Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	710,861	A→G	intergenic (‑136/+74)	uof ← / ← fldA	RyhB‑regulated fur leader peptide/flavodoxin 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	710,861	0	A	G	100.0%	214.3 / NA	59	intergenic (‑136/+74)	uof/fldA	RyhB‑regulated fur leader peptide/flavodoxin 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/33);  total (26/33)

TTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTT  >  NC_000913/710733‑710993                                                                                                                                 |                                                                                                                                     ttGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAg                                                                                                                           >  1:554777/1‑140 (MQ=255) ttGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAg                                                                                                                           >  2:46465/1‑140 (MQ=255) ttGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAg                                                                                                                           >  1:419611/1‑140 (MQ=255) ttGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAg                                                                                                                           >  2:558582/1‑140 (MQ=255) ttGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATAGTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAg                                                                                                                           >  2:421127/1‑140 (MQ=255)   gCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCa                                                                                                                         >  2:48953/1‑140 (MQ=255)   gCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCa                                                                                                                         >  2:624791/1‑140 (MQ=255)    ccACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCaa                                                                                                                        >  2:598288/1‑140 (MQ=255)    ccACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCaa                                                                                                                        <  2:427587/140‑1 (MQ=255)        gCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAattat                                                                                                                    <  1:613790/140‑1 (MQ=255)         ccTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAattatt                                                                                                                   >  2:459103/1‑140 (MQ=255)         ccTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTCGTAGCAattatt                                                                                                                   >  1:16848/1‑140 (MQ=255)             aaTGAAAACCACAAGTCGCTGGAAAATATTGTTAAGAAAGACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGa                                                                                                               <  2:228226/140‑1 (MQ=255)             aaTGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGa                                                                                                               <  1:48953/140‑1 (MQ=255)             aaTGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGa                                                                                                               >  2:73875/1‑140 (MQ=255)             aaTGAAAACCACAAGGCCCTGGCAATTATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGa                                                                                                               <  1:322505/140‑1 (MQ=255)              aTGAAAACTACAAGTCCCTGGCTAATATTGTTATTCAAACCGTGGCACCGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGAt                                                                                                              <  2:302806/140‑1 (MQ=255)              aTGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGAt                                                                                                              <  1:7197/140‑1 (MQ=255)                  aaaCCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCCGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGc                                                                                                          >  1:537126/1‑140 (MQ=255)                   aaCCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCa                                                                                                         <  1:22523/140‑1 (MQ=255)                   aaCCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCa                                                                                                         <  2:419611/140‑1 (MQ=255)                   aaCCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCa                                                                                                         <  1:541741/140‑1 (MQ=255)                     ccACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCAtt                                                                                                       <  1:4452/140‑1 (MQ=255)                     ccACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCAtt                                                                                                       <  1:345869/140‑1 (MQ=255)                      cacaAGTCCCTGGCAAATATTGTTAATAATAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTa                                                                                                      <  1:265335/140‑1 (MQ=255)                      cacaAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTa                                                                                                      <  1:564072/140‑1 (MQ=255)                      cacaAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTa                                                                                                      <  2:590632/140‑1 (MQ=255)                        caAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATc                                                                                                    <  1:459103/140‑1 (MQ=255)                               cTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAAt                                                                                             <  1:624791/140‑1 (MQ=255)                                tGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAAt                                                                                             >  2:465969/1‑139 (MQ=255)                                tGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAAt                                                                                             <  1:465969/139‑1 (MQ=255)                                tGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATa                                                                                            <  2:448690/140‑1 (MQ=255)                                tGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATa                                                                                            >  1:448690/1‑140 (MQ=255)                                tGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATa                                                                                            <  2:286209/140‑1 (MQ=255)                                tGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATa                                                                                            <  2:400225/140‑1 (MQ=255)                                tGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTCGTAGCAATTATTTTGATTGGCATTATCTATTAATa                                                                                            <  2:16848/140‑1 (MQ=255)                                    aaaTATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGc                                                                                        <  2:602948/140‑1 (MQ=255)                                      atatTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGt                                                                                      >  1:452099/1‑140 (MQ=255)                                       tatTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCCGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTa                                                                                     <  2:537126/140‑1 (MQ=255)                                            ttAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACAt                                                                                >  1:35123/1‑140 (MQ=255)                                            ttAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACAt                                                                                <  1:73875/140‑1 (MQ=255)                                                         ggCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATCTTGATTGGCATTATCTATTAATACGTCGTAGACATGAGTCTAcgccgt                                                                   >  2:280070/1‑139 (MQ=255)                                                          gCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGca                                                                  <  1:598288/140‑1 (MQ=255)                                                                cTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAGTTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGcatcacat                                                            <  1:421127/140‑1 (MQ=255)                                                                     aTTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGc                                                       <  1:555782/140‑1 (MQ=255)                                                                         aCAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGca                                                                  >  2:622325/1‑125 (MQ=255)                                                                         aCAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGca                                                                  <  1:622325/125‑1 (MQ=255)                                                                          cAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTga                                                  >  2:432644/1‑140 (MQ=255)                                                                                     ccACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTtcgtcg                                       <  2:452099/140‑1 (MQ=255)                                                                                     ccACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTtcgtcg                                       <  2:74677/140‑1 (MQ=255)                                                                                        cTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCgaga                                    >  2:417681/1‑140 (MQ=255)                                                                                             acacCGGGACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCaa                               <  2:554777/140‑1 (MQ=255)                                                                                                   ggACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTc                         >  1:339584/1‑140 (MQ=255)                                                                                                   ggACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTc                         >  1:344665/1‑140 (MQ=255)                                                                                                   ggACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTc                         >  2:82149/1‑140 (MQ=255)                                                                                                    gACATTGTACAGGTACAACAGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCa                        <  2:35123/140‑1 (MQ=255)                                                                                                                   caacaGCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAAcc         >  1:654353/1‑140 (MQ=255)                                                                                                                        gCAAAAGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCAttt    >  2:14308/1‑140 (MQ=255)                                                                                                                          aaaaGTTGCAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCAttttt  >  2:607627/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                     TTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATACGGCGTAGACATGAGTCTACGCCGCATCACATCAGGCATTGAGAATTTCGTCGAGATGCAACTCTTCAGAAATCTGTTTAACCCATTTTT  >  NC_000913/710733‑710993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑