Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 679,673 A→G T56A (ACT→GCT)  ybeU → DUF1266 domain‑containing protein YbeU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913679,6730AG97.7% 150.4 / ‑4.5 44T56A (ACT→GCT) ybeUDUF1266 domain‑containing protein YbeU
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (20/23);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CGCTTTCTGCGCCCATGGAAATACTTAATCAGGGTTGCAAACCCGCGCATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGACTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCC  >  NC_000913/679539‑679805
                                                                                                                                      |                                                                                                                                    
cgcTTTCTGCGCCCATGGAAATACTTAATCAGGGTTGCAAACCCGCGCATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTAtt                                                                                                                                 <  1:93632/140‑1 (MQ=255)
   tttCTGCGCCCATGGCAATACTTAAGCAGGGTTGCAATCCCGCGCATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTAt                                                                                                                              <  1:277096/140‑1 (MQ=255)
   tttCTGCGCCCATGGAAATACTTAATCAGGGTTGCAAACCCGCGCATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTAt                                                                                                                              <  2:544183/140‑1 (MQ=255)
    ttCTGCGCCCATGGAAATACTTAATCAGGGTTGCAAACCCGCGCATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGCGAACAAATAATCGTGATGATTCAATTCAGGCTATTTATc                                                                                                                             >  2:277096/1‑140 (MQ=255)
     tCTGCGCCCATGGAAATACTTAATCAGGGTTGCAAACCCGCGCATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCa                                                                                                                            <  1:625414/140‑1 (MQ=255)
                ggAAATACTTAATCAGGGTTGCAAACCCGCGCATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACtgatg                                                                                                                 <  2:347003/140‑1 (MQ=255)
                     tACTTAATCAGGGTTTCAAACCCGCCCATCACTCGCCTAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGt                                                                                                            <  1:197300/140‑1 (MQ=255)
                        ttAATCAGGGTTGCAAACCCGCGCATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCAt                                                                                                         <  2:36577/140‑1 (MQ=255)
                          aaTCAGGGTTGCAAACCCGCGCATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGc                                                                                                       >  2:81341/1‑140 (MQ=255)
                           aTCAGGGTTGCAAACCCGCGCATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGCGATGATTTCATTAAGGGTATTTATCCGATGACTGATGATGGTCATGcc                                                                                                      >  2:249244/1‑140 (MQ=255)
                                 gTTGCAAACCCGCGCATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGt                                                                                                            >  2:360522/1‑128 (MQ=255)
                                 gTTGCAAACCCGCGCATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGt                                                                                                            <  1:360522/128‑1 (MQ=255)
                                 gTTGCAAACCCGCGCATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGAt                                                                                                >  1:543991/1‑140 (MQ=255)
                                 gTTGCAAACCCGCGCATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGATATTTATCAGAGGAATGATGATGGTCATGCCAATGAt                                                                                                >  1:219713/1‑140 (MQ=255)
                                     cAAACCCGCGCATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAg                                                                                            <  1:121422/140‑1 (MQ=255)
                                      aaaCCCGCTCATGACTTGCCAAAAATGTATCCTGGCATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGc                                                                                           <  2:314825/140‑1 (MQ=255)
                                          ccGCGCATGACTCGCTAAAAATGTAAACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGc                                                                                       <  1:327428/140‑1 (MQ=255)
                                          ccGCGCATGACTCGCCTAAAATTTATACTGGGATATAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGc                                                                                       <  2:298807/140‑1 (MQ=255)
                                            gcgcATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGAGGATGGTCATGCCAATGATTTAGCTGGCtt                                                                                     >  1:298807/1‑140 (MQ=255)
                                             cgcATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATTGTCATGCCAATGATTTAGCTGGCTta                                                                                    >  1:584015/1‑140 (MQ=255)
                                             cgcATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTta                                                                                    >  2:468491/1‑140 (MQ=255)
                                             cgcATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCACATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTta                                                                                    >  2:419691/1‑140 (MQ=255)
                                                 tGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATc                                                                                <  1:565434/140‑1 (MQ=255)
                                                  gACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCt                                                                               >  2:579147/1‑140 (MQ=255)
                                                                 tataCTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGctc                                                                <  2:543991/140‑1 (MQ=255)
                                                                 tataCTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGctc                                                                <  1:343560/140‑1 (MQ=255)
                                                                 tataCTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGctc                                                                <  2:509140/140‑1 (MQ=255)
                                                                        ggATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCt                                                         >  1:33919/1‑140 (MQ=255)
                                                                         gATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTg                                                        >  2:150240/1‑140 (MQ=255)
                                                                                 aaTTCGAGCTCTCATCATCCTGGGGAATAAAGAATTGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTgg                                                <  2:273014/140‑1 (MQ=255)
                                                                                   ttCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGaa                                              >  1:650512/1‑140 (MQ=255)
                                                                                                      ggggAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGaaa                                             <  1:149553/122‑1 (MQ=255)
                                                                                                      ggggAATAAATAATCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGaaa                                             >  2:149553/1‑122 (MQ=255)
                                                                                                       gggAATAAATAATCGTGATGATCTAATTCAGGCTATTGATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCt                          <  1:249244/140‑1 (MQ=255)
                                                                                                            taaataaTCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGgag                     >  1:650363/1‑140 (MQ=255)
                                                                                                            taaataaTCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGga                      <  2:384988/139‑1 (MQ=255)
                                                                                                            taaataaTCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGga                      >  1:384988/1‑139 (MQ=255)
                                                                                                             aaataaTCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATTGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGgaga                    <  2:584015/140‑1 (MQ=255)
                                                                                                             aaataaTCGTGATGATTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGgaga                    <  2:123440/140‑1 (MQ=255)
                                                                                                             aaataaTCGTGATGATTTAATTCAGACTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGgaga                    >  1:159869/1‑140 (MQ=255)
                                                                                                                       gatgatTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGAt          <  1:468491/140‑1 (MQ=255)
                                                                                                                        atgatTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGAtt         >  2:441564/1‑140 (MQ=255)
                                                                                                                        atgatTTAATTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGAtt         >  1:18252/1‑140 (MQ=255)
                                                                                                                               aaTTCAGGCTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATAccc  >  2:487831/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                    
CGCTTTCTGCGCCCATGGAAATACTTAATCAGGGTTGCAAACCCGCGCATGACTCGCCAAAAATGTATACTGGGATAAAAGAATTCGAGCTCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGACTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCC  >  NC_000913/679539‑679805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑