Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	757,649	C→T	F581F (TTC→TTT)	sdhA →	succinate:quinone oxidoreductase, FAD binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	757,649	0	C	T	100.0%	109.1 / NA	35	F581F (TTC→TTT)	sdhA	succinate:quinone oxidoreductase, FAD binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/16);  total (19/16)

GTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTTGATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGA  >  NC_000913/757516‑757783                                                                                                                                      |                                                                                                                                       gTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACCCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTccgccg                                                                                                                                  <  1:499867/140‑1 (MQ=255)  tGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGa                                                                                                                                 >  1:337975/1‑140 (MQ=255)     cgcgcATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGa                                                                                                                              >  2:433229/1‑140 (MQ=255)      gcgcATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGAt                                                                                                                             <  2:48828/140‑1 (MQ=255)          aTAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGt                                                                                                                         <  2:492317/140‑1 (MQ=255)              ccGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCTGCGTTTCCGGCGAAGATTCGtactt                                                                                                                     >  1:185471/1‑140 (MQ=255)                  ttCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGAt                                                                                                                             >  2:619627/1‑128 (MQ=255)                  ttCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGAt                                                                                                                             <  1:619627/128‑1 (MQ=255)                  ttCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTa                                                                                                                 >  2:367656/1‑140 (MQ=255)                           ccGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGgaga                                                                                                        <  1:41916/140‑1 (MQ=255)                                    gatgatgaAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAAt                                                                                               >  1:94334/1‑140 (MQ=255)                                         tgaAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGAc                                                                                          >  1:346629/1‑140 (MQ=255)                                         tgaAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCACTTCCGCCGAAGACTCGACCTTACTAATGCGGAGACAGGAAAATGAGAc                                                                                          >  2:307976/1‑140 (MQ=255)                                          gaAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACt                                                                                         <  2:415515/140‑1 (MQ=255)                                                  gCTGTGCCACTCCCTGTCTCTGTCAGAGTCGAAATTCATCACGCGCCGCAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGtttt                                                                                 <  2:185471/140‑1 (MQ=255)                                                  gCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTGCTATTGCGGAGAGAGGAGAATGAGACTCGAGtttt                                                                                 >  2:214084/1‑140 (MQ=255)                                                     gtgCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCaa                                                                              >  1:134151/1‑140 (MQ=255)                                                         cACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTa                                                                          <  1:367656/140‑1 (MQ=255)                                                             cccTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGc                                                                      <  2:346629/140‑1 (MQ=255)                                                              ccTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCt                                                                     >  1:282130/1‑140 (MQ=255)                                                                     cTGCCAGAGTCGGAAGCCATGATGCGCCGAAGCGTCATCATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAAccc                                                              <  1:224687/140‑1 (MQ=255)                                                                      tGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCg                                                             >  1:434563/1‑140 (MQ=255)                                                                       gCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCga                                                            >  1:29895/1‑139 (MQ=255)                                                                        ccAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGa                                                           >  2:529404/1‑140 (MQ=255)                                                                            agTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGtt                                                       >  1:610368/1‑140 (MQ=255)                                                                              tCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTtga                                                     >  1:10069/1‑140 (MQ=255)                                                                              tCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTtga                                                     >  1:519671/1‑140 (MQ=255)                                                                                              gcCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTTGATGATGCTCCGCGTATg                                     <  2:134151/140‑1 (MQ=255)                                                                                              gcCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGACTTTTCAATTTATAGCTATAACCCGGCTGTTGATGATGCTCCGCGTATg                                     >  2:259388/1‑140 (MQ=255)                                                                                               ccGAAGCGTCAACATGGAACCGTAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTTGATGATGCTCCGCGTATGc                                    <  2:282130/140‑1 (MQ=255)                                                                                                      gTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTTGATGATGCTCCGCGTATGCAGGATTa                             <  2:337975/140‑1 (MQ=255)                                                                                                      gTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTTGATGATGCTCCGCGTATGCAGGATTa                             <  2:546705/140‑1 (MQ=255)                                                                                                      gTCAACATGGAACCGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTTGATGATGCTCCGCGTATGCAGGATTa                             <  1:619246/140‑1 (MQ=255)                                                                                                               gAACCGAAACTGCGCTCGGCATATCTGCCAAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTTGATGATGCTCCGCGTATGCAGGATTACACCCTGGa                    <  1:307976/140‑1 (MQ=255)                                                                                                                   cGAAACTGCGCCCGGCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTTGATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCg                >  2:166052/1‑140 (MQ=255)                                                                                                                                 gCATTTCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTTGATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGa  <  2:354856/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       GTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTTGATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGA  >  NC_000913/757516‑757783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑