Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	721,836	C→T	G860E (GGG→GAG)	kdpD ←	sensory histidine kinase KdpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	721,836	0	C	T	100.0%	75.4 / NA	24	G860E (GGG→GAG)	kdpD	sensory histidine kinase KdpD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/11);  total (13/11)

TGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCCCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTACCCCCGGTACTGCCGACTCTTTATTCCCGCGAGCAAACTTATCAAATATCGTCTGCTCCTGGCCTGGCGGAAGACCGG  >  NC_000913/721702‑721959                                                                                                                                       |                                                                                                                            tGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCgtgt                                                                                                                        <  2:81060/140‑1 (MQ=255) tGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCgtgt                                                                                                                        <  2:496538/140‑1 (MQ=255)    tCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACa                                                                                                                     >  1:472196/1‑140 (MQ=255)      aTCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATc                                                                                                                   <  2:561799/140‑1 (MQ=255)        cTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCa                                                                                                                 >  2:650436/1‑140 (MQ=255)                 tCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGccc                                                                                                        <  2:158443/140‑1 (MQ=255)                               acaTATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGt                                                                                          >  2:334128/1‑140 (MQ=255)                                   atCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCaa                                                                                      >  2:409167/1‑140 (MQ=255)                                    tCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAg                                                                                     >  2:400782/1‑140 (MQ=255)                                      cTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGcc                                                                                   <  1:440644/140‑1 (MQ=255)                                       tCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGccc                                                                                  >  2:147167/1‑140 (MQ=255)                                        cATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCt                                                                                 >  1:453267/1‑140 (MQ=255)                                        cATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCt                                                                                 <  2:6027/140‑1 (MQ=255)                                           gAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTAcc                                                                              >  1:625823/1‑140 (MQ=255)                                               ttcttcAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTACCCCCg                                                                          >  1:410362/1‑140 (MQ=255)                                                       gTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCAACAAATTGCCAGTCTAAGCCCGACCCCCGGTACTGcc                                                                  >  1:308483/1‑140 (MQ=255)                                                                        tGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCAAAGGCCTAGCCCCGGTACTGCCGACTCTTTATTCCcgcg                                                 >  2:317252/1‑140 (MQ=255)                                                                             aaGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTACCCCCGGTACTGCCGACTCTTTATTCCCGCGAGCaa                                            <  2:27877/140‑1 (MQ=255)                                                                                        gAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTACCCCCGGTACTGCCGACTCTTTATTCCCGCGAGCAAACTTATCAAat                                 <  2:410362/140‑1 (MQ=255)                                                                                         aaaaCAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTACCCCCGGTACTGCCGACTCTTTATTCCCGCGAGCAAACTTATCAAata                                >  1:661259/1‑140 (MQ=255)                                                                                              aGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTACCCCCGGTACTGCCGACTCTTTATTCCCGCGAGCAAACTTATCAAATATCGTc                           <  2:453267/140‑1 (MQ=255)                                                                                                                 gtTGAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTACCCCCGGTACTGCCGACTCTTTAATCCCGCGAGCAAGCTTTTCAAATATGGTCTGCTCCTGGCCTGGCGGaa        >  2:175768/1‑140 (MQ=255)                                                                                                                    gAACGCGGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTACCCCCGGTACTGCCGACTCTTTATTCCCGCGAGCAAACTTATCAAATATCGTCTGCTCCTGGCCTGGCGGAAGAc     <  2:472196/140‑1 (MQ=255)                                                                                                                       cgcgGTAATAGTGCCCTCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTACCCCCGGTACTGCCGACTCTTTATTCCCGCGAGCAAACTTATCAAATATCGTCTGCTCCTGGCCTGGCGGAAGACCgg  <  1:650436/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                            TGTTCATCTTCAACAATCAGAACGTTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCCCGTGTACATCCACTATCGCCCGACAAATTGCCAGTCCAAGCCCTACCCCCGGTACTGCCGACTCTTTATTCCCGCGAGCAAACTTATCAAATATCGTCTGCTCCTGGCCTGGCGGAAGACCGG  >  NC_000913/721702‑721959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑