Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	657,830	T→C	T37A (ACG→GCG)	crcB ←	F(‑) channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	657,830	0	T	C	100.0%	112.7 / NA	32	T37A (ACG→GCG)	crcB	F(‑) channel
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/9);  total (23/9)

GCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTAT  >  NC_000913/657714‑657965                                                                                                                     |                                                                                                                                        gCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCa                                                                                                                  <  1:11723/140‑1 (MQ=255)  ccACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGaa                                                                                                                              <  1:106790/127‑1 (MQ=255)  ccACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGaa                                                                                                                              >  2:106790/1‑127 (MQ=255)      aaaaTCCGGTGGTGATTAATACATTCCACACTGGATCTATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTggg                                                                                                             <  1:293570/140‑1 (MQ=255)                ggtgATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCa                                                                                                   >  2:9104/1‑140 (MQ=255)                 gtgATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCAt                                                                                                  <  2:43265/140‑1 (MQ=255)                         tACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACa                                                                                          <  1:413038/140‑1 (MQ=255)                                   acTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGc                                                                                >  1:481331/1‑140 (MQ=255)                                   acTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGc                                                                                >  1:88996/1‑140 (MQ=255)                                      ggATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCAc                                                                             >  1:131589/1‑140 (MQ=255)                                      ggATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCAc                                                                             <  2:517305/140‑1 (MQ=255)                                      ggATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCAc                                                                             >  1:458752/1‑140 (MQ=255)                                       gATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACg                                                                            >  2:567963/1‑140 (MQ=255)                                       gATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACg                                                                            <  2:5354/140‑1 (MQ=255)                                       gATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACg                                                                            <  1:9104/140‑1 (MQ=255)                                             tGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCTTACTCACCAGCCATCTCGCCACGCTTccc                                                                      >  1:219476/1‑140 (MQ=255)                                               ttCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCg                                                                                 >  2:468318/1‑127 (MQ=255)                                               ttCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCg                                                                                 <  1:468318/127‑1 (MQ=255)                                                 cGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAAAAGACATCTCGCCACGCTTCCCGTac                                                                  >  1:238934/1‑140 (MQ=255)                                                       ccTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGc                                                            >  1:647227/1‑140 (MQ=255)                                                              aaCCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAGCAGGCATGCCGCCACGTTTCCCGTACCACCTCCAATaaa                                                     >  2:323052/1‑140 (MQ=255)                                                                ccATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATaaaac                                                   >  1:161682/1‑139 (MQ=255)                                                                   tGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATAATTAACAGCGATCTCGCTACGCTTCCCGTACCACCGCCAATAAAAACTg                                                >  2:281603/1‑140 (MQ=255)                                                                         tccttttcttatGATGAATCCCCCAATAGGTTTTGCTGTCAGCCCCCCCAACGGAACCCCCTGATGCGGTGGTTAAAATCGCATACTAAACACCCATCCCGCCCCGCTCCCCGTACCACCGCCAATAAAAACTGCTaaaa                                          <  2:131589/140‑1 (MQ=255)                                                                          cctattcctatGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAaag                                         >  1:412519/1‑140 (MQ=255)                                                                           cttttcatatGATGAAGGCCCCAAACAGTTTTGGTCTCACCCCCCCCAACGAAAACGCTTGATGCGGTGGTTTAAACCGCATACTAACCAGCCATCCCCCCCCGCTCCCCGTCCCACCGCCAATAAAAACTGCTAAaaga                                        <  2:458752/137‑1 (MQ=255)                                                                           ctattcttatGATAAACGCCCAAATAAGGTTTGCTTCCAGCGCCCCCACCGAAATCCCCTGATGCAGGGGGTTAAATCCCATACTTAACAGCAATCTCGCCACCCTTCCCGTACCACCGCCAATAAAAACTGCTAAaaga                                        <  2:20369/140‑1 (MQ=255)                                                                                   ttgatgaATGCCCAAACCAGGTTTGCTGCCAGCCCCCCCAAGGAAACCCCCTGACGCAGTGCGTAAAATCCCACATTAAACACCCATCTCGCCCCGCTTCCCGTCCCACCGCCAATAAAAACTGCTAAAAGAAGTTGTaa                                <  2:412519/139‑1 (MQ=255)                                                                                   atgatgAATGCCCAAATAAGGTATGCTGCCACCCCCCCCCACGAAATCCCCTGATGCAGTGGGTTAAATCGCATACTTAACACCCATCTCCCCACGCTCCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTaa                                <  2:204958/140‑1 (MQ=255)                                                                                    tgatgaATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGGACCACCGCCAATAAGAACTGCTAAAAGAAGTTGTAac                               >  1:311025/1‑140 (MQ=255)                                                                                    tgatgaATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGAACCACCGCCAAGAAAAACTGCTAAAAGAAGTTGTAac                               >  1:247807/1‑140 (MQ=255)                                                                                                cAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAAAAGCCATCTCGCCACGATTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATcc                   >  1:339861/1‑140 (MQ=255)                                                                                                 aaTCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCt                  >  1:411999/1‑140 (MQ=255)                                                                                                        tttGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTattt           >  2:593297/1‑140 (MQ=255)                                                                                                         ttGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCGATAAAAACGGCTAAAATAAGTTGTAGCACTTCAAAATCCTGCTatttg          >  2:195739/1‑140 (MQ=255)                                                                                                          tGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTatttga         >  1:144733/1‑140 (MQ=255)                                                                                                                 aGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTAt  >  1:629451/1‑140 (MQ=255)                                                                                                                     |                                                                                                                                        GCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTAT  >  NC_000913/657714‑657965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑