATCCcagcag                                                                                >  1:228665/1‑140 (MQ=255)                                                 cGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTg                                                                          >  1:627485/1‑140 (MQ=255)                                                                           tACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACa                                                >  1:540342/1‑140 (MQ=255)                                                                                aaGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTc                                           >  1:58287/1‑140 (MQ=255)                                                                                            cAGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGTACt                               >  2:21450/1‑140 (MQ=255)                                                                                             aGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGTACTg                              >  2:609091/1‑140 (MQ=255)                                                                                             aGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGTACTg                              >  1:130673/1‑140 (MQ=255)                                                                                                 gCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGTACTGAAAg                          >  1:95872/1‑140 (MQ=255)                                                                                                 gCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGTACTGAAAg                          >  2:643700/1‑140 (MQ=255)                                                                                                   tGGCAGGCGCACTGAAAATTCATGTTGATGAATGCTACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGTACTGAAAGCa                        <  2:219152/140‑1 (MQ=255)                                                                                                                       cACGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGTACTGAAAGCACGCAGCATTATCGTGGCggc    <  2:130673/140‑3 (MQ=255)                                                                                                                       cACGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGTACTGAAAGCACGCAGCATTATCGTGGCggc    <  2:519922/140‑3 (MQ=255)                                                                                                                       cACGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGTACTGAAAGCACGCAGCATCATCGTGGCggc    <  1:643700/140‑3 (MQ=255)                                                                                                                         cGTTGATGAATGCGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGTACTGAAAGCACGCAGCATTATCGTGGCGGCCg  >  2:570443/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                 CCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATACGACGTTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGTACTGAAAGCACGCAGCATTATCGTGGCGACCG  >  NC_000913/640460‑640719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑