Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	771,282	A→G	intergenic (+671/‑176)	mngB → / → cydA	alpha‑mannosidase/cytochrome bd‑I ubiquinol oxidase subunit I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	771,282	0	A	G	100.0%	129.0 / NA	36	intergenic (+671/‑176)	mngB/cydA	alpha‑mannosidase/cytochrome bd‑I ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/22);  total (14/22)

CATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTA  >  NC_000913/771145‑771411                                                                                                                                          |                                                                                                                                  cATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCg                                                                                                                                 <  2:78367/140‑1 (MQ=255)     aaaaaGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTggg                                                                                                                             <  2:151639/140‑1 (MQ=255)      aaaaGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGa                                                                                                                            <  1:602056/140‑1 (MQ=255)            gaTAAATTGTTCTCGCTCAAATAGGATGAGAGGCGGTCATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAAtt                                                                                                                      <  1:312199/140‑1 (MQ=255)                 aTTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGt                                                                                                                 <  1:512038/140‑1 (MQ=255)                   tGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGttt                                                                                                               <  1:9979/140‑1 (MQ=255)                     ttCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGttttt                                                                                                             >  2:505355/1‑140 (MQ=255)                       ctcGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTttat                                                                                                           >  1:574059/1‑140 (MQ=255)                        tcGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTttatt                                                                                                          <  1:366870/140‑1 (MQ=255)                            tCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTTATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACa                                                                                                      <  1:283263/140‑1 (MQ=255)                               aaTTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGt                                                                                                   >  1:546781/1‑140 (MQ=255)                                aTTGGCTGAAAGGCGATAATTTCGCGATACATTGATCCCCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGtt                                                                                                  <  1:184505/140‑1 (MQ=255)                                  tGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTg                                                                                                <  1:546447/140‑1 (MQ=255)                                      tGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAAc                                                                                            >  1:150918/1‑140 (MQ=255)                                       gAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAAcc                                                                                           <  1:19827/140‑1 (MQ=255)                                                 aaTTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACg                                                                                 <  1:152432/140‑1 (MQ=255)                                                      aGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGtt                                                                            >  2:358752/1‑140 (MQ=255)                                                            aaaTTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTtgtg                                                                      <  1:376290/140‑1 (MQ=255)                                                              aTTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAACACCTGTTTTTTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTtgtgtg                                                                    <  2:236139/140‑1 (MQ=255)                                                                tGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCa                                                                  <  1:358752/140‑1 (MQ=255)                                                                     aCCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTcac                                                             >  2:407208/1‑140 (MQ=255)                                                                      ccGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTcaca                                                            <  2:546781/140‑1 (MQ=255)                                                                       cGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACAt                                                           >  2:74231/1‑140 (MQ=255)                                                                       cGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACAt                                                           >  1:454442/1‑140 (MQ=255)                                                                       cGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTAGTAACATGTTTGCGACCTTTCTTTACGCCGTGTTTGTGTGCATTCACAt                                                           >  2:187082/1‑140 (MQ=255)                                                                         tCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATgg                                                         <  1:342871/140‑1 (MQ=255)                                                                                  gCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGaa                                                <  1:76958/140‑1 (MQ=255)                                                                                                 caaAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAAt                                 <  2:58503/140‑1 (MQ=255)                                                                                                     aaCCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTcaaa                                      >  2:72300/1‑131 (MQ=255)                                                                                                     aaCCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTcaaa                                      <  1:72300/131‑1 (MQ=255)                                                                                                     aaCCTGTTTATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCt                             >  2:432479/1‑140 (MQ=255)                                                                                                           tttATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTggg                       >  1:518709/1‑140 (MQ=255)                                                                                                             tATTGTAAGGATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGc                     <  2:520850/140‑1 (MQ=255)                                                                                                                     ggATTTTGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCgtgt             >  2:137831/1‑140 (MQ=255)                                                                                                                        ttttGCGGCGTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGAc          <  2:150918/140‑1 (MQ=255)                                                                                                                                cgTAATATATGCGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTa  >  2:538920/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                  CATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATTGTAAGGATTTTGCGGCGTAATATATACGTGGGATCAATTTGAGTTTTTATTAACATGTTTGCAACCTTTCTTTACGCCGTTTTTGTGTGCATTCACATGGTATGATGAAAGTGTTCAAACAAATTTCTATTGGGGCATGCGTGTGACCCTTTCTA  >  NC_000913/771145‑771411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑