Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	669,978	G→A	P199S (CCG→TCG)	nadD ←	nicotinate‑mononucleotide adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	669,978	0	G	A	100.0%	149.4 / NA	44	P199S (CCG→TCG)	nadD	nicotinate‑mononucleotide adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/22);  total (22/22)

ACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCA  >  NC_000913/669845‑670112                                                                                                                                      |                                                                                                                                       aCCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATaa                                                                                                                                  <  2:348121/140‑1 (MQ=255)      tGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCt                                                                                                                             <  2:12360/140‑1 (MQ=255)        tAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTca                                                                                                                           <  2:81812/140‑1 (MQ=255)          aaGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTcaca                                                                                                                         <  1:556128/140‑1 (MQ=255)                aTCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTc                                                                                                                   >  2:12136/1‑140 (MQ=255)                aTCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTc                                                                                                                   >  1:455650/1‑140 (MQ=255)                  cGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCAc                                                                                                                 <  2:371031/140‑1 (MQ=255)                       ttCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTAACGATTCCTACACTATATCCTCACATGATTCACCGttt                                                                                                            >  1:214530/1‑140 (MQ=255)                               cGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGAATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACg                                                                                                    >  2:599364/1‑140 (MQ=255)                                   tCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTcg                                                                                                <  1:165466/140‑1 (MQ=255)                                          gACGAATTTACCACAGCCGCATAATGCCCTACGCAGCGAAAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGgatga                                                                                         <  2:322166/140‑1 (MQ=255)                                             gAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATgg                                                                                      <  1:442263/140‑1 (MQ=255)                                                          tCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCg                                                                                <  1:46197/133‑1 (MQ=255)                                                          tCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCg                                                                                >  2:46197/1‑133 (MQ=255)                                                                  tGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCAGATAATAGTCGCCCAGATGTTAAACCACg                                                                 >  1:204488/1‑140 (MQ=255)                                                                  tGCCCTCCGTAACGACACGTATCAGCGATACTAGCATTGCTGGTTAATTTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACg                                                                 <  2:182932/140‑1 (MQ=255)                                                                    cccTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGc                                                               >  1:58467/1‑140 (MQ=255)                                                                        ccGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGttt                                                           >  1:105883/1‑140 (MQ=255)                                                                        ccGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGttt                                                           >  1:94485/1‑140 (MQ=255)                                                                          gTAACGACAGGTATCCGCGATATAATCCTAGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCa                                                         <  1:200994/140‑1 (MQ=255)                                                                            aaCGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGc                                                       <  1:45290/140‑1 (MQ=255)                                                                             aCGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGcc                                                      <  2:598777/140‑1 (MQ=255)                                                                                   ggTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATaa                                                >  2:587491/1‑140 (MQ=255)                                                                                         aGCGATACAAGCCTTATTGGTTGATATAATTCAGTACTGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTa                                          <  1:308496/140‑1 (MQ=255)                                                                                             aTACAAGCTTTGTTGGGTAATGTAAGTCAGTACCGGTTCGGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCgg                                      <  2:328744/140‑1 (MQ=255)                                                                                             aTACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCgg                                      >  1:518895/1‑140 (MQ=255)                                                                                                   gCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCt                                <  2:619795/140‑1 (MQ=255)                                                                                                   gCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCt                                <  1:514647/140‑1 (MQ=255)                                                                                                    ccTTGTTGGTTAATGTAAGTCACTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTg                               <  1:300879/140‑1 (MQ=255)                                                                                                     cTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGa                              <  1:12136/140‑1 (MQ=255)                                                                                                        gttgGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAgg                           >  1:337934/1‑140 (MQ=255)                                                                                                          tgGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCgg                                      <  1:639771/127‑1 (MQ=255)                                                                                                          tgGTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCgg                                      >  2:639771/1‑127 (MQ=255)                                                                                                            gTTAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGaa                       <  2:5943/140‑1 (MQ=255)                                                                                                             ttAATGTAAGTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAg                      >  2:488754/1‑140 (MQ=255)                                                                                                                      gTCAGTACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCgg             >  1:649170/1‑140 (MQ=255)                                                                                                                         aGTACCGGTTCCGACAATAAATCCTCACATGTTTCGCCGGTTTGCAAAGGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGtt          <  2:204488/140‑1 (MQ=255)                                                                                                                           tACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTAt        >  2:460507/1‑140 (MQ=255)                                                                                                                           tACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTAt        >  1:466446/1‑140 (MQ=255)                                                                                                                           tACCGGTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATGTTACCGGAAGGATGAAGGTGAAGATCTTCCGGGTTAt        >  1:324355/1‑140 (MQ=255)                                                                                                                                gTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACTGGCAGGCTGAAGGTGAAGATCATCCGGGTTATGTGTc   >  1:220812/1‑140 (MQ=255)                                                                                                                                gTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTc   >  2:452983/1‑140 (MQ=255)                                                                                                                                gTTCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTc   >  1:448886/1‑140 (MQ=255)                                                                                                                                 ttCCGACAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTTAAGGTGCAGATCTTCCGGGTTATGTGTCa  >  1:269779/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       ACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCA  >  NC_000913/669845‑670112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑