| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 825,051 | G→A | A353V (GCC→GTC) | ybhR ← | putative ABC exporter membrane subunit YbhR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 825,051 | 0 | G | A | 97.1% | 113.3 / ‑6.7 | 34 | A353V (GCC→GTC) | ybhR | putative ABC exporter membrane subunit YbhR |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (10/23); minor base T (1/0); total (11/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGG > NC_000913/824915‑825184 | cTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCCACACATCGCGTAGGCCGTTGACCCTGCCGTGACCg > 2:325862/1‑140 (MQ=255) tGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGt > 2:331599/1‑140 (MQ=255) ggAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGtt < 2:68239/140‑1 (MQ=255) cTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCTGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGcc < 1:484803/140‑1 (MQ=255) cTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGcc < 1:531223/140‑1 (MQ=255) tGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCca < 1:533362/140‑1 (MQ=255) gCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCcaca < 1:522949/140‑1 (MQ=255) aaTTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGCAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACaaa < 1:332940/140‑1 (MQ=255) cAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTAtt < 1:331599/140‑1 (MQ=255) gggCTGGCAATCCTTGCTGGCCCGGCGGTAGGGTCTTTGCTGGCGAAACATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTcc < 2:201705/140‑1 (MQ=255) gggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTcc < 1:558825/140‑1 (MQ=255) gggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTcc < 1:631253/140‑1 (MQ=255) gggCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTcc < 2:93503/140‑1 (MQ=255) tGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCcacac < 2:373784/140‑1 (MQ=255) ggCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCcacaca < 1:612301/140‑1 (MQ=255) gCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGTCCGTTATTACCAGTAGAGGCCTCAAACTATTCCACACaa > 2:289334/1‑140 (MQ=255) gCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACaa > 1:577041/1‑140 (MQ=255) cccggcGGTAGTGTCTTTGCTGGCAAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCa > 2:86350/1‑140 (MQ=255) gcggTAGTGTCTTTGCTTGCGACAGATAAGATGTTTCATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCt < 2:259803/140‑1 (MQ=255) gcggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCt < 1:106039/140‑1 (MQ=255) gCTGGCGAAAGATAAGAAGTTACATCACCTTGCGTTTAATCATCGCGTCCGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGc < 2:259778/140‑1 (MQ=255) cGAAAGATAAGAAGTCACATCAGCTGACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGt < 1:283189/140‑1 (MQ=255) gAAAGATAAGAAGTTACATCACTTTCCGTCTAAACATTGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTa < 1:325862/140‑1 (MQ=255) aaaGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTaa < 2:508676/140‑1 (MQ=255) aCATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCGTCCCTCAAATAAATCTACTTGGTAATGTCCGTAAAGTgg > 2:270058/1‑140 (MQ=255) cACCTTACGTCTAAAGATCGCGGACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa < 1:214298/140‑1 (MQ=255) cACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa < 1:31744/140‑1 (MQ=255) aCCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt < 2:88081/140‑1 (MQ=255) cGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGtt > 2:503306/1‑140 (MQ=255) tAAACATCGCGTACGGCGCTGACCCTGTCTTGACCGTAATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATc < 1:270058/140‑1 (MQ=255) aaaCATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATcc > 2:492094/1‑140 (MQ=255) aaCATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCa > 2:247218/1‑140 (MQ=255) aaCATCGCGTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCa > 2:426071/1‑140 (MQ=255) gTACGCCGCTGACCCTGTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGt > 2:145795/1‑140 (MQ=255) gTCGTGACCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATACTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCgg > 1:531118/1‑140 (MQ=255) | CTGGAAAACAACTTTTTGCTGGCAATTGCAGGGCTGGCAATCCTTGCCGGCCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGG > NC_000913/824915‑825184 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |