Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	645,367	A→G	M405T (ATG→ACG)	citT ←	citrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	645,367	0	A	G	100.0%	132.6 / NA	37	M405T (ATG→ACG)	citT	citrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/14);  total (23/14)

AAATAATCACCCCAGGACCGGTTGCATACGGCGTCAGACAGCCCATGATACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCATGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGGTGCGTACTCATGGTACCGGCGAAC  >  NC_000913/645233‑645492                                                                                                                                       |                                                                                                                              aaaTAATCACCCCAGGACCGGTTGCATACGGTGTCAGACGGCCCATGATACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGtt                                                                                                                          <  2:283169/140‑1 (MQ=255)      atCACCCCAGGACCGGTTGCATCCGGCGTCATACACCCCATGATACCGGTAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGgt                                                                                                                     <  2:172867/140‑1 (MQ=255)       tCACCCCAGGACCGGTTGCATACGGCGTCAGACAGCCCATGATACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCTGGAATACCGTTAGCGAAGGCCAGAAGAACCAGCAGCGTGGTTGCGgtg                                                                                                                    >  2:181972/1‑140 (MQ=255)       tCACCCCAGGACCGGTTGCATACGGCGTCAGACAGCCCATGATACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGgtg                                                                                                                    >  1:347122/1‑140 (MQ=255)       tCACCCCAGGACCGGTTGCATACGGCGTCAGACAGCCCATGATACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGgtg                                                                                                                    >  2:421760/1‑140 (MQ=255)             cAGGACCGGTTGCATACGGCGTCAGACAGCCCATGATACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCa                                                                                                              >  2:457702/1‑140 (MQ=255)             cAGGACCGGTTGCATACGGCGTCAGACAGCCCATGATACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCa                                                                                                              >  2:509285/1‑140 (MQ=255)                   cGGTTGCATACGGCGTCAGACAGCCCATGATACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAgg                                                                                                        <  2:459280/140‑1 (MQ=255)                     gTTGCATACGGCGTCAGACAGCCCATGATACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCt                                                                                                      >  1:486756/1‑140 (MQ=255)                     gTTGCATACGGCGTCAGACAGCCCATGATACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCt                                                                                                      >  2:546504/1‑140 (MQ=255)                     gTTGCATACGGCGTCAGACAGCCCATGATACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCt                                                                                                      >  1:454129/1‑140 (MQ=255)                     gTTGCATACGGCGTCAGACAGCCCATGATACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCATGTTTGCGGCGTGCGCAGACAGGCt                                                                                                      >  1:281653/1‑140 (MQ=255)                                 gTCAGACAGCCCATGATACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTa                                                                                          <  2:380852/140‑1 (MQ=255)                                      aCAGCCCGTGATACCGATAGACAGCACCAGCAGGATACAGAGTTGTTCCATTGGTACGCCGGGAATACCTTTACCGTCGGCCAGAATAACCGGCAGCGTGGTTGCGGGGTGCGCAGACAGGCTGGCAAACAGGGAGtgtg                                                                                     <  2:497735/140‑1 (MQ=255)                                           ccATGATACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAg                                                                                <  1:509285/140‑1 (MQ=255)                                               gATACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGa                                                                            <  2:454129/140‑1 (MQ=255)                                                aTACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGaa                                                                           <  1:457702/140‑1 (MQ=255)                                                    cGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCGGACAGGCTGGCAAACAAGTAGGGTGCAAAGTAGAACAcc                                                                       >  2:202459/1‑140 (MQ=255)                                                      aTAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAg                                                                     <  2:600249/140‑1 (MQ=255)                                                          aCAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAAcc                                                                 >  1:30224/1‑140 (MQ=255)                                                              cACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTa                                                             >  1:122658/1‑140 (MQ=255)                                                              cACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTa                                                             >  1:28747/1‑140 (MQ=255)                                                               aCCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTAc                                                            >  1:553834/1‑140 (MQ=255)                                                                  agcagGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTACAAt                                                         <  1:527032/140‑1 (MQ=255)                                                                         tACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCCAACAGGTAGTGTGCAAAGTAGAACAACAGAGCCAGTACAATCACCGtt                                                  >  2:188167/1‑140 (MQ=255)                                                                                 tgttCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTgg                                          >  2:531358/1‑140 (MQ=255)                                                                                   ttCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAAAACCAGAACAATCACCGTTGCGTTTGGTg                                        >  1:288042/1‑140 (MQ=255)                                                                                     ccATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTgag                                      >  2:407321/1‑140 (MQ=255)                                                                                             aCGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTc                              <  2:486756/140‑1 (MQ=255)                                                                                                     aaTACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGGTGCg                      <  1:133788/140‑1 (MQ=255)                                                                                                     aaTACCTTTACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGGTGCg                      >  1:378364/1‑140 (MQ=255)                                                                                                           tttACCGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGGTGCGTACTCa                >  2:439584/1‑140 (MQ=255)                                                                                                               ccGACGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTATAATCACCGATGCGTTTGGTGAGAATGCTTCTAGGTGCGTACTCATGGt            >  2:228681/1‑140 (MQ=255)                                                                                                                   cGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGGTGCGTa                    >  1:81322/1‑128 (MQ=255)                                                                                                                   cGGCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGGTGCGTa                    <  2:81322/128‑1 (MQ=255)                                                                                                                    ggCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGGTGCGTACTCATGGTACCgg       >  1:514246/1‑140 (MQ=255)                                                                                                                    ggCCAGAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGGTTGGTGAGAATCCTTCCAGGCGTGTACTCATGGTACCgg       >  2:537174/1‑140 (MQ=255)                                                                                                                         gAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGGTGCGTACTCATGGTACCGGCGAAc  <  2:30224/140‑1 (MQ=255)                                                                                                                         gAATAACCGGCAGCGTGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGGTGCGTACTCATGGTACCGGCGAAc  <  2:122658/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                              AAATAATCACCCCAGGACCGGTTGCATACGGCGTCAGACAGCCCATGATACCGATAGACAGCACCAGCAGGATACACAGTTGTTCCATTGGTACGCCCGGAATACCTTTACCGACGGCCAGAATAACCGGCAGCATGGTTGCGGTGTGCGCAGACAGGCTGGCAAACAGGTAGTGTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGGTGCGTACTCATGGTACCGGCGAAC  >  NC_000913/645233‑645492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑